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彗星分析
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  comet assay
     Then the tumor cells irradiated to a dose of 600cGy underwent neutral comet assay at 1h, 2h, 4h, and 8h after irradiation, and their residue DNA double strands breaks (DSBs) at these time points were determined respectively with the tail moment,and compared with the results of clonogenic assay.
     然后进行两种细胞于600cGy照射后1、2、4、8h的中性彗星分析,以尾力矩为指标,动态检测不同时间点残留的DNA双链断裂,并与克隆形成分析结果相比较。
短句来源
     These techniques possess the characters of simplicity,reproducibility and sensitivity,compared with classical methods such as comet assay and micronucleus test.
     这些技术与检测基因突变、染色体畸变和损伤为主的一系列经典研究方法如彗星分析、微核实验等相比具有简便、快速、灵敏等优点。
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  “彗星分析”译为未确定词的双语例句
     Oliver tail moment was used to evaluate DNA damage of cells from the mice using an IMI comet analysis microsoft.
     IMI彗星分析软件分析彗星尾距(oliver tail moment,TM)评价细胞DNA损伤的程度。
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     Objective: In order to understand the relationship between DNA damage and hepatocellular carcinoma (HCC), and the tanshinone (丹参酮 TAN) protection for this damage. Methods : We detected DNA in peripheral blood lymphocytes of three groups in healthy people, in patients with chronic hepatitis B and chronic hepatitis B complicated by HCC. In the group of patients with chronic hepatitis B we did treatment of both TAN and H2O2 in vitro.
     为探讨乙型肝炎病毒引起DNA损伤与肝癌形成的关系,以及丹参酮对DNA的保护作用,本课题使用单细胞凝胶电泳(SCGE)技术以及IMI 1.0彗星分析软件在体外观察健康组、慢性乙型肝炎组、慢性乙型肝炎合并肝癌组及慢性乙型肝炎组经丹参酮、H_2O_2处理后外周血淋巴细胞DNA的彗星状荧光图像。
短句来源
     A novel fast multispectral image analyzing system of single cell gel electrophoresis is developed. With the features of having Chinese-English exchange interface and an auto-analyzing system,the system can obtain parameters about comet,comet tail and comet head simultaneously,and perform analysis more conveniently and faster with better accuracy,The application of the system for analyzing the effect of H 2 O 2 -induced DNA damage in lymphocytes proves that the system can efficiently meet the needs of SCGE analysis.
     设计了一套多光谱单细胞凝胶电泳(SCGE)图像分析系统,利用该系统能简便、快捷、准确地同时获得单细胞彗星图像包括彗星、彗星尾、彗星头参数的多个彗星分析指标。 并使用该系统测量了H2O2致淋巴细胞DNA损伤的作用,实验证明,该系统可较好的满足SCGE图像的分析要求。
短句来源
     After incubation at: different conditions, single cell electrophoresis (SCGE)-comet assay was performed for observation of DNA damage under fluorescent microscope and the photo pictures.
     将上述作用后的淋巴细胞进行单细胞凝胶电泳(single-cell gel electrophoresis)又称彗星试验(cometassay)处理,获取的胶板置于荧光显微镜下观察、摄取图像,再用本校生命科学院的IMI1.0彗星分析软件以“彗星”尾长、尾%DNA、尾惯量和尾矩来评价淋巴细胞DNA损伤程度。
  相似匹配句对
     The encryption and weaknesses of the E. ?
     分析了E.
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     , G.
     分析,G.
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     L' IDENTIFICATION DES SPECTRES DE COMèTES ET L' ANALYSE DES SPECTRES DE L' AMMONIAC
     彗星光谱的证认与氨的光谱的分析
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  comet assay
The single cell gel electrophoresis or comet assay was used to quantify DNA strand breaks as a measure of DNA damage induced by Cd and imidacloprid contamination in soil.
      
Roots ofVicia faba were exposed to the contaminated soil for 2 h at 25°C and were used in the comet assay.
      
By means of comet assay, a study of kinetics curve of DNA damage repair in irradiated SX-9 cells that came from mouse breast cancer proceeded.
      
Using the alkaline comet assay, we showed that antioxidants - vitamins C and E, quercetin, and melatonin - reduced the genotoxic effect of MNNG in H.
      
Carotenoid concentrations of lymphocytes were determined by HPLC and DNA damage was evaluated by the comet assay following an ex vivo treatment with H2O2.
      
