Method: The K2CrO7 and H2O2 were used as mutagen; The isolated liver, spleen and kidney cells from mice were exposed to different concentrations of K2CrO7 and H2O2. The DNA damage detected by comet assay was used to judge the sensitivity of the cells.
It involves culturing the kidney cells in medium RPMI-1640 supplemented with 20% fetal calf serum at a temperature of 26℃, treating living cells with a final concentration of 10 μg/ml BrdU for 16-18h as well as with 2 μg/ml AMD (actinomycin D) along with 0.05 μg/ml colchi-cine for 1.5-2h before harvest.
METHODS: With modified calcium phosphate - DNA coprecipitation method the eukaryocytic expression plasmid expressing antisense fragment of hREV3,pBK - RSV - hREV3- and pMAMneo - amp hREV3 were transfected into human embryo kidney cell line of HEK - 293. After G418 selection, cell lines of 293 - B - hREV3- and 293 - M hREV3- were established.
Metheods The cellular membrane of the gastric carcinoma cell line BGC-823 and human normal kidney cell line 293 was made. Each of them was analyzed by radioactive ligand of LHRH-PE40 marked by 125I and their competitive binding with LHRH was detected.
Methods Different oral dose of NaF in aqueous solution was given to Wistar rats and renal tubular cells in vitro,the level of Ca2+ in serum, [Ca2+]i and Ca2+-ATPase of renal cells were assessed by biochemical methods.
We studied certain aspects of interaction between (3H)aldosterone and the cytoplasmic as well as nuclear receptors of renal cells in the rats during compensatory renal hypertrophy at the background of reflex renal dystrophy.
Angiotensin II (ANG II) has multiple effects on cardiovascular and renal cells, including vasoconstriction, cell growth, induction of proinflammatory cytokines, and profibrogenic actions.
ANG II-induced ROS play a pivotal role in several pathophysiologic situations of vascular and renal cells such as hypertension, endothelial dysfunction, nitrate tolerance, atherosclerosis, and cellular remodeling.
These findings suggest that pHGF may non-specifically promote the DNA synthesis of renal cells.
The results suggested that NaF induces the apoptosis in renal tubules via activation of the Bax expression and Bcl-2 suppression; OPN probably acts as protective role against apoptosis in fluoride-treated renal cells.