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彗星分析
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  “彗星分析”译为未确定词的双语例句
    Oliver tail moment was used to evaluate DNA damage of cells from the mice using an IMI comet analysis microsoft.
    IMI彗星分析软件分析彗星尾距(oliver tail moment,TM)评价细胞DNA损伤的程度。
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  comet assay
The single cell gel electrophoresis or comet assay was used to quantify DNA strand breaks as a measure of DNA damage induced by Cd and imidacloprid contamination in soil.
      
Roots ofVicia faba were exposed to the contaminated soil for 2 h at 25°C and were used in the comet assay.
      
By means of comet assay, a study of kinetics curve of DNA damage repair in irradiated SX-9 cells that came from mouse breast cancer proceeded.
      
Using the alkaline comet assay, we showed that antioxidants - vitamins C and E, quercetin, and melatonin - reduced the genotoxic effect of MNNG in H.
      
Carotenoid concentrations of lymphocytes were determined by HPLC and DNA damage was evaluated by the comet assay following an ex vivo treatment with H2O2.
      
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Purpose: To study DNA damages induced by 60 Co γ _r adiation and o_phenylenediamine(o_PDA) using comet assay. Methods: The DNA damages induced by 0, 2, 4, 6, 8, 10, 15 Gy 60 Co γ_rays and 0, 2, 4, 6, 8 μmol / L o_PDA were measured using comet assay. The dose_respose relationship of the CHL cell t ail length and the dose of the two type of mutagen were established with Origin 4 software. Results: The tail length o f comet, index of DNA damage in this experiment, increased with the dose of 60 Co...

Purpose: To study DNA damages induced by 60 Co γ _r adiation and o_phenylenediamine(o_PDA) using comet assay. Methods: The DNA damages induced by 0, 2, 4, 6, 8, 10, 15 Gy 60 Co γ_rays and 0, 2, 4, 6, 8 μmol / L o_PDA were measured using comet assay. The dose_respose relationship of the CHL cell t ail length and the dose of the two type of mutagen were established with Origin 4 software. Results: The tail length o f comet, index of DNA damage in this experiment, increased with the dose of 60 Co γ_ray and concentration of o_PDA, resp ectively. The dose_respose relationship of the CHL cell tail l ength(TL)and the dose of 60 Co γ_ray fitted for linear_quadratic model: TL=20.41+2.42D+0.38D 2; The dose_response relationships fo r DNA damage induced by o_PDA fitted for linear_quadratic mode l: TL=1.90+1.46C+0.52C 2. Conclusion: The comet assay is a use ful tool for determining the genotoxicity of environmental agent s.

目的 :用彗星分析技术(cometassay)检测邻苯二胺(o_phenylenediamine,o_PDA)和 60Coγ_射线对CHL细胞DNA的损伤。方法 :CHL细胞经0、2、4、6、8μmol/L的邻苯二胺染毒和0、2、4、6、8、10、15Gy的 60Coγ_射线照射后 ,进行单细胞凝胶电泳 ,测定彗星尾长。采用Origin4.2软件建立两种诱变剂的剂量与CHL细胞彗星尾长的剂效关系。 结果 :DNA的损伤程度(以彗星尾长为指标)随 60Coγ_射线剂量的加大和o_PDA浓度的增加而增强 ,CHL细胞彗星尾长(TL)与 60Coγ_射线的剂效关系符合线性平方模型 :TL=20.41 +2.42D +0.38D2;与o_PDA浓度的剂效关系符合线性平方模型 :TL=1.90 +1.46C +0.52C2。 结论 :60Coγ_射线剂量和o_PDA的浓度与细胞DNA损伤程度具有剂效关系 ;彗星分析是一种分析环境危害因子遗传毒性的有效技术

Objective To further study the effects of benzene exposure on chromosome aberrations and DNA damages of the peripheral blood cells of the benzene-exposed workers. Methods Measure the benzene concentration with 3M passive dosimetry badges. Analyze chromosome aberrations of peripheral lymphocytes using non-banding method and determine DNA damages with the Trevigen TM Comet Kit.Results Numerically aberrated cell rate of the benzene-exposed workers [13.00%(2.50%~21.00%)]was significantly higher than that...

Objective To further study the effects of benzene exposure on chromosome aberrations and DNA damages of the peripheral blood cells of the benzene-exposed workers. Methods Measure the benzene concentration with 3M passive dosimetry badges. Analyze chromosome aberrations of peripheral lymphocytes using non-banding method and determine DNA damages with the Trevigen TM Comet Kit.Results Numerically aberrated cell rate of the benzene-exposed workers [13.00%(2.50%~21.00%)]was significantly higher than that of the controls [10.50%(3.50%~18.00%),P<0.05]; Aberrated cell rate (excluded gaps) of the benzene-exposed workers [14.50%(5.00%~23.50%)] was significantly higher than that of the controls [11.75(3.50~18.00), P<0.05]; Olive moment of the benzene-exposed workers [7.42(2.79~26.36)] was also significantly higher than that of the controls [3.30(1.49~10.37),P<0.001];There were dose-effect relationships between benzene exposure and hyperploidy cell rate, numerically aberrated cell rate, aberrated cell rate (excluded gaps) and olive moment.Conclusion Benzene exposure resulted in an increase of chromosome aberrations and DNA damages of the peripheral blood cells, which was in the dose-effect relationship manner.

