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起始区
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  initial region
     Antisense phosphorothioate oligodeoxynueleotides respectively complementary to initial region of 2.1kb mRNA(oligomer A,14nts)and enhancer I(oligomer B,12nts)of HBV were synthesed by chemi cal synthetic method,and inhibition of these oligomers on expression of HBsAg and HBeAg in cell cul ture system was studied.
     设计合成了两个分别互补于乙肝病毒2.1kb mRNA起始区(片段A)和增强子区(片段B)的硫代磷酸的DNA片段。 在经克隆HBV DNA转染HepG_2细胞建立的HBV短暂表达系统及稳定产生HBV的2215细胞中研究二者对HBsAg及HBeAg表达的抑制作用。
短句来源
     The minor codons preferentially used in the initial region are thought to have a negative effect on gene expression,which can be explained by the minor codon modulator hypothesis.
     起始区的这种倾向性对冠状病毒基因的表达具有负性调控作用,可以用稀有密码子调控假说解释.
短句来源
  original region
     STRUCTURE ANALYSIS OF THE MITOCHONDRIAL tRNA~(Cys) GENE AND THE ORIGINAL REGION OF LIGHT-STRAND REPLICATION FROM CARP
     鲤鱼线粒体tRNA~(Cys)基因和轻链复制起始区的结构分析
短句来源
     Thecarp mitochondrial original region of light-strand replication consists of 36 baseswhich include 11 base pairs in its stemand 14 bases in its loop.
     鲤鱼线粒体L 链复制起始区含有36个碱基,复制起始区茎环结构中的茎含有11对碱基,而环则是由14个碱基组成。
短句来源
     By making acomparison between the nucleotide sequen-ces of the carp original region of light-strand replication and those of other 10vertebrates,it is further found that stemsequence of carp is very conservative whileloop sequense is very variable.
     同其它10种脊椎动物L-链复制起始区的核苷酸序列比较发现,鲤鱼茎环结构中的茎序列是非常保守的,而环的序列及环的长度则变化较大。
短句来源
  “起始区”译为未确定词的双语例句
     Ors12 DNA, the eukaryotic origin of DZA replication, was used as a DNA probe to identify the specific Ors12 DNA binding proteins in human serum.
     利用含有真核细胞复制起始区(OriginofDNAReplication)的Ors12DNA作为探针,检测了人血清中与Ors12DNA特异结合的蛋白质。
短句来源
     Objective: To obtain two different porcine zona pellucida proteins (pZP3β) in amino acid length, pZP3β161 and pZP3β263, and to further study whether the translation initiation region (TIR) effects expression efficiency of exogenous gene in E. coli.
     目的:探讨翻译起始区(translation initiation region,TIR)突变对两种氨基酸序列长度不同的猪卵透明带蛋白pZP3β161和pZP3β263在大肠杆菌中表达的影响。
短句来源
     The present study was designed to optimize the 5′ translation initiation region of human monocyte chemoattractant protein-1(MCP-1).
     本研究利用PCR技术将人单核细胞趋化蛋白-1基因(MCP-1)5′端翻译起始区进行优化改构。
短句来源
     Non-fused Expression of HAb18GEF by Reducing Stability of Translational Initiation Region in mRNA
     降低mRNA翻译起始区的稳定性原核非融合表达HAb18GEF
短句来源
     Sequence and Functional Analysis of the Chromosome Replication Origin ( oriC) of Streptoverticillum caespitosus ATCC27422
     头状链轮丝菌(Streptoverticillum caespitosus)ATCC27422染色体复制起始区(oriC)的序列分析与功能研究
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  相似匹配句对
     necking down region;
     颈缩 ;
短句来源
     The line density and area density have been counted.
     的密度。
短句来源
     Improvement of Expression Level by Increasing Number of Replication Origins in Piasmid
     增加复制起始对基因表达的影响
短句来源
     3. One point value of known thickness is only needed within entire computed region.
     3,整个计算只需一个起始点;
短句来源
     (2) stress beginning;
     (2)抓起始
短句来源
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  initial region
The concentration pulsations have a significant effect on the characteristics of the turbulent diffusion flame in its initial region.
      
In so doing, in the initial region behind the pipeline cross section in which the condition of gas-hydrate formation sets in, the process of deposition exhibits the former pattern.
      
The impact of parameters at the nozzle exit section on the characteristics of turbulence in the mixing layer in the initial region of jets is investigated.
      
The simulation of heat transfer and flow of generalized Newtonian liquid in the initial region of coaxial channel with arrhenius
      
The effect of dust particles on the film flow in the initial region and on the intensity of heat and mass transfer is demonstrated.
      
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  original region
In some cases, the accessions from a region of origin formed distinct clusters, suggesting that divergence has resulted in genetically distinct lines persisting in the original region of origin.
      
The analytical solution consists of employing suitable fictitious initial temperatures and fictitious extensions of the original region occupied by the melt.
      
Larger attempts at reforestation of grassland areas with this tree in the original region of occurrence have failed so far.
      
The article compares two approaches to continuation of solutions of external boundary-value problems for the Helmholtz equation outside the original region in the solution space - analytical continuation and the generalized image method.
      
The proposed method has certain advantages compared with the method that constructs the Green's function by conformally mapping the original region onto the unit disk.
      
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Based on the continuous change of the temperature of gas and particles, as well as treat of the heat transfer coeffecient, a mathematical model of transient heat transfer between suspending particles and hot gas with 0.1 Biot number is proposed. Its analytical solution gives the efficient time of heat transfer. By combining with the equations of particle accelerated motion in a vertical pipe, the efficient heat transfer height of the duct can also be determined.By the results of the theoretical analysis using...

