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   肾细胞 在 畜牧与动物医学 分类中 的翻译结果: 查询用时:0.817秒
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肾细胞
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  kidney cells
Isolation and physicochemical properties of tankyrase of human embryonic kidney cells of line 293
      
We have isolated and purified endogenous cytosolic tankyrase from human embryonic kidney cells of line 293.
      
The cluster corresponding to normal, differentiated kidney cells included ERBB2 (HER2) for receptor protein-tyrosine kinase, several phosphatase genes (PTPRE, PTPRB, DUSP9), and EGF.
      
Two hybrid cell lines, KS-RL-3 (hybrid between TK- sheep kidney cells and rabbit lymphocytes) and CR-KS TK- (hybrid between rabbit β-cells and TK- sheep kidney cells), were assayed cytogenetically.
      
McCoy cells (mouse fibroblasts), HeLa 229 (derived from human cervical carcinoma cells) and BHK-21 cells (baby hamster kidney cells) are the cell types regularly used for the culture ofC.
      
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  kidney cell
Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293
      
In embryonic kidney cell line 293 the enzyme was excluded from the nuclei and distributed in fractions of soluble cytosolic proteins and low-density microsomes.
      
Recombinant NP was synthesized in Escherichia coliand in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase.
      
The genes that were induced and suppressed in human embryonic kidney cell line RH upon the infection with tick-borne encephalitis virus were studied by the method of subtractive hybridization.
      
Their cotransfection into the human embryonic kidney cell line HEK293T provided the production of a full-size recombinant human antibody.
      
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Summary 1.The cell-cultured lapinized strain of swine fever virus was sucessfully propagated on PK primary cell monolayer in rolling bottles, LHHank's solution containing horse or calve serum was used as the medium and no antibiotics were added. The inoculum was a 0.3% of 1:10 spleen suspension of infected rabbit or a 2—4% cell cultured virus. After cultivation at 36℃ the media were havested and changed every 4 days. The titre of havested virus in every run were 2x10~4-5x10~4 in terms of rabbit infective titre....

Summary 1.The cell-cultured lapinized strain of swine fever virus was sucessfully propagated on PK primary cell monolayer in rolling bottles, LHHank's solution containing horse or calve serum was used as the medium and no antibiotics were added. The inoculum was a 0.3% of 1:10 spleen suspension of infected rabbit or a 2—4% cell cultured virus. After cultivation at 36℃ the media were havested and changed every 4 days. The titre of havested virus in every run were 2x10~4-5x10~4 in terms of rabbit infective titre. 2.Each dose of the lyophylized combined vaccine contains 0.015 ml cell-cuture lapinized strain of swine fever virus.70 batches of such vaccines have been tested for their protective ability against swine fever. All vaccinated pigs have been challenged with swine fever virus and protection was shown to be 100%, and they have been protected against swine erysipelas and swine pasteurellosis aswell. More than one thousand million pigs were vaccinated in the fields. and results of vaccination proved to be safe and satisfactory.

用转瓶培养猪肾细胞生产猪瘟细胞苗,本试验证明①用小黄牛血清或马血清或犊牛血清均可制出较高毒价的猪瘟细胞毒,②营养液中加青、链霉索各90—100μ/ml,接毒后维持液不用抗菌素,可以控制污染,並有利于毒的复制,③按营养液量接种猪瘟兔化毒脾毒0.3%(克)或2—4%细胞培养毒,均可制出毒价5×10~(-4)的细胞苗,但必须保持收液pH在6.8—7.0左右,④残存抗菌素在0.5μ/ml以下的细胞毒,配三联苗后对猪丹毒及猪肺疫菌影响不显著,⑤已连续制三联苗70余批,三种成份分别检验均达到规定标准,田间试使已接种猪1000万头以上,安全有效。

1. By cultivating the lapinized hog cholera vaccine virus(LHCV) on pig kidney cells in rolling bottles with increased volume of cultural media(3—4 times more than the amount used in the routine method), the titres in the first and second harvests of vaccine virus were quite similar to those obtained by the routine method. The titres in the second harvest passed most of the tests of 5×10~4 RID/1ml (Rabbit Infective Doses). Results of the tests met entirely the demand for the mass production of vaccine. 2. The...

