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   肾细胞 在 水产和渔业 分类中 的翻译结果: 查询用时:0.041秒
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肾细胞
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  kidney cells
Isolation and physicochemical properties of tankyrase of human embryonic kidney cells of line 293
      
We have isolated and purified endogenous cytosolic tankyrase from human embryonic kidney cells of line 293.
      
The cluster corresponding to normal, differentiated kidney cells included ERBB2 (HER2) for receptor protein-tyrosine kinase, several phosphatase genes (PTPRE, PTPRB, DUSP9), and EGF.
      
Two hybrid cell lines, KS-RL-3 (hybrid between TK- sheep kidney cells and rabbit lymphocytes) and CR-KS TK- (hybrid between rabbit β-cells and TK- sheep kidney cells), were assayed cytogenetically.
      
McCoy cells (mouse fibroblasts), HeLa 229 (derived from human cervical carcinoma cells) and BHK-21 cells (baby hamster kidney cells) are the cell types regularly used for the culture ofC.
      
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  kidney cell
Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293
      
In embryonic kidney cell line 293 the enzyme was excluded from the nuclei and distributed in fractions of soluble cytosolic proteins and low-density microsomes.
      
Recombinant NP was synthesized in Escherichia coliand in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase.
      
The genes that were induced and suppressed in human embryonic kidney cell line RH upon the infection with tick-borne encephalitis virus were studied by the method of subtractive hybridization.
      
Their cotransfection into the human embryonic kidney cell line HEK293T provided the production of a full-size recombinant human antibody.
      
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  renal cells
We studied certain aspects of interaction between (3H)aldosterone and the cytoplasmic as well as nuclear receptors of renal cells in the rats during compensatory renal hypertrophy at the background of reflex renal dystrophy.
      
Angiotensin II (ANG II) has multiple effects on cardiovascular and renal cells, including vasoconstriction, cell growth, induction of proinflammatory cytokines, and profibrogenic actions.
      
ANG II-induced ROS play a pivotal role in several pathophysiologic situations of vascular and renal cells such as hypertension, endothelial dysfunction, nitrate tolerance, atherosclerosis, and cellular remodeling.
      
These findings suggest that pHGF may non-specifically promote the DNA synthesis of renal cells.
      
The results suggested that NaF induces the apoptosis in renal tubules via activation of the Bax expression and Bcl-2 suppression; OPN probably acts as protective role against apoptosis in fluoride-treated renal cells.
      
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The method of kideny cell-PHA (phytohemagglutinin)culture in vitro for thestudy of fish chromosomes was reported by Yamamoto, K. and Y. Ojima (1973). Inthis paper a new method for inducing fish chromosomes divisions is proposed. The PHAadministration was by pericardial cavity injection in vivo, instead of adding it into thecell culture medium in vitro. The injection does of PHA is 8-10μg/g (body weight),the injection position is better located at the base of pectoral fin, the time for responseneeds about 4-4.5...

The method of kideny cell-PHA (phytohemagglutinin)culture in vitro for thestudy of fish chromosomes was reported by Yamamoto, K. and Y. Ojima (1973). Inthis paper a new method for inducing fish chromosomes divisions is proposed. The PHAadministration was by pericardial cavity injection in vivo, instead of adding it into thecell culture medium in vitro. The injection does of PHA is 8-10μg/g (body weight),the injection position is better located at the base of pectoral fin, the time for responseneeds about 4-4.5 hours after injection. It needs only 7 hours to obtain a largenumbers of mitotic metaphase figures of kidney cell. About 30 species of teleost fisheswere examined in the experiments. e. g. Parabramis pekinensis, Megalobrama hof-fmanni, Barbodes denticulatus, Cirrhinus molitorella, Clarias fuscus, Monopterusalbus, Anguilla japonica, Ophicephalus argus, Tilapia mossambica etc. all givingsatisfactory results. The average percentage of mitotic division by PHA injectionmethod is 1.56 times than that of the kidney cell-PHA culture; 1.82 times of theblood-PHA culture and 4.51 times of the usual air-drying, reaching 9.86%. Thismethod has a double advantage being rapid and simple, and also adaptable to thefield work.

本文报导了PHA(Phytohemagglutinin 植物血球凝集素)体内注射制备鱼类肾细胞染色体的方法。在北京鳊、广东鲂、鲮鱼、倒刺鲃.胡子鲶、鳗鲡、乌鳢、罗非鱼等分属不同科、目的近30 种鱼类均获得较高的分裂指数。PHA 体内注射法的平均细胞有丝分裂指数为肾培养的1.56倍,血培养的1.82倍,常规空气干燥法的4.51倍,达到9.86%。研究结果表明,PHA 在体内同样具有刺激肾细胞有丝分裂的作用。PHA 注射剂量8—10μg/g(鱼体重),秋水仙素注射剂量4—6μg/g(鱼体重),胸鳍基部各一次注射,效应时间4—4.5小时,在7小时内便可获得大量中期分裂相。所建立的方法不需进行细胞体外培养,简便快捷,适用于实验室和野外现场制备鱼类染色体。

The susceptibility to grass carp reovirus (GCRV) of CAB-80, BCC, PHG, GCE GCTF-2 and GCK-84 (these cell lines were derived from crucian carp blastula embryos, herbivorous bream caudal fins, loach haploid gastrula embryos, grass carp gastrula embryos, grass carp caudal fins and grass carp kidney, respectively), as well as four clones isolated from GCK-84, were analyzed comparatively. Sensitivity of various degrees was confirmed in the infected cell lines (except PHG) among which GCK-84 was the most sensitive....