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PURPOSE To study the possibility of using the comet assay for detection of radiation induced DNA damage and repair in NPC cells.METHODS Using the alkaline comet assay,a microelectrophoretic technique for detection of visual DNA single strand breaks(SSBs)was used to quantify the DNA SSBs,the correlation between DNA damage and the radiation dose in both of human well and poorly differentiated NPC cell lines(CNE 1 and CNE 2Z)after X ray radiation was given.Also to determine their spontaneous DNA repair of...

PURPOSE To study the possibility of using the comet assay for detection of radiation induced DNA damage and repair in NPC cells.METHODS Using the alkaline comet assay,a microelectrophoretic technique for detection of visual DNA single strand breaks(SSBs)was used to quantify the DNA SSBs,the correlation between DNA damage and the radiation dose in both of human well and poorly differentiated NPC cell lines(CNE 1 and CNE 2Z)after X ray radiation was given.Also to determine their spontaneous DNA repair of SSB after exposure to x ray. RESULTS The amount of DNA SSBs induced by a 2?Gy radiation single dose was definitely detected in both cell lines.This assay presented the different results of DNA damage induced by different radiation doses;their DNA damage was increased following the increase of the doses;and there was indeed a good linear relationship between the amout of damage detected with this assay and the dose of radiation given to the cells.The repair of 15?Gy radiation induced DNA damage in both of cell line was definitely quantified by detection of the amount of damage at 10 min after exposure to radiation;also the amount of damage was reduced quickly to under half the level of the initial damage within 30?min. Then the repair of DNA damage became slow,and continue for about 2 h to reach the level of unirradiated cells.CONCLUSIONS Using the alkaline comet assay,the fine results of detection can exactly represent the amount of DNA damage induced by radiation in two human NPC cell lines,and the correlation between the amount of DNA damage and the dose of radiation;also the quantitative change for the ability of the spontaneous DNA repair during the period of detetion.

目的 探讨“彗星”分析法(CometAssay)检测鼻咽癌细胞DNA放射与修复反应的可能性。 方法 采用碱性“彗星”分析法的微胶电泳技术,定量检测评价人高、低分化鼻咽癌细胞系CNE1和CNE2Z细胞在接受X射线照射后,其DNA单链断裂(SingleStrandBreaks,SSBs)的程度,及其与放射剂量的关系和DNA的SSBs在不同检测时间的自身修复情况。 结果 用碱性“彗星”分析法可准确地检测出2Gy单一放射剂量所诱导的两系细胞DNA的SSBs;可见DNA损伤程度在不同照射剂量其结果不同,随剂量递增DNA的损伤加重,与放射剂量呈良好线性相关。15Gy诱导两系细胞的DNA损伤后,10min即可定量检测出受损DNA的确切恢复变化,在照射30min内,其修复较迅速,其损伤程度可降至初时的一半以下,其后修复缓慢,约需持续2h才能复至无照射细胞水平。 结论 “彗星”分析法检测结果能准确反映人鼻咽癌细胞DNA的定量损伤程度及其与放射剂量的关系,以及损伤后自身修复能力在受测时间中的定量变化情况

Objective To establish the relationship between radiosensitivity and radiation-induced DNA damage and repair in four human tumor cell lines by means of comet assay. Methods With the alkaline comet assay,the amounts of DNA single strand breaks (SSB) were quantified. The correlation between a certain DNA damage and the relevant radiation dose was investigated in four human tumor cell lines: the well differentiated nasopharyngeal squamous carcinoma cell line (CNE-1), the esophageal squamous carcinoma cell line...

Objective To establish the relationship between radiosensitivity and radiation-induced DNA damage and repair in four human tumor cell lines by means of comet assay. Methods With the alkaline comet assay,the amounts of DNA single strand breaks (SSB) were quantified. The correlation between a certain DNA damage and the relevant radiation dose was investigated in four human tumor cell lines: the well differentiated nasopharyngeal squamous carcinoma cell line (CNE-1), the esophageal squamous carcinoma cell line (Ec-109), the lung adenocarcinoma cell line (GLC), and the poorly differentiated gastric adenocarcinoma cell line (BGC-823) ,which had been exposed to 6 MV X-ray. In our study, the spontaneous DNA repair of SSB after exposure was also determined for cell lines of CNE-1 and GLC, and the result was compared to that from the clonogenic cell surviving assay.Results The amount of DNA SSB induced by irradiation of a single dose of 16 Gy was definitely detected with the alkaline comet assay in all cell lines. It was found that there was a close association of DNA damage and the delivered dose; that is the larger the dose, the severer the DNA SSB. For the same single dose of 16 Gy according to a descending order, the different DNA damages ranked as: CNE-1>Ec-109>GLC>BGC-823. The Do values of CNE-1, Ec-109, GLC and BGC-823 were 1.25, 1.27, 1.88 and 1.56, respectively. The half repair time was 17.8?min for CNE-1 and 7.4?min for GLC..Conclusions It is concluded that this assay definitely detects, and presents exactly the amount of DNA damage induced by radiation in four human tumor cell lines (CNE-1, Ec-109, GLC and BGC-823), and quantitatively demonstrates the spontaneous repair of DNA damage as well.The radiosensitivity,with different Do values for squamous carcinoma or adenocarcinoma cell lines,is correlative to the ability of DNA-damage repair.

