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   在分化 的翻译结果: 查询用时:0.505秒
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在分化
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  in differentiation
     in differentiation better group was 56.3% and 68.8%,in differentiation worse group was 37.7%( χ 2=1.40,P<0.05) and 17.2%(χ 2=11.94,P<0.01) .
     在分化好组VEGF、ER表达分别为 5 6.3 %和 68.8% ,而分化差组分别为 3 7.9% (χ2 =1.40 ,P <0 .0 5 )和 17.2 % (χ2 =11.94,P <0 .0 1)。
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     To investigate the possible involvement of Ras signaling in the hematopoietic differentiation of embryonic stem cells (ES cells), ES cells were transfected with RasN17, the dominant-negative mutant of Ras. Western blot was used to test the effect of RasN17 expression on Erk1/2 and Akt phosphorylation, semi-quantitative RT-PCR was used to detect expression of gene related to hemalopoiesis in differentiation of ES cells.
     为了探讨Ras信号通路对小鼠胚胎干细胞(Embryonic Stem cells,ES cells)造血分化的影响,将突变型基因RasN17转染小鼠ES细胞,免疫印迹检测RasN17的表达对Erk1/2及Akt磷酸化的作用,应用半定量RT-PCR检测ES细胞在分化过程中与造血相关的基因的表达。
短句来源
     About 25.0% to 95.0% of green sprout formed in all of differentiated calli, the regenerating plantlets were only obtained from calli induced via hypocotyl in vitro cultured, and the differentiation rate was up to 20.0% which in differentiation medium containing MS +100 mg/L casein hydrolysate+0.1 mg/L α-NAA+3.0 mg/L 6-BA.
     在所有愈伤分化试验中均形成了绿色芽点 ,其频率为 2 5 .0 %~ 95 .0 % ,仅从下胚轴诱导的愈伤分化获得了再生植株 ,且在分化培养基 (MS +1 0 0mg/L水解酪蛋白 +0 .1mg/Lα -NAA +3.0mg/L 6 -BA)上的最高愈伤分化频率为 2 0 .0 %。
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     3. The transgenic system of Populus simoniiX P. nigra plantlets obtained from pollen was set up:Pre-culture: the leaves cultured in differentiation medium (MH+6-BA 0.5mg L-1+NAA 0.05 mgL-1+sucrose 25 g L-1+agar 6 g L-1) for 2days ;
     (3) 建立了以小黑杨花粉植株叶片为外植体的转化体系: 预培养:在分化培养基(MH+6-BA0.5mg·L~(-1)+NAA0.05mg·L~(-1)+蔗糖25g·L~(-1)+琼脂6g·L~(-1))
短句来源
     Northern blot analysis indicated that MLP gene was transcribed from the third day when C2C12 cells were cultured in differentiation medium.
     以此cDNA为探针进行的Northern印迹表明 ,MLP于C2C12细胞在分化的第 3d至第 5d表达 .
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  “在分化”译为未确定词的双语例句
     Callus was differentiated easily in media consisted of MS+1.0 mg·L~(-1) 6-BA+0.1 mg·L~(-1) NAA, MS+1.0 mg·L~(-1) 6-BA+0.5 mg·L~(-1) NAA(The differentiation rates were 86% and 52% respectively).
     在分化培养基MS+1.0mg·L-16 BA+0.1mg·L-1NAA及MS+1.0mg·L-16 BA+0.5mg·L-1NAA上,愈伤组织容易分化(分化效率分别为86%和52%)。
短句来源
     But CD99 expression was 95.0%(20/21) in EWS/PNET,71.4%(5/7) in poorly differentiated synovial sarcomas,66.7%(8/9) in rhabdomyosarcomas,75.0%(3/4) in esthesioneuroblastomas and 60%(3/5) in mesenchymal chondrosarcomas,respectively.
     CD99在EW S/PNET中阳性表达率为95.2%(20/21),在分化差的滑膜肉瘤、横纹肌肉瘤、嗅神经母细胞瘤和间叶软骨肉瘤分别为71.4%(5/7)、66.7%(6/9)、75.0%(3/4)和60%(3/5)。
短句来源
     Expression of adiponutrin mRNA in mature 3T3-L1 adipocytes
     Adiponutrin mRNA在分化成熟的3T3-L1脂肪细胞中的表达
短句来源
     E2 strongly stimulated the expression of PPAR-γ2 mRNA in bone marrow stromal cell from ( 1.80± 0.3)% to (9.5±2.1)% (P<0.01) and (19.3±3.0)% (P<0.01).
     E2能明显促进骨髓基质细胞在分化介质中PPAR-γ2mRNA的表达,在上述E2浓度时,PPAR-γ2mRNA的表达从(1.80±0.3)%增至(9.5±2.1)%(P<0.01)和(19.2±3.0)%(P<0.01);
短句来源
     E2 strongly stimulated the expression of PPAR-γ2 mRNA in bone marrow stromal cell from(1.75±0.5)% to(9.5±2.1)%(P<0.01)and(19.3±3.0)%(P<0.01).
     E2能明显促进骨髓基质细胞在分化介质中PPAR-γ2 mRNA的表达,在上述E2浓度时,PPAR-γ2 mRNA的表达从(1.75±0.5)%增至(9.5±2.1)%(P<0.01)和(19.3±3.0)%(P<0.01);
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  相似匹配句对
     Differentiation- of Thymocytes
     胸腺细胞的分化
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     biauraria branched off, D.
     biauraria的分化 ,D .
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  in differentiation
The Role of Stem and Pluripotent Cells in Differentiation and the Neoplastic Transformation of Pancreatic Cells
      
