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   肾细胞 在 感染性疾病及传染病 分类中 的翻译结果: 查询用时:0.853秒
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肾细胞
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  kidney cells
Isolation and physicochemical properties of tankyrase of human embryonic kidney cells of line 293
      
We have isolated and purified endogenous cytosolic tankyrase from human embryonic kidney cells of line 293.
      
The cluster corresponding to normal, differentiated kidney cells included ERBB2 (HER2) for receptor protein-tyrosine kinase, several phosphatase genes (PTPRE, PTPRB, DUSP9), and EGF.
      
Two hybrid cell lines, KS-RL-3 (hybrid between TK- sheep kidney cells and rabbit lymphocytes) and CR-KS TK- (hybrid between rabbit β-cells and TK- sheep kidney cells), were assayed cytogenetically.
      
McCoy cells (mouse fibroblasts), HeLa 229 (derived from human cervical carcinoma cells) and BHK-21 cells (baby hamster kidney cells) are the cell types regularly used for the culture ofC.
      
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  kidney cell
Soluble Tankyrase Located in Cytosol of Human Embryonic Kidney Cell Line 293
      
In embryonic kidney cell line 293 the enzyme was excluded from the nuclei and distributed in fractions of soluble cytosolic proteins and low-density microsomes.
      
Recombinant NP was synthesized in Escherichia coliand in human kidney cell line 293 cotransfected with recombinant vaccinia virus vTF7-3 expressing T7 RNA polymerase.
      
The genes that were induced and suppressed in human embryonic kidney cell line RH upon the infection with tick-borne encephalitis virus were studied by the method of subtractive hybridization.
      
Their cotransfection into the human embryonic kidney cell line HEK293T provided the production of a full-size recombinant human antibody.
      
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This paper reports the detection results of HBV DNA sequences state in liver and kidney cell genomes of fetuses aborted from HBsAg positive mothers by Southern blot hybridization technique and cloned HBV DNA as probe labelled with 32P. In 40 fetuses,specific hybridization bands corresponding to high molecular weight DNA over 3. 2 kb of HBV DNA were observed in cellular DNAs extracted from 2 cases of fetal liver and I case of fetal kidney tissues after digestion by EcoRI and hybridization with 32P HBV DNA probe....

This paper reports the detection results of HBV DNA sequences state in liver and kidney cell genomes of fetuses aborted from HBsAg positive mothers by Southern blot hybridization technique and cloned HBV DNA as probe labelled with 32P. In 40 fetuses,specific hybridization bands corresponding to high molecular weight DNA over 3. 2 kb of HBV DNA were observed in cellular DNAs extracted from 2 cases of fetal liver and I case of fetal kidney tissues after digestion by EcoRI and hybridization with 32P HBV DNA probe. The results showed that HBV DNA sequences could integrate into hepatocyte and kidney cell genomes. The integration of HBV DNA in cell genomes might occur randomly since the integrating bands in fetal celluar DNA did not appear at same position. HBV DNA sequence was not found in the remaining tissue samples. None of free HBV DNA hybridization band could be detected in the cellular DNAs extracted from all fetal organs studied.

采用核酸分子杂交Southern印迹法,以32P标记的HBVDNA为探针,检测HBsAg阳性母亲引产的40例胎儿的肝、肾组织。结果有2例胎肝和1例胎肾细胞DNA出现大于3.2kb的杂交带,表明HBVDNA已处于整合状态。胎肾细胞基因组中查出HBVDNA整合为首次报道。

The rables vaccination schedule' with Beijing primary hamster kidney cells rabies vaccine was studied in 157 cases of rabies postexposure patients by dog-bite in Xuantan district Lu County, Sichuan Province from 1990~1991 in our hmpital. The patients wer divided into four group by different vaccination schedules. The aix serum samPbo taken on day 0 (before immunization), 15, 30, 60, 90, 180 after immunization were measured in one time for anti-rabies antibodies. The results showed that only after group Ⅱand...

The rables vaccination schedule' with Beijing primary hamster kidney cells rabies vaccine was studied in 157 cases of rabies postexposure patients by dog-bite in Xuantan district Lu County, Sichuan Province from 1990~1991 in our hmpital. The patients wer divided into four group by different vaccination schedules. The aix serum samPbo taken on day 0 (before immunization), 15, 30, 60, 90, 180 after immunization were measured in one time for anti-rabies antibodies. The results showed that only after group Ⅱand Ⅳ had developed the satisfied titre of proective antifories,which were higher than group Ⅰ and Ⅱ on day 30, and maintained longer (180 day),and tkere were significant differences between various group(P <0. 005). The immunication schedule of group Ⅱ could get the proective effects and save one dose of vaccine and two times of injection. So we recommend the vactination schedule of group Ⅲ.

报道我院1990~1991年对四川省泸县玄滩区157例大伤患者用国产地鼠肾细胞狂犬病疫苗进行免疫程序研究。在157例犬伤患者按不同免疫程序随机抽样分为4组,在免疫前(即O日)和免疫后15,30,60,90,180日取血分离血清,6次血清进行一次检测。其结果以Ⅲ组和Ⅳ组产生的保护性抗体效价较满意,在30日出现较高,维持时间最长(180日),均有高度显著性差异(P<0.005)。Ⅲ组免疫程序可节约一剂疫苗,减少两次注射时间,又达到保护作用,为此,我们推荐Ⅲ给免疫程序.

To elucidate the protective mechanism of cellular immunityof inactivated vaccine againstepidemic hemorrhagic fever (EHF), we assayed the lymphocyte blastogenesis, responses of interleukin-2(IL-2)and immune interferon (IFNr ) in mice immunized with inactivated vaccine against EHF cultivatedin golden hamster kidney cells. It was found that the proliferating indices of spleen cells stimulated withEHF virus antigen and Con A in immunized mice were significantly higher than those in normal mice. Nodifference in levels...

To elucidate the protective mechanism of cellular immunityof inactivated vaccine againstepidemic hemorrhagic fever (EHF), we assayed the lymphocyte blastogenesis, responses of interleukin-2(IL-2)and immune interferon (IFNr ) in mice immunized with inactivated vaccine against EHF cultivatedin golden hamster kidney cells. It was found that the proliferating indices of spleen cells stimulated withEHF virus antigen and Con A in immunized mice were significantly higher than those in normal mice. Nodifference in levels of IL-2 and IFNr produced by spleen cells cocultured with Con A was observed in bothimmunized group and controls, however, the level of IL-2 stimulated with EHF virus antigen in the im-munized mice were obviously higher than that in the controls. 9 of 15 immunized mice produced an IFNrresponse to stimulation of EHF virus antigen. The serum conversion rate of IFA antibodies was 93.3%in the immunized. The above results are valuable in evaluating the immune effects of the inactivated vac-cine against EHF.

为阐明流行性出血热(EHF)灭活疫苗的细胞免疫保护性机制,对地鼠肾细胞培养灭活疫苗免疫小鼠的脾细胞增殖,产生白细胞介素-2(IL-2)和干扰素(IFN)的能力进行了研究。结果表明,疫苗免疫小鼠脾细胞对ConA及EHF病毒抗原的刺激指数均显著高于正常小鼠。两组小鼠脾细胞在ConA刺激下产生IL-2和IFN,的水平无显著差异,但免疫小鼠受EHF病毒抗原刺激后产生的IL-2水平明显高于正常对照组;15只免疫小鼠有9只对病毒抗原的刺激产生IFN反应;免疫小鼠血清特异性抗体阳转率为93.3%。上述结果对于评价EHF灭活疫苗的免疫效果具有重要价值。

 
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