探讨“彗星”分析法 (CA)在检测体外培养人肿瘤细胞DNA放射损伤与修复和放射敏感性的关系。方法 采用碱性CA检测鼻咽高分化鳞癌上皮细胞系 (CNE 1)、食管鳞癌上皮细胞系 (Ec 10 9)、肺腺癌细胞系 (GLC)和胃低分化腺癌细胞系 (BGC 82 3)经 6MVX射线照射后单细胞内DNA单链断裂 (SSB)程度及其与放射剂量的关系 ,以及CNE 1和GLC细胞系在分别接受了 16GyX射线照射后的自身修复情况 ,同时与相应的细胞存活曲线比较。结果 碱性CA可以检测出 4个细胞系DNA的SSB ,并与放射剂量呈良好的线性关系。 16Gy剂量下各细胞系的SSB程度依次为CNE 1>Ec 10 9>GLC >BGC 82 3。CNE 1,Ec 10 9,GLC和BGC 82 3细胞系的Do值分别为 1.2 5 ,1.2 7,1.88和 1.5 6。 16Gy诱导CNE 1和GLC细胞系的DNA损伤后 ,其半修复时间分别为 7.4,17.8min。结论 采用碱性CA能确切反映 4种人肿瘤细胞系DNA的定量损伤程度及其与放射剂量的关系 ,并能确切反映辐射损伤后自身修复能力 (CNE 1和GLC)在受测时间中的...

探讨“彗星”分析法 (CA)在检测体外培养人肿瘤细胞DNA放射损伤与修复和放射敏感性的关系。方法 采用碱性CA检测鼻咽高分化鳞癌上皮细胞系 (CNE 1)、食管鳞癌上皮细胞系 (Ec 10 9)、肺腺癌细胞系 (GLC)和胃低分化腺癌细胞系 (BGC 82 3)经 6MVX射线照射后单细胞内DNA单链断裂 (SSB)程度及其与放射剂量的关系 ,以及CNE 1和GLC细胞系在分别接受了 16GyX射线照射后的自身修复情况 ,同时与相应的细胞存活曲线比较。结果 碱性CA可以检测出 4个细胞系DNA的SSB ,并与放射剂量呈良好的线性关系。 16Gy剂量下各细胞系的SSB程度依次为CNE 1>Ec 10 9>GLC >BGC 82 3。CNE 1,Ec 10 9,GLC和BGC 82 3细胞系的Do值分别为 1.2 5 ,1.2 7,1.88和 1.5 6。 16Gy诱导CNE 1和GLC细胞系的DNA损伤后 ,其半修复时间分别为 7.4,17.8min。结论 采用碱性CA能确切反映 4种人肿瘤细胞系DNA的定量损伤程度及其与放射剂量的关系 ,并能确切反映辐射损伤后自身修复能力 (CNE 1和GLC)在受测时间中的定量变化情况。自身修复能力的大小在鳞癌和腺癌细胞系间与放射敏感性的大小 (Do值 )有较好的对应关系。

SUMMARY Objective: To determine the potency of QN 2013, a derivative of quinoxaline 1,4 N oxide, as a hypoxia selective cytotoxin or a radiosensitizer. Methods: In vitro cytotoxicity and radiosensitization, as well as in vivo antitumor activity were determined by colony formation and tumor growth delay respectively. The changes in the cell cycle, DNA damage and repair of damaged DNA were assayed by FCM and “comet” assay, separately. Results: IC N2 50 and IC air 50 of QN 2013 for...

