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我国蜱
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  in ticks
     Isolation, Detection and Genotyping of Borrelia Burgdorferi Sensu Lato in Ticks from China
     我国蜱中莱姆病螺旋体的分离、检测和基因分型研究
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     Aim To search for the pathologic evidence of human granulocytc ehrlichia(HGE) infection in ticks.
     目的 寻找我国蜱中人粒细胞埃利希体感染存在的病原学证据。
短句来源
     Conclusion The findings further confirmed the dominance of B. garinii and B. afzelii in China. Coinfections of different genotypes are found at the first time in ticks collected from China.
     结论证实B.garinii和B.afzelii基因型为我国莱姆病螺旋体的优势基因型,并在我国蜱中发现莱姆病螺旋体不同基因(亚)型的混合感染。
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  “我国蜱”译为未确定词的双语例句
     Thus the S genes of these two strains were cloned, sequenced and compared with that of other CCHF virus strains. Results The result showed the nucleotides homology of the two S genes was 96.7%, the homology with other two XHFV isolated from tick(HY13) and sheep(C68031) in 1968 is high(96.7%~99%);
     结果 两株病毒核苷酸序列同源性为96 .7 % ,与已经发表的1968 年分离自我国蜱(HY13) 和羊(C68031) 的两株病毒核苷酸序列同源性均较高(96 .7 % ~99 % ) ;
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  相似匹配句对
     A NEW RECORD OF HAEMAPHYSALIS FROM CHINA——HAEMAPHYSALIS APONOMMOIDES
     我国一新纪录
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     IXODES (SCAPHIXODES) SEMENOVI OLENEV——A NEW RECORD OF CHINESE TICK FROM XIZANG, CHINA
     西氏硬我国的新纪录
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     China's OPAC
     我国的OPAC
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     WALNUT OF CHINA
     我国的核桃
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  in ticks
In addition, rickettsiae of spotted fever group (SFG), Anaplasma phagocytophilum and Borrelia burgdorferi sensu lato were found in ticks using genera or species-specific PCR methods.
      
Identification ofBorrelia in ticks by microscopic analysis, followed by a short-term treatment with antibiotics according to microbiological indications, is an efficient method for preventing persons from contracting borreliosis.
      
In ticks type 4 OspA has not been detected by culture so far.
      
However, as reported in a previous study, type 4 OspA could be detected in ticks by the highly sensitive PCR technique.
      
The results confirm that proteins of 22/23 kDa are differentially expressed duringin vitro subcultures and in ticks, and show that proteins which are not detectable afterin vitro culture may be reexpressed after reexposure ofB.
      
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This paper dealed with the descriptions of two species of Pygmephoroidea newly recorded from China: Brennandania lambi(Krczal, 1964) and Pseudopygmephorus incopspicuus(Berlese, 1904). The former species was collected from the bottles of mushroom spawn, the mushroom beds and the bottles of sitaki mushroom spawn contaminated by mould, the latter was from the mushroom beds. These two species are most common in mushroom cultivation in Shanghai, and B. lambi is the major pest causing the decrease of mushroom yield...

This paper dealed with the descriptions of two species of Pygmephoroidea newly recorded from China: Brennandania lambi(Krczal, 1964) and Pseudopygmephorus incopspicuus(Berlese, 1904). The former species was collected from the bottles of mushroom spawn, the mushroom beds and the bottles of sitaki mushroom spawn contaminated by mould, the latter was from the mushroom beds. These two species are most common in mushroom cultivation in Shanghai, and B. lambi is the major pest causing the decrease of mushroom yield greatly.

本文报道我国蜱螨目矮蒲螨总科的两个新记录种:兰氏布伦螨[Brennandania lambi (Krczal,1964)]和隐拟矮蒲螭[Pseudopygmephorus inconspicuus(Belese,1904)]。前者采于蘑菇菌种瓶、菇床和有杂菌污染的香菇菌种瓶;后者采于蘑菇菇床。二者均是上海蘑菇栽培业中最为常见的,其中兰氏布伦螨是严重影响蘑菇产量的关键性害螨。

Objective In recent years the antibody against Xinjiang hemorrhagic fever(XHF) was found in domestic animals or human sera in six provinces, which is a new public health problem. This paper is aimed to understand the essence of XHFV and reveal the relationship between the structure and the function. Methods The sequences of S gene from the virus isolated from the first Chinese case in 1965 and from a strain isolated from tick in 1984 have been analyzed. Thus the S genes of these two strains were cloned, sequenced...

