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培养结果
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  culture results
     The culture results showed that C—17 medium supplemented with CH 50mg/l+Cm 200ml/l+Inositol 100mg/l+Sucrose 90g/l is more suitable to the hybrid immature embyos growing and seedling.
     培养结果表明,C—17基本培养基添加CH50mg/L、CM200mL/L、肌醇100mg/L及蔗糖90g/L较利于杂种幼胚生长成苗。
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     The correspondence rate of the culture results between the sputum and the hands was 60.7%.
     医护人员的手采集标本培养结果与痰培养结果符合率较高为 60 .7%。
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     The culture results indicated that the rooting rate and the rate of grow into useful seedlings all reached 100%,and that the mean number of roots reached 5.83~6.27,and the effective propagation coefficient reached (5.50)~6.23 at the 30 d.
     培养结果表明,Cy 的生根率和成苗率均达100%,平均根数达5.83~6.27条,有效繁殖系数达5.50~6.23.
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     Analysis of the anaerobic bacteria culture results of 220 cases abdominal infections specimens.
     220例腹部感染标本厌氧菌培养结果分析
     RESULTS From 119 cases of patients were earned the routine bacteria culture results,the tested rate was 96.75%,the positive specimens were 379 and the postitive rate was 83.85%.
     结果其中119例患者标本送细菌培养,送检率为96.75%; 培养结果阳性标本379份,阳性率为83.85%;
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  results of culture
     The results of culture were 9 cases of bacterias and 39 cases of fungis.
     培养结果检出细菌9例,真菌39例。
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     Ficoll density gradient was used to collect and depurate protoplasts. The protoplasts from the 3 rd, and 4 th layer got better results of culture than the 1st, 2nd and 5th layer.
     用Ficoll密度梯度离心法收集原生质体进行培养,结果表明第三、四层界面的原生质体有较好的培养效果。
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     Methods The data (such as basic diseases, predisposing factor, results of culture and treatment) of 34 cases of confirmed fungal CRS were analyzed retrospectively.
     方法回顾性分析34例真菌性CRS患者的基础疾病、易患因素、导管真菌培养结果、治疗措施。
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  “培养结果”译为未确定词的双语例句
     The results of inducing callus and cell suspending cultivation showed that the medium for inducing callus is MS + 0.2 mg·L~(-1) 6-BA + 0.1 mg·L~(-1)NAA + 1.0 mg·L~(-1) 2,4-D + 3.0 g·L~(-1) PVP;
     对人心果愈伤组织的诱导及细胞悬浮培养结果表明,人心果愈伤组织诱导培养基为MS+6 BA0.2mg/L+NAA0.1mg/L+2,4 D1.0mg/L+PVP3.0g/L;
短句来源
     Depended on the result of the test on the QL+TDZ (0.5、1.0、2.0、4.0mg/L)+ NAA (0.25、0.75、1.25mg/L), TDZ was important than NAA for regeneration and callus inducing.
     在QL+TDZ(0.5、1.0、2.0、4.0mg/L)+NAA (0.25、0.75、1.25mg/L)培养基上的培养结果表明,TDZ 浓度对山杏叶片的愈伤组织诱导率和再生频率的作用大于NAA。
短句来源
     The different combinations of plant growth regulators were added into MS medium to investigate their effects on tissue culture of leaf as explants and 100% callus was inducted on MS media with 2.0 mg/L 2,4-D and 0.5 mg/L 6-BA.
     以叶片为外植体,在含不同生长调节物质的MS培养基中培养,结果表明,在MS培养基上附加2,4-D 2.0 mg/L和6-BA 0.5 mg/L对愈伤组织的诱导率可达100%;
短句来源
     3. Embryos of were cultured in the medium of l/2MS+0.1% IAA+0.1% 6-BAP+2% sucrose+7.5% agar(PH=5.8). The results showed that there was the best effect when embryos of 470~510μm were cultured and the frequency of seedling production was 59.1%~61.9%.
     3.选择不同大小幼胚在1/2 MS+0.1% IAA+0.1% 6-BAP+2% 蔗糖+7.5% 琼脂(PH=5.8)的培养基上培养结果表明,470~510 μm幼胚的培养效果最好,幼苗产生率为59.1%~61.9%。
短句来源
     The stem tip and stem segment of the Cymbidiuml owianum were good explants. The culture medium was MS+6-BA 2.0 mg/L+NAA 0.5 mg/L for callus induction.
     以碧玉兰的茎尖、茎段作为外植体进行组织培养,结果表明:适宜的起始培养基是MS+6-BA 2.0 mg/L+NAA 0.5 mg/L;
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  culture results
coli culture results in oscillations of the topological state of DNA.
      
However, incubation of isolated macrophages in the presence of dextran sulfate and such medium transfer into hepatocyte culture results in even more pronounced increase in TAT activity.
      
The addition of a carbon and energy source to this culture results in a second stress reaction.
      
The culture results showed that Spirulina platensis grows successfully in diluted human urine, and yields maximal biomass at urine dilution ratios of 140~240.
      