SUMMARY Objective: To determine the potency of QN 2013, a derivative of quinoxaline 1,4 N oxide, as a hypoxia selective cytotoxin or a radiosensitizer. Methods: In vitro cytotoxicity and radiosensitization, as well as in vivo antitumor activity were determined by colony formation and tumor growth delay respectively. The changes in the cell cycle, DNA damage and repair of damaged DNA were assayed by FCM and “comet” assay, separately. Results: IC N2 50 and IC air 50 of QN 2013 for HeLa S3 cells were 0.08 and 1.7 mmol·L -1 respectively, namely, HCR=21. This suggested that QN 2013 was a fairly hypoxic cytotoxin, but inferior to SR 4233. QN 2013 had an evident radiosensitization either in vitro or in vivo . It was noted, however, that the value of in vitro SERs increased exponentially with increasing concentration of the drug, but the in situ antitumor activity seemed to be independent of doses of the drug. The systemic toxicity of QN 2013 was superior to an LD 50 of 265 mg·kg -1 compared with 80 mg·kg -1 for SR 4233. In hypoxic condition QN 2013 induced S retension effect and G 2M block in HeLa S3 cells, caused DNA double strand break, and inhibited the repair of radiation induced DNA damages. All of these reactivenesses might be involved in the action mechanism of QN 2013. Conclusion: QN 2013 is a fair hypoxia selective cytotoxin, and has shown improved antitumor activity in vivo in combination with radiation. In general, These results suggest that the series of quinoxaline di N oxide derivatives hold out bright prospect for the development of novel bioreductive antitumor drugs.

目的 :检验和评价喹喔啉双氮氧化物衍生物QN 2 0 13的选择性乏氧细胞毒性和放射增敏作用。方法 :体外细胞毒性、放射增敏作用及体内抗肿瘤活性 ,分别用集落形成法和肿瘤生长延迟法检验。细胞周期的变化、DNA损伤和损伤DNA的修复分别以流式细胞术和“彗星”分析法测定。结果 :QN 2 0 13对乏氧和富氧HeLa S3细胞的等毒性浓度ICN250 和ICair50 分别是 0 .0 8和 1.7mmol·L-1,乏氧细胞毒性比率 (hypoxiccytotoxicityratio ,HCR)为 2 1。这说明QN 2 0 13具有一定的乏氧细胞毒性 ,但弱于代表性生物还原药SR 42 33。在 1mmol·L-1以下时 ,QN 2 0 13的体外放射增敏作用比MISO(misonidazole)弱 ,但随药物浓度增加近似按指数增大 ,而荷瘤小鼠腹腔给药则体内增敏作用与施药剂量无明显依赖关系。在细胞和分子水平上 ,QN 2 0 13诱发乏氧HeLa S3细胞G2 M期阻断 ,引起DNA双链断裂 ,且抑制辐射DNA损伤的修复。结论 :QN 2 0 13是一中度活性的乏氧选择性细胞毒剂 ,体内外实验表明具有一定的放...

目的 :检验和评价喹喔啉双氮氧化物衍生物QN 2 0 13的选择性乏氧细胞毒性和放射增敏作用。方法 :体外细胞毒性、放射增敏作用及体内抗肿瘤活性 ,分别用集落形成法和肿瘤生长延迟法检验。细胞周期的变化、DNA损伤和损伤DNA的修复分别以流式细胞术和“彗星”分析法测定。结果 :QN 2 0 13对乏氧和富氧HeLa S3细胞的等毒性浓度ICN250 和ICair50 分别是 0 .0 8和 1.7mmol·L-1,乏氧细胞毒性比率 (hypoxiccytotoxicityratio ,HCR)为 2 1。这说明QN 2 0 13具有一定的乏氧细胞毒性 ,但弱于代表性生物还原药SR 42 33。在 1mmol·L-1以下时 ,QN 2 0 13的体外放射增敏作用比MISO(misonidazole)弱 ,但随药物浓度增加近似按指数增大 ,而荷瘤小鼠腹腔给药则体内增敏作用与施药剂量无明显依赖关系。在细胞和分子水平上 ,QN 2 0 13诱发乏氧HeLa S3细胞G2 M期阻断 ,引起DNA双链断裂 ,且抑制辐射DNA损伤的修复。结论 :QN 2 0 13是一中度活性的乏氧选择性细胞毒剂 ,体内外实验表明具有一定的放射增敏作用 ,明显增强辐射抗肿瘤能力。其作用机制可能是通过直接损伤DNA和抑制DNA修复。这些结果提示虽QN 2 0 13本身可能不是理想的生物还原药 ,但喹喔啉氮氧化物系列不失为探索新抗癌药应重视的领域之一。

 
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