Redusomes are hypothetical perichromosomal particles arising in differentiation events during morphogenesis of an organism.
      
Transcription factor Oct-1 is ubiquitous, participating in expression of the cell housekeeping genes as well as in differentiation of lymphocytes and activation of transcription of immunoglobulin genes in B cells.
      
A review of the mechanisms underlying cytogenesis of the nervous tissues, the role of the microenvironment, cell interactions in differentiation, and plastic rearrangements in the mammalian brain after transplantation of the embryonic nervous tissue.
      
It has been proposed that the cell with microvilli represent a transitional stage in differentiation of the ciliary cells.
      
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Isoenzyme patterns of peroxidase,catalase,glucose-6-phosephate,glutamate and isocitrate dehydrogenases,esterase,amylase and IAA oxidase in the embryos,endos- perms,roots and shoots of wheat seedlings(Triticum aestivum L.var.Nung-da 139) were determined by horizontal starch gel electrophoresis and polyacrylamide gel disc electrophoresis respectively.The number of isoenzymes of peroxidase and amylase was increased with the concomitant increase of days during germination.The isoen- zyme bands of esterase,glutamate,glucose-6-phosphate...

Isoenzyme patterns of peroxidase,catalase,glucose-6-phosephate,glutamate and isocitrate dehydrogenases,esterase,amylase and IAA oxidase in the embryos,endos- perms,roots and shoots of wheat seedlings(Triticum aestivum L.var.Nung-da 139) were determined by horizontal starch gel electrophoresis and polyacrylamide gel disc electrophoresis respectively.The number of isoenzymes of peroxidase and amylase was increased with the concomitant increase of days during germination.The isoen- zyme bands of esterase,glutamate,glucose-6-phosphate and isocitrate dehydrogenases in the embryos were more in the begining of germination.The activities of pero- xidase,IAA oxidase and glutamate dehydrogenase in roots were higher than those in shoots.On the contrary,the activities of catalase and glucose-6-phosphate dehydrogenase in shoots were higher than those in roots.However the activity of esterase was slight higher in shoots.There was no difference in the activity of isocitrate dehydrogenase between roots and shoots.The morphological difference of shoot and root is evidently related to isoenzyme patterns.This investigation indi- cates that different metabolic characters are existed in shoot and root during dif- ferentiation.

利用水平板淀粉凝胶电泳法和盘状聚丙烯酰胺凝胶电泳法,结合酶的染色法研究了小麦种子萌发后的胚、幼根和幼苗中过氧化物酶等6种同工酶及胚乳中淀粉酶同工酶的变化;测定了幼根和幼苗中过氧化物酶和吲乙酸氧化酶的活性。过氧化物酶和淀粉酶的同工酶数目随萌发大量增加。6-磷酸葡萄糖脱氢酶、谷氨酸脱氢酶、异柠檬酸脱氢酶和酯酶在萌发初期的胚中就有不少同工酶带和较强的活性。过氧化物酶、吲乙酸氧化酶和谷氨酸脱氢酶在根中比在苗中活性大得多,相反,过氧化氢酶和6-磷酸葡萄糖脱氢酶在苗中比在根中活性大得多,酯酶在苗中比在根中活性稍大,只有异柠檬酸脱氢酶在根和苗中无甚差异。同工酶带的数目及其相对强度的不同可能与器官差异有关。此研究有助于进一步探讨不同组织和器官在分化时具有不同的代谢特点。

The treated leaf explants of Nicotiana tabacum were used as the experimental material. Chosiug leaves of the mutagenic plants (79-1, 79-2, 79-5,), its flower coloure changes, and of check plants (the condition of differentiation, transplanting, bloom and the age of leaf were similar to the mutagenic plant) were used for the determination of the peroxidase isozyme pattern by polyacrylamide gel electrophoresis. The results were illustrated in Fig. 1,2. In the older leaves of the control plant there were two extra...