Furthermore the culture results were related to the radiographic extension of pulmonary infiltrates (92% positive cultures in multilobarvs 54% in lobar or segmental infiltrates, p>amp;lt;0.001).
      
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  results of culture
The diagnosis was based on results of culture and the response of the patient to long-term penicillin-derivate therapy after surgical drainage of the suprarenal abscess formation.
      
Results of culture on the enrichment medium were compared with those obtained on routine agar media and on a yeast differential agar which facilitates detection of mixed yeast species by their colony colours.
      
Such results were compared with the results of culture and indicated that the assay is as sensitive as bacteriological methods, though faster.
      
Results of culture of thirty soil samples obtained from the Judean Desert on the western side of the Dead Sea are reported.
      
The results of culture-tests performed on various textiles, the colony-formation of the more important dermatophytes on various textiles are discussed.
      
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Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different...

Cotton seeds collected from plants infected by Verticillium ablo-atrum were used for the purpose of studying seed transmission of the pathogen. Although verticilliate conidiophores had been observed occasionally under low-power microscope on the surface of the seeds plated on PDA in 1955, yet isolations of the organism failed due to the interference of several contaminants. Seeds were delinted in order to diminish the possibility of interference from lint-born contaminants. The materials were plated on different culture media such as PDA, acidified PDA, cotton meal agar and rice agar. All these plates failed to show verticillium colonies. Among the fungi obtained in these plates, Fusarium, Alternaria and Colletotrichum were the most frequent. Since both Verticillium albo-atrum and other fungi were harbored within the same seed, the difference of growth rates might be considered as an important factor involved in the difficulty of the isolations: Cultures of V. albo-atrum and Fusarium sp. were transferred simutaneously to PDA plates to test their growth rates. The diameter of their colonies were measured after incubation for 12 days under 22±1℃. Those of V. albo-atrum measured 2.8 cm., while the average of the latter was 9,3 cm. Interference of the fast-growers might render a slow-grower such as V. albo-atrum to be masked in the observations and to be depressed in the isolations. A method was developed to avoid the interference. The fundamental procedures of the method are outlined as follows. 1. Plating and observation. (1) Cotton seeds being delinted with commercial surphuric acid in order to avoid the interference from lint-born contaminants. (2) Delinted seeds being washed in a flask covered with wire gauze for 24 hours under running tap water in order to get rid of bacteria. Absorption of sufficient water during washing activates the hibernating mycelium. (3) Seeds being plated on 1.7% water agar instead of nutrient agar in order to diminish the growth of the fast-growers such as Fusaria and Alternaria. The plates being incubated under 22±1℃. for 15 days. (4) The plates being uncovered and observed directly under low-power microscope in order to observe the fungi undisturbed. 2. Isolation and preservation of the Verticillium cultures. (1) Verticilliate conidiophores being located under low-power microscope. (2) The tip of a flamed needle moistened with sterile agar being introduced within the microscopic field in order to fish the spores under focus. (3) Spore suspension being made by washing the needle in a 5 cc sterile water blank. (4) Dilution plates being made by placing 1 cc of the spore suspension in a petri dish and diluting with 10 cc of cotton stem agar. (5) The plates being observed under low-power microscope after incubation for 5—7 days at 22±1℃. (6) Verticillium colonies being locatad and a bit of the margin of a colony being transferred to PDA slant. (7) The slant cultures being flooded with sterile liquid paraffin after incubation for 7—15 days at 22±1℃. The cultures preserved with liquid paraffin were able to keep alived for 2 years. Seeds collected from the infected plants in 1955, 1956 and 1957 were studied. The percentages of seeds carrying Verticillium observed in these years were 39.8, 5.92 and 9.5% respectively. One of the representative single spore colonies obtained was identified as V. albo—atrum. Inoculation and reisolation tests showed positive results. Inspection of cotton seed samples collected from eight co-op farms in Shensi Province in 1958 showing that the percentages of seeds infected by verticillium ranged 3 to 23% with an average of 12.3%.

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号...

(1) 在陝西关中所采的黄萎病病株棉籽內部,常带有一些真菌和細菌,虽經用浓硫酸脫絨,这些菌类仍能从棉籽本身的組織內向培养基伸展惺庇玫捅剁R直接检查虽亦能見到輪枝菌从棉籽內露出,但由于其他菌类的生长速度較快,以致輪枝菌常被干扰,无从在洋菜上发展,故用一般方法很难分离和检查到棉籽所带的輪枝菌。(2) 用不含养分的水洋菜作为培养基时,杂菌的生长減弱,轮枝菌才能在基物上发展为菌落。又用流水冲洗法代替表面消毒,使棉籽吸收水分,同时也促使潛伏菌絲继續发展,并可冲去細菌。經試驗用上述两个步驟可以使棉籽所带的輪枝菌在水洋菜上形成菌落。結合低倍鏡直接检查法,可以較为方便地检查种籽带菌率。(3) 經初步拟定了一套检查方法和单孢子菌种分离法。惟应用时尚觉不够簡便,尚有待于进一步提高,特提出以供交流經驗。(4) 在1955—1957年的多次检查中,查得黄萎病病株棉籽带輪枝菌率为39.8%、5.9%及23.7%。在1958年初对大田种子进行检查,查得华阴等四县8个样品中带菌率为3%至23%,平均为12.3%。(5) 根据棉籽解剖培养結果,轮枝菌不仅存在于棉籽外部的短絨內,并且也存在于籽壳及籽仁上。(6) 从棉籽上分离到的轮枝菌特8号經初步鉴定系Verticillium albo-atrum Reinkeet Berth。(7) 特8号菌种經过接种試驗,确定其具有一定的致病力,并从再分离中获得該菌。(8) 棉籽是否能够传播黄萎病,似系一个检查方法問題。还希各方面进行测定,通过多次的检查和接种試驗始能充分确定。(9) 在分离的菌种中,除特8号菌种具有較深的菌落色泽外,还有一些菌种的顏色較淡,未須鑑定。棉籽所带的轮枝菌类型不一,还有进一步研究的必要。此外,棉籽內的輪枝菌和其他一些菌类究竟如何进入籽內,也有研究的价值。

