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大鼠原位肝移植     
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  rat orthotopic liver transplantation
     Objective To increase the stability of rat orthotopic liver transplantation models effectively by improving the surgical procedures.
     目的通过对二袖套法大鼠原位肝移植手术方法的改进,提高大鼠肝移植动物模型的稳定性。
短句来源
     Comparison of three rat orthotopic liver transplantation models with 150transplants
     150例大鼠原位肝移植手术方法比较
短句来源
     Expression of chemotactic factor macrophage inflammatory protein 2 following rat orthotopic liver transplantation
     巨噬细胞炎性蛋白-2在大鼠原位肝移植后的表达
短句来源
     Establishment and improvement of rat orthotopic liver transplantation model with two-cuff technique
     两套袖法大鼠原位肝移植模型的建立及改进
短句来源
     Results Three hundred sixty cases of rat orthotopic liver transplantation were performed, and the successful rate of the mode was 91.3%.
     结果 共施行大鼠原位肝移植 36 0次 ,手术成功率为 91 3%。
短句来源
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  orthotopic liver transplantation in rats
     Technique of orthotopic liver transplantation in rats
     大鼠原位肝移植模型的手术操作技巧探讨
短句来源
     Orthotopic Liver Transplantation in Rats and Observations of the Immune Response
     大鼠原位肝移植模型的建立及高免疫应答鼠种的选择
短句来源
     Method of Orthotopic Liver Transplantation in Rats
     大鼠原位肝移植动物模型制作要点
短句来源
     Role of Kupffer Cells in the Immunoregulation of Orthotopic Liver Transplantation in Rats
     Kupffer细胞在大鼠原位肝移植免疫调节中的作用
短句来源
     Objective: To establish the model of arterialized two culf method for orthotopic liver transplantation in rats and evaluate the role of it.
     目的:建立动脉化双袖套法大鼠原位肝移植模型制作方法并对动脉化大鼠原位肝移植模型的地位进行探讨。
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  orthotopic liver transplantation in rat
     (2) to build experimental model of orthotopic liver transplantation in rat (ROLT), summarize operative skills and technique, observe the development process and basic pathophysiologic changes of acute rejection (AR) following allotransplantation from Lewis (LEW) to Brown Norway (BN) rat;
     (2)建立大鼠原位肝移植动物模型,总结手术技巧和方法,观察 Lewis(Lew)到 Brown Norway(BN)大鼠同种异体急性排斥反应(acute rejection, AR)的发生发展以及基本病理生理指标变化过程。
短句来源
     Some experience of surgical skills in the model of orthotopic liver transplantation in rat
     大鼠原位肝移植模型的手术技巧
短句来源
     The establishment of orthotopic liver transplantation in rat
     大鼠原位肝移植动物模型的建立
短句来源
     A modified animal model of orthotopic liver transplantation in rat
     改良“二袖套法”制备大鼠原位肝移植动物模型
短句来源
     Objective This study was conducted to build experimental model of orthotopic liver transplantation in rat(ROLT) with the character of acute rejection; and to study the effect of cytotoxic T lymphocyte antigen 4 immunoglobulin G(CTLA4-Ig) on prevention of rejection and the induction of immune tolerance of ROLT.
     目的建立大鼠原位肝移植(ROLT)急性排斥反应模型,探讨细胞毒淋巴细胞抗原4免疫球蛋白(CTLA4-Ig)抗排斥反应和诱导免疫耐受的作用。
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  rat liver transplantation
     Methods Rat liver transplantation was performed in 3 groups (NHBD 15,NHBD 30,NHBD 45) with 18 pairs in each, and the donor livers suffered 15,30 or 45 minutes from warm ischemia induced by cardiac arrest before being harvested respectively.
     方法 实验分为3组,在供肝获取前分别经历供体心脏停搏(NHBD)15、30或45min(NHBD15、NHBD30和NHBD45组),而后各行18对大鼠原位肝移植
短句来源
     Methods Rat liver transplantation was performed in 3 groups with 20 pairs in each (HB,NHBD 30 and NHBD 60),according to the non heart beating time 0,30 or 60 minutes before donor liver harvested.
     方法 实验以大鼠供肝获取前经历的心脏停搏时间 0、30和 6 0 min分为 3组 ,分别为 HB组、NHBD- 30组和 NHBD- 6 0组 ,而后行大鼠原位肝移植 ,每组各 2 0对。
短句来源
     Methods Orthotopic rat liver transplantation was performed in a Wistar-to-SD strain combination. All the transplantation models were divided into six groups: Group A(n=6),Wistar-to-Wistar or SD-to-SD;
     方法建立大鼠原位肝移植模型:①A组(n=6):同品系移植,供、受体同为W istar大鼠或SD大鼠;
短句来源
     Methods: Rat liver transplantation was performed in 3 groups of animals of 20 pairs in each (HB, NHBD-30 and NHBD-60) according to the duration of cardiac arrest of 0, 30 or 60 minutes before the donor liver was harvested.
     方法 :实验以大鼠供肝获取前供体所经历的心脏停搏时间 0、30和 6 0min将实验动物分为 3组 :HB组、NHBD 30组和NHBD 6 0组 ,而后行大鼠原位肝移植 ,每组各 2 0对。
短句来源
     Results Thirty-one cases of modified rat liver transplantation were done during this study. The successful rate of operation was 90.32 %. The 24 h- one week- and one month-survival rate posttransplant was 80.65 %, 74.19 % and 70.97 % respectively.
     结果共施行改良的大鼠原位肝移植31次,成功28次,手术成功率为90.32%,术后受者24h存活率为80.65%(25/31),术后1周存活率为74.19%(23/31),术后1个月存活率为70.97%(22/31)。
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  rat orthotopic liver transplantation
Total RNA differential display (DD) using random primers was performed for rat orthotopic liver transplantation (OLT) models.
      
