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   肾细胞 在 基础医学 分类中 的翻译结果: 查询用时:0.829秒
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肾细胞
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  kidney cell
Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293
      
In embryonic kidney cell line 293 the enzyme was excluded from the nuclei and distributed in fractions of soluble cytosolic proteins and low-density microsomes.
      
Recombinant NP was synthesized in Escherichia coliand in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase.
      
The genes that were induced and suppressed in human embryonic kidney cell line RH upon the infection with tick-borne encephalitis virus were studied by the method of subtractive hybridization.
      
Their cotransfection into the human embryonic kidney cell line HEK293T provided the production of a full-size recombinant human antibody.
      
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  renal cells
We studied certain aspects of interaction between (3H)aldosterone and the cytoplasmic as well as nuclear receptors of renal cells in the rats during compensatory renal hypertrophy at the background of reflex renal dystrophy.
      
Angiotensin II (ANG II) has multiple effects on cardiovascular and renal cells, including vasoconstriction, cell growth, induction of proinflammatory cytokines, and profibrogenic actions.
      
ANG II-induced ROS play a pivotal role in several pathophysiologic situations of vascular and renal cells such as hypertension, endothelial dysfunction, nitrate tolerance, atherosclerosis, and cellular remodeling.
      
These findings suggest that pHGF may non-specifically promote the DNA synthesis of renal cells.
      
The results suggested that NaF induces the apoptosis in renal tubules via activation of the Bax expression and Bcl-2 suppression; OPN probably acts as protective role against apoptosis in fluoride-treated renal cells.
      
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Though the study on cultivation and molecular bialogy of HSV has beenfar advanced, the pathogenicity and the interactions between HSV and diffe-rent cells, especially the most sensitive cells still require to elucidate indepthso as to establish a complete speetrum of patholog ical changes in different cells. The patterens of multiplication and specific cytopathic effects on sensitivetissue culture cells have been studied by using primary rabbit kidney tissueculture cells (PRK). The results are as follows:1....

Though the study on cultivation and molecular bialogy of HSV has beenfar advanced, the pathogenicity and the interactions between HSV and diffe-rent cells, especially the most sensitive cells still require to elucidate indepthso as to establish a complete speetrum of patholog ical changes in different cells. The patterens of multiplication and specific cytopathic effects on sensitivetissue culture cells have been studied by using primary rabbit kidney tissueculture cells (PRK). The results are as follows:1. The experiments proved that the eclipse period was 1:3 hrs by direct tit-ration method with the infecting dose of 100 TCID 50/ml on PRK cells. Thetitec of the virus went up to 10~2. ~(17)TCID 50/0. 1ml 24 hrs after infections. Itsoared repidly with 24-48 hrs and kept rising for 48-72 hrs with tites reaching10~(6.5) TCID 50/0. 1ml. When the dynamics of mhltiplication was indicated byCPE, the same results were obtained.2. The pathologic festures were described systimatically. The change ofintranuclear structure disorder of chromatin and its reorganization, the forma-tion of intracytoplasmic and intranuclear acidophilic inclusion bodies and theformation of giant synthetial cells as well were the main features of CPE. There is a discussion about the results in this paper.

用敏感的兔肾细胞培养HSV-1,对HSV的生长规律及致CPE的特点进行了研究。主要的细胞病理学变化是细胞核内部结构改变,核染质紊乱及重新组合,形成嗜酸性包涵体,细胞融合成巨大合胞体。

A comparative study of the early appearance and localization of HSV antigen

用免疫酶标与荧光标间接法比较研究了 HSV 抗原在兔肾细胞上出现的规律及定位。结果表明用100TCID_(50)HSV-1感染兔肾细胞时,感染后9小时可在胞核见有病毒抗原,12小时在胞浆中见有,24~48小时在胞核、胞浆内发现有大量的 HSV 抗原。

Essential -media tested were Earle, Earle +0.5% lactalbumin hydrolysate (Oxoid) and RPMI-1640. Baby jird testis and kidney tissue cell lines were used for culture. The most: favourable condition for the maintenance of the larvae in vitro was in a range of pH 6.4-7.5 and at temperatures of 36.5-37.5℃. The baby jird testis cell in growth medium RPMI-1640 was the best cell line tested with increases of 32.6% of the length and 36.8% of the width of the larvae taking place in 38 days cultivation. We observed successful...

Essential -media tested were Earle, Earle +0.5% lactalbumin hydrolysate (Oxoid) and RPMI-1640. Baby jird testis and kidney tissue cell lines were used for culture. The most: favourable condition for the maintenance of the larvae in vitro was in a range of pH 6.4-7.5 and at temperatures of 36.5-37.5℃. The baby jird testis cell in growth medium RPMI-1640 was the best cell line tested with increases of 32.6% of the length and 36.8% of the width of the larvae taking place in 38 days cultivation. We observed successful moulting in both MT and MK cell lines. Brugia malayi began to moult on the tenth day. The survival of the larvae maintained on MK cell and MT cell were prolonged to 23 and 38 days respectively.

马来丝虫感染期幼虫(L_3)在沙鼠睾丸细胞系培养液中发育良好,一般在10~14天开始蜕皮进入第四期幼虫(L_4),成双地扭集在睾丸细胞系中最长可活38天,幼虫长度增加32.6%,宽度增加36.8%,可初步分辨雌雄;在鼠肾细胞系培养液中L_3于10~20天开始蜕皮,少数进入L_4,最长可活23天,幼虫长度增加25.9%,宽度增加34.5%,L_3在单纯Earle、Earle加水解乳蛋白及RPMI-1640中,仅能活2~4天。在培养过程中应防止细菌污染,合适的温度和pH亦系培养成功的重要因素。

 
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