A review with 44 references is presented on the development of sol-gel-based biosensor. The main discussions are devoted to the process, advantages and properties of sol-gel immobilization method, sol-gel optical biosensor and amperometric biosensor, also the trend in this field is forecasted.
The catalyze mechanism of HRP, HRP-catalyzed synthesis of ortho-position or para-position replacing polyaniline, the immobilization methods of HRP and the immobilized HRP-catalyzed synthesis of polyaniline were also introduced.
The effects of two kinds of carriers and four different kinds of immobilized methods on the co-immobilization of tannase and β-glucosidase were investigated in this work. The results showed that the best co-immobilization could be obtained by selecting sodium alginate as the immobilized carrier and using the crosslinking-embedding-crosslinking method.
A method of immobilization of laccase Rhus vernicifera on urushiol resin (UR) with adsorbed metal ion is introduced. The activity of the UR-Al is better than that of UR-Fe immobilized laccase. The results show that the urushiol-salicylic acid grafted resin(USR)is better than UR for the support of immobilized laccase.
The high-efficiency atrazine-biodegrading bacterium from industrial sewerage, named as HB-5, was filtrated. The method of immobilization of bacterium HB-5 were studied. The differences of biodegradation data between bacterium HB-5 and immobilized HB-5 on soil were compared.
Enhancing enzymatic properties by the immobilization method
In this immobilization method, rabbit antiserum containing a specific polyclonal antibody against Vtg was directly immobilized on an antibody-binding Staphylococcal protein A matrix (SpA) without the need for prior purification.
Growth of Fusarium moniliforme on n-Alkanes: Comparison of an immobilization method with conventional processes
From an industrial point of view, the immobilization method described here has two advantages over other immobilization methods, i.e.
This immobilization method enabled direct air bubbling in the serum-free medium and a very high cell concentration (3×107 cells/ml gel) was obtained.
Immobilization methods that retain the biological activity of nucleic acids and provide an opportunity to use them as multipurpose analytical reagents are described.
The classification of immobilization methods is based on the chemical nature of the surface of the material used in the sensitive element of a sensor: noble metals; inogranic oxides and salts; carbon and polymeric materials.
Xylanase from Bacillus pumilus strain MK001 was immobilized on different matrices following varied immobilization methods.
Thermoanaerobacter cyclomaltodextrin glucanotransferase (CGTase) was immobilized using different supports and immobilization methods to study the effect on activity recovery.
Traditional bacteria immobilization methods such as calcium alginate entrapment have many application limitations in hydrogen fermentation, including limited duration time, bacteria leakage, cost, and so on.