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肾细胞
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  kidney cells
Isolation and physicochemical properties of tankyrase of human embryonic kidney cells of line 293
      
We have isolated and purified endogenous cytosolic tankyrase from human embryonic kidney cells of line 293.
      
The cluster corresponding to normal, differentiated kidney cells included ERBB2 (HER2) for receptor protein-tyrosine kinase, several phosphatase genes (PTPRE, PTPRB, DUSP9), and EGF.
      
Two hybrid cell lines, KS-RL-3 (hybrid between TK- sheep kidney cells and rabbit lymphocytes) and CR-KS TK- (hybrid between rabbit β-cells and TK- sheep kidney cells), were assayed cytogenetically.
      
McCoy cells (mouse fibroblasts), HeLa 229 (derived from human cervical carcinoma cells) and BHK-21 cells (baby hamster kidney cells) are the cell types regularly used for the culture ofC.
      
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  kidney cell
Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293
      
In embryonic kidney cell line 293 the enzyme was excluded from the nuclei and distributed in fractions of soluble cytosolic proteins and low-density microsomes.
      
Recombinant NP was synthesized in Escherichia coliand in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase.
      
The genes that were induced and suppressed in human embryonic kidney cell line RH upon the infection with tick-borne encephalitis virus were studied by the method of subtractive hybridization.
      
Their cotransfection into the human embryonic kidney cell line HEK293T provided the production of a full-size recombinant human antibody.
      
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1.The tongue,urinary bladder,pericardial membrane,lung etc.,altogether 13 kinds of tissues were isolated from the common toad (Bufo bufo gargarizans) and cultivated,the tongue of frog (Bana plancyi) was used for comparison.Observations were made on cell growth and multiplication as well as on other behavior.2.Then the toad kidey and lung tissues as well as frog kidney tissue were selected for monolayer cell cultivation.It was demonstrated that the two commonly used culture media (GLPY and modified Eagle medium)...

1.The tongue,urinary bladder,pericardial membrane,lung etc.,altogether 13 kinds of tissues were isolated from the common toad (Bufo bufo gargarizans) and cultivated,the tongue of frog (Bana plancyi) was used for comparison.Observations were made on cell growth and multiplication as well as on other behavior.2.Then the toad kidey and lung tissues as well as frog kidney tissue were selected for monolayer cell cultivation.It was demonstrated that the two commonly used culture media (GLPY and modified Eagle medium) and the method for mono-layer cell culture could be well adapted for amphibian cell culture.3.To isolate cells from esplants for cultivation,the writer found that the use of 0.5% trypsin mixed with 0,01% EDTA and incubated at 25±1℃for a short period of digestion proved to be more satisfactory as compared with the methods used by earlier workers.This new method may be considered as a definite improvement.4.The cytological observations showed that although the epithelium tissues and cells from different sources appeared on the whole quite similar,yet the differences in cell growth and behavior as well as the capacity for multiplication were apparent.In maintaining the original characteristics of the defferent kinds of cells,it appeared that they manifested a certain degree of constancy and irreversibility.Thus it offers a great possibility to use such characteristics for the hybridization study of somatic cells.In this respect,it is to be noted that the ciliary movement of lung tissue from toad,after five months of culture in vitro was still maintained as before without showing least tendency of weakening.The cells of toad's urinary bladder,while migrating outward from the explant to form an outgrowth,showed very little activity of cellular multiplication.It thus appears that cell movement may be considered as a kind of manifestation for cellular differentiation,because it showed a tendency to inhibit cell division.It was further observed that cell division showed a certain degree of synchronization.This may mean that the rate and onset of cell division are determined by cytoplasmic and environmental factors.For example,chick embryonic extract can exert an obvious effect on cell multiplication.The discovery of synchronization as well as non-synchronization of the dinucleated cells and the multipolar divisions of some cells offers problems for further study,especially in view of the fact that a great deal of attention has been paid in recent years to the cytological studies of cell fusion etc.5.In the course of cell division,some abnormalities of metaphase figures,such as chromosome bridge formations were found.This is apparently due to the crossing-over within the heterozygous inverted regions of the chromosomes.While it may be a common occurence in meiosis but in mitosis it is extremely rare.The significance of the observations mentioned was discussed.