The effects of the composition and pH of different culture media on the amino acid decarboxylase of five strains of E. coli were studied, and a solid medium consisting of beef broth, peptone(1%) and agar(2%) at pH 7.5 was selected to cultivate the bacteria for preparing L-glutamic acid decarboxylase. An acetone powder was prepared from E. coli grown in the solid medium at 37℃ for 18-20 hours. It decarboxylated L-glutamic acid with a high specificity.

根据五株大腸杆菌在不同成分与pH的培养基內培养的結果,选择了pH7.5的牛肉湯加蛋白腖与琼脂的固体培养作为細菌培养条件。从18—20小时在37度培养的大腸杆菌制成了高产量的专一性谷氨酸脫羧酶丙酮粉,其最适pH为5.1,米氏常数为4.3×10~(-3)M。拟定了一种血清及动物組織中轉氨基酶的測定法。此方法是利用大腸杆菌谷氨酸脫羧酶丙酮粉测定轉氨基作用所生成的谷氨酸量。应用此方法測得我国正常成人血清的谷門轉氨基酶活性为0.70±0.22单位与谷丙轉氨基酶活性为0.58±0.25单位(每单位等于在37度轉氨基作用生成1微克分子L-谷氨酸/1毫升血清/1小时)。应用此方法也試測了大白鼠六种組織中五种氨基酸对α-酮戊二酸的轉氨基酶活性。

o-Bis (β-chloroethyl)-aminomethyl-phenylalanine dihydrochloride (I, AT-581) was re- ported to possess significant antitumour activities. Furthermore, in view of the fact that compounds carrying a nitro group in the benzene ring show invariably higher antitumour activity as compared with the corresponding unsubstituted ones and that the electro- negative groups such as nitro can deactivate the chlorine atoms of the mustard grouping, thus decrease its toxicity, the authors synthesized 2-bis-(β-chloroethyl)-aminomethyl-5-...

o-Bis (β-chloroethyl)-aminomethyl-phenylalanine dihydrochloride (I, AT-581) was re- ported to possess significant antitumour activities. Furthermore, in view of the fact that compounds carrying a nitro group in the benzene ring show invariably higher antitumour activity as compared with the corresponding unsubstituted ones and that the electro- negative groups such as nitro can deactivate the chlorine atoms of the mustard grouping, thus decrease its toxicity, the authors synthesized 2-bis-(β-chloroethyl)-aminomethyl-5- nitro-phenylalanine(IV) in which a nitro group was introduced into the benzene ring of AT-581. Besides, 2-bis(β-chloroethyl)-aminomethyl-4-nitrotoluene(IV) was also synthe- sized. 2-Methyl-5-nitrobenzyl chloride (VII) was condensed with diethyl formamido-malo- nate to give diethyl 2-methyl-5-nitrobenzyl formamido-malonate (VIII), and the latter was readily converted to diethyl 2-bromomethyl-5-nitrobenzyl formamido-malonate(X) by N-bromosuccinimide in the presence of a small amount of dibenzoyl peroxide in anhydrous carbon tetrachloride. Treatment of X with diethanolamine afforded diethyl 2-bis(β- hydroxyethyl)-aminomethyl-5-nitrobenzyl formamido-malonate(XI), which was then chlorinated with thionyl chloride in dichloromethane to give the chloride (XII),and the desired product (IV) was obtained by the hydrolysis of XII with concentrated hydro- chloric acid. When compound VII was first treated with diethanolamine and then with thionyl chloride, compound VI was obtained.

在邻双(β-氯乙基)氨甲基苯丙氨酸(I,AT-581)苯环的5-位上引进一个硝基,合成了化合物IV.作者希望能通过硝基的拉电子作用降低氮芥基上氯原子的活泼性,从而减低AT-581的毒性并增加其抗癌活性.在同样的设想下,作者又在邻甲基苄基氮芥(V)苯环的4-位上引入一个硝基,合成了化合物VI.组织培养结果表明化合物IV和VI对HeLa细胞具有抑制作用.

 
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