Gold hamster to rat orthotopic liver transplantation is concordant and heterotra nsplantation and presents with acute rejection.
      
Mat on SEC injury caused by preservation and reperfusion using a rat orthotopic liver transplantation model.
      
  orthotopic liver transplantation in rats
The effect of cyclosporin on endothelin levels after orthotopic liver transplantation in rats
      
Hepatocellular metabolic change after orthotopic liver transplantation in rats
      
Biliary complications after orthotopic liver transplantation in rats
      
We recently experienced a high frequency of biliary complications after orthotopic liver transplantation in rats (22 of 25 cases (88%): biloma, 20 cases; biliary peritonitis, 2 cases).
      
  rat liver transplantation
Biological features of intrahepatic CD4+CD25+ T cells in the naturally tolerance of rat liver transplantation
      
The biological features of intrahepatic CD4+ CD25+ T regulatory cells in the naturally tolerance of rat liver transplantation were explored.
      
Warm Carolina rinse solution prevents graft failure from storage injury after orthotopic rat liver transplantation with arterial
      
Recently, a new solution, Carolina rinse solution, was shown to prevent reperfusion injury to endothelial cells in vitro almost completely and to improve graft survival after orthotopic rat liver transplantation (ORLT) without arterialization.
      
The beneficial effect of prostacyclin analogue (OP 2507) on rat liver transplantation subjected to an extended anhepatic phase
      
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  其他


To modified Miyata's and Kamada's technique of orthotopic liver transplantation (OLT) in rats and drew from Sun's certain experience and successfully made the model of OLT in rats. The operative success rate was 93.70% (119/127) on the average and 1-week survival rate was 89.76% (114/127). We observed immune respo nse in the OLT among the various species of rats available in China,which showed the longer survival time-in isografting of SD-SD. Wister-Wistar, Lou/c-Lou/c and TJR/1-TJR/1. The survival time of SD→Wistar...

To modified Miyata's and Kamada's technique of orthotopic liver transplantation (OLT) in rats and drew from Sun's certain experience and successfully made the model of OLT in rats. The operative success rate was 93.70% (119/127) on the average and 1-week survival rate was 89.76% (114/127). We observed immune respo nse in the OLT among the various species of rats available in China,which showed the longer survival time-in isografting of SD-SD. Wister-Wistar, Lou/c-Lou/c and TJR/1-TJR/1. The survival time of SD→Wistar or Wistar→SD was 22.65 days and 20.67 days respectively, which was not very suitable to be used as the models in the research on solving the problem of hepatic allogrft rejection, tut in the liver transplantation of Lou/c-SD, all recipients survived indefinitely. We used TJR/lrats as donors, in TJR/1-Lou/c and TJR/l-SD transplantation, the survival time was 15.50 days and 11 days respectivtly.The immune response in TJR/l-SD liver transplantation was the strongest. The combination of TJR/l-SD in OLT could provide ideal models for observing immune response of liver transplantation in rats.