1. By cultivating the lapinized hog cholera vaccine virus(LHCV) on pig kidney cells in rolling bottles with increased volume of cultural media(3—4 times more than the amount used in the routine method), the titres in the first and second harvests of vaccine virus were quite similar to those obtained by the routine method. The titres in the second harvest passed most of the tests of 5×10~4 RID/1ml (Rabbit Infective Doses). Results of the tests met entirely the demand for the mass production of vaccine. 2. The method of cultivating hog cholera vaccine virus in rolling bottles around an inclined axis and with deeper culture media(7—8 times more than in the routine method) produced titres in the first and second harvests or in a single harvest(7 days after inoculation) quite similar to those obtained by the routine method.They fitted well the requirements for vaccine production. 3. We also cultivated the LCHV in rolling bottles as cited above but with even much more culture media(8 times more) and additional aerati on. In this case the titres obtained in single harvests(7 days after inoculation)passed most of the 5×10~4RID/1 ml tests also. The data given above suggested that these three methods of cultivation might be applicable in actual vaccine production, with the only consideration that much greater amounts of culture media than used in the routine method of production had to be consumed in these cases. These modifications would much simplify the production of LCHV vaccine.

1、用增加培养液量(3—4倍)旋转法培养猪瘟弱毒细胞疫苗,第一收、第二收病毒收获液的毒价均可达到普通旋转培养法的滴度。二收的绝大多数批次毒价用兔检5万倍通过,符合制苗要求。 2、用深液(培养液量增加7—8倍)倾斜旋转法培养猪瘟弱毒细胞疫苗。第一收与第二收的毒液或培养7天一次全收的毒液,其大多数试验批次毒价均可达到制苗标准。 3、用深液(培养液量增加8倍)倾斜旋转通气法培养猪瘟弱毒细胞疫苗,接毒后培养7天一次全收毒液,大多数批次试验毒价用兔检5万倍通过。符合制苗要求。以上三种培养方法所得资料说明提高猪肾细胞产毒量是明显的,工艺是简便的,在生产上是可行的。对细胞提高繁殖病毒能力的问题进行了某些探索。

In view of the fact that vaccination of hogs against hog cholera with the lapinized hog cholera virus(LHCV)produced in hog kidney cell (HKC)cultures has the danger of inducing diseases in hogs by patho- gens originated from hog kidneys,we decided to conduct experiments on the cultivation of LHCV in goat testis(GT)and goat kidney(GK) cell cultures.The results showed that both GT and GK cell cultures could support the propagation of LHCV which was produced continuously wi- thout inducing CPE,the harvest of the...

In view of the fact that vaccination of hogs against hog cholera with the lapinized hog cholera virus(LHCV)produced in hog kidney cell (HKC)cultures has the danger of inducing diseases in hogs by patho- gens originated from hog kidneys,we decided to conduct experiments on the cultivation of LHCV in goat testis(GT)and goat kidney(GK) cell cultures.The results showed that both GT and GK cell cultures could support the propagation of LHCV which was produced continuously wi- thout inducing CPE,the harvest of the virus-containing fluid could be carried out more than once,and the primary cultures of GT and GK cells could be subcultured.The subcultured cells appeared to possess greater adaptibility to the environment than the primary culture because the cell sheet could maintain longer and still support the propagation of the virus. The virus titres of the harvested fluids from both the primary and the subcultured cultures were shown to be high enough to conform to the requirements of regular vaccine production. Experiments on the potency and safetiness of the subcultured virus were successfully performed,and it is suggested that it would he possible to utilize GTand GK cells instead of HKC in the production of hog cholera vaccine.

针对目前使用猪肾细胞生产猪瘟弱毒疫苗存在来自组织本身污染带来的潜在致病危险的情况,我们试验了用羊羔睾丸与肾细胞来生产猪瘟病毒。结果表明:猪瘟兔化弱毒适应羊羔睾丸与肾细胞,呈温和感染而不致细胞病变,可连续收获多次,产毒较高,毒价稳定。将原代培养的羊羔睾丸、肾细胞传一代后,细胞更加适应体外环境,维持时间较长,经再接毒后产毒效果仍然很好,均可达生产要求。由此看来,用羊羔睾丸、肾细胞代替猪肾细胞生产猪瘟病毒苗头很好,若进一步试验成功,可考虑用于生产。

 
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