目的 研究苯接触对工人外周血细胞染色体畸变及DNA的影响。方法 以个体采样器测定苯浓度 ,以非分带染色的方法分析外周血淋巴细胞染色体畸变 ,以TrevigenTM 彗星分析试剂盒测定外周血白细胞DNA损伤。结果 苯接触组的数目畸变细胞率 [13 0 0 % ( 2 5 0 %~ 2 1 0 0 % ) ]显著高于对照组 [10 5 0 % ( 3 5 0 %~ 18 0 0 % ) ,P <0 0 5 ] ,畸变细胞率 [不包括裂隙 ,14 5 0 % ( 5 0 0 %~ 2 3 5 0 % ) ]显著高于对照组 [11 75 ( 3 5 0~ 18 0 0 ) ,P <0 0 5 ] ,Olive尾矩 [7 42 ( 2 79~2 6 3 6) ]也显著高于对照组 [3 3 0 ( 1 49~ 10 3 7) ,P <0 0 0 1] ,超二倍体细胞率、数目畸变细胞率、畸变细胞率 (不包括裂隙 )及Olive尾矩均与苯接触呈剂量 效应关系。结论 苯接触导致外周血细胞染色体畸变及DNA损伤增加 ,且呈剂量 效应关系

BACKGROUND&AIM: To study the oxidative damage and genotoxicity induced by3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone(MX)in mice,and to explore the possible mechanism of genotoxicity caused by MX. MATERIAL AND METHODS: Mice were divided into four groups including solvent control group and three MX-treated groups(11,33,100mg/kg).Test substances were administered intraperitoneal(i.p.)and mice were sacrificed3hours after the treatment.DNA damage and malondialdehyde(MDA)in livers,kidneys and small intestines...

BACKGROUND&AIM: To study the oxidative damage and genotoxicity induced by3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone(MX)in mice,and to explore the possible mechanism of genotoxicity caused by MX. MATERIAL AND METHODS: Mice were divided into four groups including solvent control group and three MX-treated groups(11,33,100mg/kg).Test substances were administered intraperitoneal(i.p.)and mice were sacrificed3hours after the treatment.DNA damage and malondialdehyde(MDA)in livers,kidneys and small intestines were examined using the alkaline single-cell gel electrophoresis(SCGE,comet assay)and test kit. RESULTS: It was observed that MX yielded significant increase in the DNA damage in small intestines of mice at all dosage and in liver and kidney of mice at higher dosage compared with the solvent control(P<0.01).The concentration of MDA in livers,kidneys and small intestines of mice in the highest MX-exposed groups were significantly higher than that in solvent control group(P<0.05).There was a significant correlation between MDA and Olive tail moment in liver,kidney and small intestines. CONCLUSION: MX could induce obvious DNA damage and oxidative stress in multiple organs of mice.The cell oxidative damage might be one of the primary mechanisms of genotoxicity of MX.

背景与目的 :研究饮水氯化消毒副产物3_氯_4_二氯甲基_5_羟基_2(5氢)_呋喃酮(3_chloro_4_(dichloromethyl)_5_hydroxy_2[5H]_furanone,MX)对小鼠的遗传毒性与氧化损伤的诱导。 材料与方法 :昆明种小鼠随机分为4组 ,即溶剂对照组与低、中、高3个MX剂量组(11、33、100mg/kg) ,受试小鼠经腹腔注射染毒3h后处死。应用单细胞凝胶电泳技术(SCGE ,彗星分析)检测小鼠肝、肾、小肠的DNA损伤 ,并测定小鼠肝、肾和小肠中脂质过氧化主要终产物丙二醛(Malondialdehyde,MDA)的含量。结果 :随着MX浓度的增加 ,小鼠肝、肾、小肠中MDA含量与其细胞的Olive尾矩明显升高 ,且呈现较好的剂量依赖性。与溶剂对照组比较 :①MX各剂量组小肠细胞Olive尾距均显著性增加 ,肝、肾细胞的Olive尾距在MX较高剂量时有显著性增加 ;②中、高剂量组小鼠肝组织中MDA含量显著性升高 ,高剂量组小鼠肾和小肠组织中MDA含量明显升高 ,差异有显著性意义。肝、肾、小肠MDA含量与Olive尾矩均呈明显正相关。结论 :MX可引发哺乳动物多...

背景与目的 :研究饮水氯化消毒副产物3_氯_4_二氯甲基_5_羟基_2(5氢)_呋喃酮(3_chloro_4_(dichloromethyl)_5_hydroxy_2[5H]_furanone,MX)对小鼠的遗传毒性与氧化损伤的诱导。 材料与方法 :昆明种小鼠随机分为4组 ,即溶剂对照组与低、中、高3个MX剂量组(11、33、100mg/kg) ,受试小鼠经腹腔注射染毒3h后处死。应用单细胞凝胶电泳技术(SCGE ,彗星分析)检测小鼠肝、肾、小肠的DNA损伤 ,并测定小鼠肝、肾和小肠中脂质过氧化主要终产物丙二醛(Malondialdehyde,MDA)的含量。结果 :随着MX浓度的增加 ,小鼠肝、肾、小肠中MDA含量与其细胞的Olive尾矩明显升高 ,且呈现较好的剂量依赖性。与溶剂对照组比较 :①MX各剂量组小肠细胞Olive尾距均显著性增加 ,肝、肾细胞的Olive尾距在MX较高剂量时有显著性增加 ;②中、高剂量组小鼠肝组织中MDA含量显著性升高 ,高剂量组小鼠肾和小肠组织中MDA含量明显升高 ,差异有显著性意义。肝、肾、小肠MDA含量与Olive尾矩均呈明显正相关。结论 :MX可引发哺乳动物多种脏器的脂质过氧化反应增强 ;并能引起细胞DNA损伤。MX导致机体组织的氧化损伤可能在细胞遗传毒性作用中起重要作用。

 
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