Objective In recent years the antibody against Xinjiang hemorrhagic fever(XHF) was found in domestic animals or human sera in six provinces, which is a new public health problem. This paper is aimed to understand the essence of XHFV and reveal the relationship between the structure and the function. Methods The sequences of S gene from the virus isolated from the first Chinese case in 1965 and from a strain isolated from tick in 1984 have been analyzed. Thus the S genes of these two strains were cloned, sequenced and compared with that of other CCHF virus strains. Results The result showed the nucleotides homology of the two S genes was 96.7%, the homology with other two XHFV isolated from tick(HY13) and sheep(C68031) in 1968 is high(96.7%~99%); compared with S genes of other CCHFV, the homology was 77.4% to 92.7%. Phylogenetic tree showed that all Chinese isolates clustered into one branch which was further distributed into at least 3 main groups. Conclusion The results point out that the epidemic of the disease is originated from China.

目的 认识新疆出血热( XHF) 病毒不同分离株基因区别的本质,从分子水平揭示其基因结构与功能的关系,寻找传播及流行的原因。方法 对1965 年自我国首例病人及1984 年自蜱分离的两株XHF 病毒进行了S 基因片段的克隆测序和分析。结果 两株病毒核苷酸序列同源性为96 .7 % ,与已经发表的1968 年分离自我国蜱(HY13) 和羊(C68031) 的两株病毒核苷酸序列同源性均较高(96 .7 % ~99 % ) ;与其它CCHF 病毒相比S 基因同源性为77 .4 % ~92 .7 % 。结论 计算机绘出的系统发生树状图显示我国分离的4 株病毒形成一单个群体并进一步分为三组,提示流行源自我国。

Aim To search for the pathologic evidence of human granulocytc ehrlichia(HGE) infection in ticks.Method Specific primers derived from the 16S rRNA gene sequence were used to amplify ehrlichial DNA from ticks by hemi-nested polymerase chain reaction(PCR).The PCR products were cloned and sequenced.The sequence was compared with other ehrlichiae for homology.Results Specific DNA fragments were amplified from Ixodes persulcatus collected from Heilongjiang province.The minimum infection rate was 0.8%.The sequence...

Aim To search for the pathologic evidence of human granulocytc ehrlichia(HGE) infection in ticks.Method Specific primers derived from the 16S rRNA gene sequence were used to amplify ehrlichial DNA from ticks by hemi-nested polymerase chain reaction(PCR).The PCR products were cloned and sequenced.The sequence was compared with other ehrlichiae for homology.Results Specific DNA fragments were amplified from Ixodes persulcatus collected from Heilongjiang province.The minimum infection rate was 0.8%.The sequence of 919-bp PCR product was analyzed,and identified as part of ehrlichial 16S rRNA gene.It differed at four positions in comparison to American strains.Conclusion This is the first report of having HGE-like pathogen in China.These findings demonstrate that there may be natural foci of human granulocytic ehrlichiosis in northern forest area in China.

目的 寻找我国蜱中人粒细胞埃利希体感染存在的病原学证据。方法 应用从人粒细胞埃利希体 16SrRNA基因构建的特异引物进行半套式PCR ,检测蜱标本中埃利希体DNA。然后对PCR扩增产物进行克隆和序列测定 ,与已知序列进行同源性比较。结果 从黑龙江采集的全沟硬蜱 (Ixodespersulcatus)中扩增出特异DNA片段 ,计算最小阳性率为 0 8%。对 919bp的扩增产物进行序列分析 ,证实为埃利希体DNA ,与美国人粒细胞埃利希体分离株对应序列比较 ,相差 4个核苷酸。结论 这是首次证明我国有类似人粒细胞埃利希体的病原体存在。表明我国北方林区可能存在人粒细胞埃利希体感染的自然疫源地

 
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