Based on the continuous change of the temperature of gas and particles, as well as treat of the heat transfer coeffecient, a mathematical model of transient heat transfer between suspending particles and hot gas with 0.1 Biot number is proposed. Its analytical solution gives the efficient time of heat transfer. By combining with the equations of particle accelerated motion in a vertical pipe, the efficient heat transfer height of the duct can also be determined.By the results of the theoretical analysis using the mathematical model mentioned above, the experiments on the thermal model and the field test of a cement preheater, it is shown that the main heat transfer between the hot gas and the powdered cement raw material occurs in the beginning of the accelerated period of particles. The time required is not more than 0.03s, and the efficient heat transfer height is less than 200mm. So, the height of each duct should be shortened and the stages of a suspension preheater should be increased in order to improve its behaviour.

本文针对输送床颗粒加速段气固两相对流换热系数和温度二者均连续变化的实际,建立了Biot小于0.1时的群颗粒非稳态对流换热数学模型,可求解气固有效换热时间,结合颗粒运动方程,能确定出有效换热管的高度。 数学模型的理论计算、热模试验及现场实测均表明,水泥生料粉和高温气流的换热主要发生在加速段的起始区,其距离不超过20cm,时间不大于0.03s。因而指出降低各级预热器输送管的长度、增加预热器的级数是提高热效率的重要途径。

Using rat AFP cDNA clone p~(RAF)87 andsubcloned 5' end fragment of AFP gene asprobes,we have analysed the DNaseI sensi-tivity and DNaseI hypersensitive sites ofAFP gene in chromatin of rat fetal liver(active transcription),adult liver (potentiallyactive transcription) and adult kidney (inac-tive transcription).The results indicatedthat fetal liver AFP genes are much moresensitive than their counter parts.There arethree DNaseI hypersensitive sites in the fetalAFP gene,namely,upstream-3kb region,1.37kb region...

Using rat AFP cDNA clone p~(RAF)87 andsubcloned 5' end fragment of AFP gene asprobes,we have analysed the DNaseI sensi-tivity and DNaseI hypersensitive sites ofAFP gene in chromatin of rat fetal liver(active transcription),adult liver (potentiallyactive transcription) and adult kidney (inac-tive transcription).The results indicatedthat fetal liver AFP genes are much moresensitive than their counter parts.There arethree DNaseI hypersensitive sites in the fetalAFP gene,namely,upstream-3kb region,1.37kb region just arround transcription startsites and the last located in downstream 4.1kb EcoR Ⅰ fragment.The 1.37 kb DNase Ⅰhypersensitive site also exists in adult liver.The relationship between AFP gene DNase Ⅰsensitivity,DNase Ⅰ hypersensitive sites andAFP gene expression have been discussed.

我们用大鼠AFP基因cDNA pRAF 87和5'端基因克隆片段为探针,分别探测了AFP基因活跃表达的胚肝组织、具表达潜能的成年肝组织以及不表达的肾组织AFP基因转录区和调控区的DNase Ⅰ敏感性和超敏感区。结果表明;胚肝组织和成年肝组织AFP基因在转录区和5'端调控区都显示出较高的DNase Ⅰ敏感性,而肾组织则不敏感。胚肝组织AFP基因存在三个DNase Ⅰ超敏感区:基因转录起始区,离转录起始区上游约-3 kb左右区域,另一超敏感区位于基因内部4.1 kb EcoR Ⅰ片段内。成年肝组织在转录起始区也存在一超敏感区,在基因内部4.1 kb EcoR Ⅰ片段显示较高的DNase Ⅰ敏感性。推测AFP基因上游-3 kb区域(1.5 kb HindⅢ片段区)DNase Ⅰ超敏感区可能与增强基因转录活性相关,而转录起始区超敏感区可能是维持细胞组织专一性表达所必需,基因转录区域内部DNase Ⅰ超敏感区可能与AFP基因维持具活跃表达或潜能表达的染色质空间结构相关。

In this paper,by means of PCR amplification,site-specific mutagenesis,DNA recombination in vitro and optimization of translation initiation,we constructed a recombinant plasmid pBV-IL-6 that overproduces biologically active human interleukin-6(rhIL-6)in E.coli.The expressed rhIL-6 lacks the first 25 amino acids of mature hIL-6 and accounts for 71% of the total bacteria proteins.The reasons why a deleted rather than mature IL-6 was chosen for expression in E.coli are as follows:1)The specific bio-activities of...

In this paper,by means of PCR amplification,site-specific mutagenesis,DNA recombination in vitro and optimization of translation initiation,we constructed a recombinant plasmid pBV-IL-6 that overproduces biologically active human interleukin-6(rhIL-6)in E.coli.The expressed rhIL-6 lacks the first 25 amino acids of mature hIL-6 and accounts for 71% of the total bacteria proteins.The reasons why a deleted rather than mature IL-6 was chosen for expression in E.coli are as follows:1)The specific bio-activities of the deleted hIL-6 are the same as,even more than that of the mature form according to the published data;2)The deleted part of IL-6 coding region is rich in C-G and

本研究通过计算机分析设计、人工合成的寡核苷酸引物进行定点诱变和转译起始区优化,再经 PCR 扩增和体外重组,获得重组质粒 pBV-IL-6,转化大肠杆菌获得重组人白细胞介素6的工程菌 EcoliJM103/pBV-IL-6.结果,表达 IL-6占菌体总蛋白的71%.经 IL-6依赖的小鼠杂交瘤细胞系7TDI 和~(?)H-TdR 掺入法测定表达产物粗提物的 IL-6生物活性为10~6U/L,经凝胶过滤纯化与复性之后 IL-6比活性为10~(?)U/mg.表达产物为缺失 N 端25个氨基酸的人白细胞介素6.

 
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