作者首先利用组织块培养法研究了中华大蟾蜍(Bufo bufo gargarizans)的舌、膀胱、心包膜、肺脏等十三种组织及金线蛙(Rana plancyi)舌组织的离体培养条件,并观察了在此条件下若干组织在体外生长与增殖的难易程度及表现。然后利用中华大蟾蜍的肾、肺细胞及金线蛙的肾细胞,确立了两栖类脏器组织细胞的单层培养方法。实验证明,本研究所确立的两种培养基(GLPY培养基与修改的Eagle培养基)及单层培养的方法,完全适用于两栖类细胞。在从组织块分离供培养的活细胞时,作者采用的0.5%胰蛋白酶与0.01%EDTA的混合消化液及在常温(25±1℃)下短期消化的方法,效果较好。这和前人在这方面的工作相比较,是一个改进。研究表明,虽然来源不同的上皮组织的细胞在离体培养条件下形态十分相似,但其生长表现与增殖能力却存在相当显著的差异。在各种不同的细胞尚能维持其原有特征这点上,似乎表明,体细胞分化有一定程度的不可逆性。这样,就为研究体细胞的遗传提供了极大的可能性。实验证明,中华大蟾蜍的肺细胞在离体培养五个月之后仍然维持正常的纤毛运动而不见衰退,这说明细胞在离体培养条件下还可以长期维持体细胞的特性。膀胱组织...

作者首先利用组织块培养法研究了中华大蟾蜍(Bufo bufo gargarizans)的舌、膀胱、心包膜、肺脏等十三种组织及金线蛙(Rana plancyi)舌组织的离体培养条件,并观察了在此条件下若干组织在体外生长与增殖的难易程度及表现。然后利用中华大蟾蜍的肾、肺细胞及金线蛙的肾细胞,确立了两栖类脏器组织细胞的单层培养方法。实验证明,本研究所确立的两种培养基(GLPY培养基与修改的Eagle培养基)及单层培养的方法,完全适用于两栖类细胞。在从组织块分离供培养的活细胞时,作者采用的0.5%胰蛋白酶与0.01%EDTA的混合消化液及在常温(25±1℃)下短期消化的方法,效果较好。这和前人在这方面的工作相比较,是一个改进。研究表明,虽然来源不同的上皮组织的细胞在离体培养条件下形态十分相似,但其生长表现与增殖能力却存在相当显著的差异。在各种不同的细胞尚能维持其原有特征这点上,似乎表明,体细胞分化有一定程度的不可逆性。这样,就为研究体细胞的遗传提供了极大的可能性。实验证明,中华大蟾蜍的肺细胞在离体培养五个月之后仍然维持正常的纤毛运动而不见衰退,这说明细胞在离体培养条件下还可以长期维持体细胞的特性。膀胱组织块培养的观察表明,细胞由组织块向外迁移与分裂是有矛盾的,迁移快则分裂少,看来细胞向外迁移也类

1.The pressent report consists of the karyotype analysis of cultured Amphibian somatic cells in vitro and the comparison of different cells from various tissues with regard to their karyotypes.2.The chromosomes of the cells from the tongue,kidney and lung tissues of the common toad (Bufo bufo gargarizans) were studied.They were found to possess the diploid chromosome number of 22 (2n = 22),i.e.12 large and 10 small chromosomes.The large chromosomes were matched into 6 homologous pairs and the small chromosomes...

1.The pressent report consists of the karyotype analysis of cultured Amphibian somatic cells in vitro and the comparison of different cells from various tissues with regard to their karyotypes.2.The chromosomes of the cells from the tongue,kidney and lung tissues of the common toad (Bufo bufo gargarizans) were studied.They were found to possess the diploid chromosome number of 22 (2n = 22),i.e.12 large and 10 small chromosomes.The large chromosomes were matched into 6 homologous pairs and the small chromosomes were matched into 5 homologous pairs.All of them were metacentric and submetacentric.According to the size,shape and position of the centromere,22 chromosomes were tentatively classified into 4 groups:Group 1-2,Group 3-6,Group 7-10 and Group 11.The chromosome pair 6 can be identified unequivocally by direct observation for the small satellites on the long arms.Sexual dimorphism of the chromosomes was not detected in somatic karyotype of male and female common toad.3.From the phenomenon of satellite association and the other behavior,it was concluded that the No.6 chromosome is the nueleolus organizer in the common toad cells.4.Similar studies were made on the karyotype of the cells from tongue and kidney of the frog (Rana plancyi).They were found to possess the diploid chromosome-number of 26 (2n = 26),i.e.10 large and 16 small chromosomes.The large chromosomes were matched into 5 homologus pairs and small chromosomes were matched into 8 homologus pairs.All of them were metacentric,submetaeentric and sub-telocentric.According to the size,shape and the position of the centromere,26 chromosomes were tentatively classified into 3 groups:Group 1,Group 2-5 and Group 6-13.The chromosome pair 9 can be identified unequivocally by direct observation for the secondary constriction on the long arms.Sexual dimorphism of the chromosomes was not detected in somatic karyotype of male and female frog.5.The karyotype of tongue cells and kidney cells were indistingishable from each other in quantitative characteristics,e.g.the relative length of each chromosome and the arm ratio (long arm/short arm).From the above investigation,it was concluded that the differentiation of the tissues cells was not reflected in the chromosomal morphology neither in the common toad nor in the frog.