总结127例大鼠原位肝移植模型的建立及体会。移植肝的血管重建采用肝上下腔静脉缝合加肝下下腔静脉及门静脉袖套法吻合。供受体手术共用时95~105分钟,移植肝冷缺血60分钟,无肝期17分钟。手术成功率93.70%(119/127),一周存活率89.76%(114/127)。在SD→SD、Wistar→Wistar、Lou/c→Lou/c、TJR/1→TJR/1、SD→Wistar、Wistar→SD、Lou/C→SD、TJR/1→Lou/c、TJR/1→SD九组不同品系的大鼠组合中筛选原位大鼠肝移植后呈现高免疫应答反应的鼠种,以便于观察肝移植排斥反应与免疫应答的关系。其中TJR/1→SD组移植肝平均存活11天,血清谷丙转氨酶及胆红素测定也显示肝功能受损明显,组织病理学检查呈急性排斥反应,为理想的大鼠肝移植高免疫应答模型。

A new operative model of orthotopic liver transplantation was established in the rat.The successful operation rate was 92.1%, one week survival rate 86.27%.The key points of operation were as follows: cold perfusion was performed on donor liver before operation, only portal vein was reconstructed in receiver, both double cuff and "8" typeface everting suture were used in the vein anastomosis, and biliary duct was reconstructed by end to end anastomosis of choledochus in single tube stand.The liver ultrastructure...

A new operative model of orthotopic liver transplantation was established in the rat.The successful operation rate was 92.1%, one week survival rate 86.27%.The key points of operation were as follows: cold perfusion was performed on donor liver before operation, only portal vein was reconstructed in receiver, both double cuff and "8" typeface everting suture were used in the vein anastomosis, and biliary duct was reconstructed by end to end anastomosis of choledochus in single tube stand.The liver ultrastructure showed that it was critical to control the immunologic rejection in 2 weeks following the operation, and that immunologic tolerance was produced 8 weeks after the transplantation in the experimental rats and then the liver graft survived.

用51只Wistar大鼠建立了新的大鼠原位肝移植手术模型,手术成功率为92.1%,周存活率为86.27%。手术要点是术前供肝冷灌注;受体肝只建立门静脉血供,静脉吻合分别采用“双袖套”式吻合和“8”字外翻缝合;胆管重建采用单管支架总胆管对端吻合法。移植术后肝细胞超微结构动态观察表明:术后2周是控制免疫排斥反应的关键时期,术后8周实验鼠产生免疫耐受性,移植肝脏成活。

In order to improve operation results and to decrease the complication rate of orthotopic liver transplatation in rats.65 orthotopic liver transplantations were performed by suturing suprahepatic vena.cava with 7-0 ptolene and using cuffs for infrahetic vena cava,portal vein and bile duct.The main complications in the experiment were bleeding, infection and bile duct obstruction.The cause of the Complications was analysed.The techniques of the operation that should be adopted for decreasing complica.tion were...

In order to improve operation results and to decrease the complication rate of orthotopic liver transplatation in rats.65 orthotopic liver transplantations were performed by suturing suprahepatic vena.cava with 7-0 ptolene and using cuffs for infrahetic vena cava,portal vein and bile duct.The main complications in the experiment were bleeding, infection and bile duct obstruction.The cause of the Complications was analysed.The techniques of the operation that should be adopted for decreasing complica.tion were presented in this article,which would be Useful to improve the results of the Operation.

为了改进手术操作,减少大鼠原位肝移植术的并发症,采取肝上下腔静脉缝合,肝下下腔静脉、门静脉及胆道用套管吻合法做了65次大鼠原位肝移植术。术后受体的主要并发症是出血、感染和胆道梗阻。该实验结果提示,术中注意防止血管损伤、提高手术技术是预防术后发生并发症的关键,另外,胆道套管的长度不宜超过5mm,否则易发生结石。

 
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