The treated leaf explants of Nicotiana tabacum were used as the experimental material. Chosiug leaves of the mutagenic plants (79-1, 79-2, 79-5,), its flower coloure changes, and of check plants (the condition of differentiation, transplanting, bloom and the age of leaf were similar to the mutagenic plant) were used for the determination of the peroxidase isozyme pattern by polyacrylamide gel electrophoresis. The results were illustrated in Fig. 1,2. In the older leaves of the control plant there were two extra peroxidase isozyme bands than that of the mutagenic plant at the region of slowly migrating rate. However, there was no obvious difference between the control and treated plant in the younger leaves. When the differentiation of the explants irradiated by ~(60)Co γ-ray and the untreated material showed significant difference, then their zymogrames of the isoperoxidase appeared different. The progress of isoperoxidase of the leaf explants showed an obvious change in their successive differentiation. The pattern of isoperoxidase of the leaf explant cultured on the 3rd day in the differentiation medium showed an evident change, particularly at the region of slower migrating rate, some isozyme bands appeared, but they were not observed in the leaf itself. As the shoots developed, the pattern of isoperoxidase apparently changed. In the explant cultured on the 35th day the band of isozyme at the region of the slower migrating rate disappeared. There was no change in the zymograme patterns of the isoperoxidase between the irradiated explants with different dosags, and the CK during their progressive development of differentiation until the bud formation. In the mean time, however, the explants irradiated with different dosages were morphologically different in their differentiation, with no clear changes in their isoperoxidase patterns. The difference in the isozymes was observed only when the differentiated plantlets irradiated with ~(60)Co γ-ray grew in different conditions. From these results it seems that the mutagenic effects of ~(60)CO γ-ray on the development of isoperoxidase of the leaf of the treated plants or explants may be due to the interference of their levels of endogenous hormones. It has been shown that different concentrations or combinations of kinetin, coconut milk and 2,4-D might exert different effects on the growth and differentiation of the tissues. The patterns of isoperoxidase showed significent changes during the progressive dedifferentiation of the explant, but there was no change during bud formation as the explant was cultured on differentiation medium. The ~(60)Co γ-ray might exert certain regulatory effect on the level of the endogenous phytohormones which control growth and differentiation of the tissues. Further investigations are in progress.

在烟草的组织中,老叶的过氧化物酶,在迁移速率慢的酶区带对照植株比变异植株多二条酶带,但在幼龄叶片无差异。~(60)Coγ射线诱变的外植体与对照相比,酶谱有显著不同。在组织分化进程中,酶谱亦有显著变化。随着细胞分化进程,定期测定不同剂量γ射线处理的培养组织中酶谱的变化历程,观察到在分化前后酶谱无明显差异。只有在诱变植株生长状态表现明显差异时才见酶谱的变化。培养基中不同浓度组合的激动素、椰乳和2,4-D对细胞生长和分化有不同的调节作用,随着组织分化与脱分化历程,同工酶谱表现明显差异。在组织开始形成愈伤组织以后同工酶谱已有显著变化,但培养在分化培养基上的外植体,在分化前后,其同工酶谱未见明显变化。

1.The liquid medium and semi-solid semi-liquid medium were better

1.液体培养基与半固体半液体培养基在诱导愈组上优于固体培养基。半固体半液体培养基诱导愈组的能力与液体培养基相似,但从半固体半液体培养基中把愈组转移到分化培养基的操作工作则容易得多。2.由固体培养所得的愈组与由液体培养所得的、来自液体表面或沉入液体中的愈组,如早期转移,在分化幼苗的能力上没有明显区别。3.液体漂浮培养时,因培养基及杂交组合的不同,所得的愈组数目亦有明显差异,为获得较高的愈组诱导率,亦应选择不同杂交组合及采用不同培养基为宜。

 
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