本文报道离体培养的两栖类体细胞的染色体组型及不同组织细胞的染色体组型的比较分析结果。对于中华大蟾蜍(Bufo btfo gargarizans)离体培养舌细胞、肾细胞与肺细胞的染色体组型分析表明,其二倍体染色体数目为22个(2n=22),包括12个大型染色体,10个小型染色体。全部染色体可配成11对,均为中部和亚中部着丝点染色体。根据染色体的特征,可分为四组:即1—2组、3—6组、7—10组及11组。在第6染色体的长臂上发现随体。雌雄个体之间,并末发现与性别决定有关的异型染色体之存在。根据中华大蟾蜍第6染色体之间在随体和次缢痕部位的联合现象及其他有关现象,作者认为第6染色体是核仁组织者。对于金线蛙(Rana plancyi)离体培养舌细胞与肾细胞染色体组型的分析表明,其二倍体数目为26个(2n=26),包括10个大型染色体和16个小型染色体。全部染色体可配成13对,其着丝点为中部、亚中部和亚端部,根据染色体的特征,可分为三组:即1组、2—5组和6—13组。在第9染色体的长臂上发现次缢痕。雌雄个体之间,并未发现与性别决定有关的异型染色体之存在。离体培养的体细胞(舌细胞与肾细胞...

本文报道离体培养的两栖类体细胞的染色体组型及不同组织细胞的染色体组型的比较分析结果。对于中华大蟾蜍(Bufo btfo gargarizans)离体培养舌细胞、肾细胞与肺细胞的染色体组型分析表明,其二倍体染色体数目为22个(2n=22),包括12个大型染色体,10个小型染色体。全部染色体可配成11对,均为中部和亚中部着丝点染色体。根据染色体的特征,可分为四组:即1—2组、3—6组、7—10组及11组。在第6染色体的长臂上发现随体。雌雄个体之间,并末发现与性别决定有关的异型染色体之存在。根据中华大蟾蜍第6染色体之间在随体和次缢痕部位的联合现象及其他有关现象,作者认为第6染色体是核仁组织者。对于金线蛙(Rana plancyi)离体培养舌细胞与肾细胞染色体组型的分析表明,其二倍体数目为26个(2n=26),包括10个大型染色体和16个小型染色体。全部染色体可配成13对,其着丝点为中部、亚中部和亚端部,根据染色体的特征,可分为三组:即1组、2—5组和6—13组。在第9染色体的长臂上发现次缢痕。雌雄个体之间,并未发现与性别决定有关的异型染色体之存在。离体培养的体细胞(舌细胞与肾细胞)染色体的相对长度与臂比指数的测量统计值的比较分析表明,同一个体的不同组织的细胞之间,无论是中华大蟾蜍还是金线蛙,均无显著差异。因此,可以认为

A study of the karyotype of H.nwlitriz was carried out with short-period cultured kidney cells in vitro by the air-drying method with Giemsa staining.There is a diploid chromosome number of 48 in H.molitrix,the karyotype of which consists of 12 pairs of median,8 pairs of submedian and 4 pairs of subterminal centric chromosomes with a total number of chromosome arms amounting to 96.No terminal centric chromosome can be detected,neither mono-armed chromosome.The results obtained from the present study are quite...

A study of the karyotype of H.nwlitriz was carried out with short-period cultured kidney cells in vitro by the air-drying method with Giemsa staining.There is a diploid chromosome number of 48 in H.molitrix,the karyotype of which consists of 12 pairs of median,8 pairs of submedian and 4 pairs of subterminal centric chromosomes with a total number of chromosome arms amounting to 96.No terminal centric chromosome can be detected,neither mono-armed chromosome.The results obtained from the present study are quite different from those of early reports.Having observed and analysed the characteristics of chromosome morphology in the early,full-,and late-metaphase figures,the author holds that while analysing the karyotypes,the full-metaphase is superior to other metaphases.The full-metaphase should be taken as the criterion,not the late-metaphase.Nor the phase should be taken when the chromosomes are too contracted to see clearly.

研究鲢鱼染色体组型,用短期离体培养的肾细胞、空气干燥制片、Giemsa染色。鲢鱼染色体组型2n=48,其中中部着丝点染色体12对,亚中部着丝点染色体8对,亚端部着丝点染色体4对,染色体总臂数(AN)为96,没有端部着丝点染色体,没有单臂的染色体。与前人的工作有很大不同。观察了鲢鱼等几种鱼的培养细胞有丝分裂中期不同时相的染色体,并分析了中期各时相染色体的特点,认为在分析染色体组型时,须注意染色体的取相时期,其中以正中期为最好,因此,宜以正中期为准,而不宜以晚中期或染色体严重收缩时为准。

 
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