The susceptibility to grass carp reovirus (GCRV) of CAB-80, BCC, PHG, GCE GCTF-2 and GCK-84 (these cell lines were derived from crucian carp blastula embryos, herbivorous bream caudal fins, loach haploid gastrula embryos, grass carp gastrula embryos, grass carp caudal fins and grass carp kidney, respectively), as well as four clones isolated from GCK-84, were analyzed comparatively. Sensitivity of various degrees was confirmed in the infected cell lines (except PHG) among which GCK-84 was the most sensitive. It is demonstrated that GCRV, in vitro, has no strict species specificity. Typical symptom of muscular hemorrhage could be replicated when fingerlings of grass carp were infected with the virus which had been propagated in GCK-84. The virus produced by GCK-84, GCRF-2, CAB-80, BCC and PHG was titred in GCK-84 cells. The titers (TCID50/ml) were 8.24, 7.36, 2.90, 2.15 and 1.33 respectively. Sicells. difference was also observed in the four clones and GCK-84, with the titers ranging fro6.3 to 9.32. The results prescnted above predict the relative potential significance in the disease-resistant breeding of fish cell engineering.Serious damages in GCRV-infected GCK-84 cells could be seen with electron microscopy. The observed virus was in the shape of spherical particles having a size of 58 nm in diameter and a core with high electronic density approximately 38 nm in diameter on the average. The distribution of virions in cells could be divided into three patterns: existing individually in cytoplasm, congregating in large crystalline aggregates bound by membranceous structures, gathering together in an orderly or disorderly arrangement without a surrounding membrane.

比较研究了鲫鱼异倍体细胞系(CAB-80)、团头鲂尾鳍细胞系(BCC)、大鳞副泥鳅雌核发育单倍体胚胎细胞系(PHG)、草鱼胚胎细胞系(GCE)、草鱼尾鳍细胞系(GCRF-2)、草鱼肾细胞系(GCK-84)及其四个克隆对草鱼呼肠孤病毒(GCRV)的敏感性。证实了这些细胞(PHG除外)在不同程度上对GCRV敏感,其中以GCK-84的敏感性最强。这表明,在体外培养条件下,GCRV并无严格的种族特异性。用经GCK-84传代的病毒感染草鱼种,能复制出典型的出血病症状。用GCK-84检测了病毒在GCK-84、GCRF-2、CAB-80、BCC和PHG中的滴度(TCID_(50/ml)),其值分别为8.24,7.36,2.90,2.15和1.33。4个克隆与肾细胞系对病毒的敏感程度亦不尽相同,其滴度在6.3到9.32之间变化。上述结果对细胞工程抗病育种预示有较大的潜在意义。在电镜下可见GCRV对被感染的细胞造成了严重的破坏。病毒为平均直径58nm的球形颗粒,具有一个高度电子密度的核心,平均直径约为38nm。病毒在细胞中的分布方式有三种:即散布于细胞质中的、呈晶格状包于一膜状结构中的和整齐或不整齐地聚...

比较研究了鲫鱼异倍体细胞系(CAB-80)、团头鲂尾鳍细胞系(BCC)、大鳞副泥鳅雌核发育单倍体胚胎细胞系(PHG)、草鱼胚胎细胞系(GCE)、草鱼尾鳍细胞系(GCRF-2)、草鱼肾细胞系(GCK-84)及其四个克隆对草鱼呼肠孤病毒(GCRV)的敏感性。证实了这些细胞(PHG除外)在不同程度上对GCRV敏感,其中以GCK-84的敏感性最强。这表明,在体外培养条件下,GCRV并无严格的种族特异性。用经GCK-84传代的病毒感染草鱼种,能复制出典型的出血病症状。用GCK-84检测了病毒在GCK-84、GCRF-2、CAB-80、BCC和PHG中的滴度(TCID_(50/ml)),其值分别为8.24,7.36,2.90,2.15和1.33。4个克隆与肾细胞系对病毒的敏感程度亦不尽相同,其滴度在6.3到9.32之间变化。上述结果对细胞工程抗病育种预示有较大的潜在意义。在电镜下可见GCRV对被感染的细胞造成了严重的破坏。病毒为平均直径58nm的球形颗粒,具有一个高度电子密度的核心,平均直径约为38nm。病毒在细胞中的分布方式有三种:即散布于细胞质中的、呈晶格状包于一膜状结构中的和整齐或不整齐地聚集在一起但无膜包裹的。

The present paper deals with the karyotype of Sparus latus Houttuyn.

本文以市售黄鳍鲷(体重96—225g,体长150—250mm)的肾细胞为材料,注射入PHA,用空气干燥法制片,姬姆萨染色,对其染色体组型进行了分析。

 
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