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coli culture results in oscillations of the topological state of DNA.
      
However, incubation of isolated macrophages in the presence of dextran sulfate and such medium transfer into hepatocyte culture results in even more pronounced increase in TAT activity.
      
The addition of a carbon and energy source to this culture results in a second stress reaction.
      
The culture results showed that Spirulina platensis grows successfully in diluted human urine, and yields maximal biomass at urine dilution ratios of 140~240.
      
Furthermore the culture results were related to the radiographic extension of pulmonary infiltrates (92% positive cultures in multilobarvs 54% in lobar or segmental infiltrates, p>amp;lt;0.001).
      
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Twenty-seven specimens collected from abdominal cavities or incisions on the abdominal wall of patients with suppurative infection were examined bacteriologi-cally, with the main purpose of isolating and identifying the non-sporc-forming anaerobes. Twenty-eight strains of aerobes (including facultative anaerobes) and twenty-two strains of obligate anaerobes were screened out. 90.9% of the obligate anaerobes were bacteroids and 64% of the aerobes were G bacteria. Bacterial cultures showed that the infection rates...

Twenty-seven specimens collected from abdominal cavities or incisions on the abdominal wall of patients with suppurative infection were examined bacteriologi-cally, with the main purpose of isolating and identifying the non-sporc-forming anaerobes. Twenty-eight strains of aerobes (including facultative anaerobes) and twenty-two strains of obligate anaerobes were screened out. 90.9% of the obligate anaerobes were bacteroids and 64% of the aerobes were G bacteria. Bacterial cultures showed that the infection rates of aerobes and the mixture of them were 55.6%, 18.5% and 25.9% respectively. The total infection rate of anaerobes was 44.4%. The rate of anaerobes isolated from patients with suppurative infection after appendectomy (58.3%) was the highest among the specimens. These results signify that anaerobes are significantly important pathogens in abdominal suppurative infection.

收集27份腹腔内和腹壁切口感染脓汁标本,进行以无芽胞厌氧菌为重点的细菌学分离鉴定。其中共检出需氧菌(含兼性厌氧菌)28株和专性厌氧菌22株。厌氧菌中类杆菌属占90.9%。细菌培养结果表明,单独需氧菌感染占55.6%,单独厌氧菌感染占18.5%,需氧菌和厌氧菌混合感染占25.9%。厌氧菌的总感染率为44.4%。几种不同标本中,以阑尾炎手术后化脓感染检出厌氧菌的比率最高,占58.3%。这一结果表明,厌氧菌在腹部化脓感染中,占有不可忽视的地位。

Short chain fatty acids(SCFA)produced by 60 anaerobic bacteria are analysed bygas chromatography.It showed that SCFA produced by anaerobic bacterial pure culturehad great significance in identification,and especially in separation of genus,suchas bacteroides,clostridia,fusobacteria,peptostreptococci and gram-positive anaerobicrods.Twenty SCFA detected were compared with the conventional cultural method.The detection of propionic,isobutyric,butyric,isovalaric,valaric and caproic acidsfavors the diagnosis of anaerobic...

Short chain fatty acids(SCFA)produced by 60 anaerobic bacteria are analysed bygas chromatography.It showed that SCFA produced by anaerobic bacterial pure culturehad great significance in identification,and especially in separation of genus,suchas bacteroides,clostridia,fusobacteria,peptostreptococci and gram-positive anaerobicrods.Twenty SCFA detected were compared with the conventional cultural method.The detection of propionic,isobutyric,butyric,isovalaric,valaric and caproic acidsfavors the diagnosis of anaerobic infections.This is a rapid method for the preliminarydifferentiation of anaerobic and aerobic infections.

本文采用国产102G型层析仪测定了1981年10月至1982年12月分离得的60株厌氧菌在培养液中的脂肪酸代谢产物,还直接测定27份脓液标本中的细菌代谢产物,并和细菌培养结果进行对照。通过该测定较易对一些生化反应难以鉴定的厌氧菌作出分属和鉴定,并证实脓液中如有乙酸、丙酸加上异丁酸、丁酸、异戊酸、戊酸、异己酸和己酸中任何一种者,即可初步诊断为厌氧菌(尤其是拟杆菌和梭形杆菌)感染。本在收到标本1小时内即可初步作出厌氧菌感染的诊断,对指导临床用药、提高治愈率有重要价值。

In this article, the results of anaerobic bacterial culture from subgingival plaques of 51 cases with periodontal disease, 16 cases with gingivitis, and 9 normal subjects were reported. In 51 specimens taken from patients with periodontal disease, 146 bacteria colonies were developed, of whith 111 (76%) were anaerobic organisms and 35 (24%) were faculative anaerobic organisms. Of the anaerobic organisms, 59 colonies (53%) were Gram-negative anaerobic organism, mostly anaerobic rods (54/59), including 29 colonies...

In this article, the results of anaerobic bacterial culture from subgingival plaques of 51 cases with periodontal disease, 16 cases with gingivitis, and 9 normal subjects were reported. In 51 specimens taken from patients with periodontal disease, 146 bacteria colonies were developed, of whith 111 (76%) were anaerobic organisms and 35 (24%) were faculative anaerobic organisms. Of the anaerobic organisms, 59 colonies (53%) were Gram-negative anaerobic organism, mostly anaerobic rods (54/59), including 29 colonies of bacteroides and 25 colonies of fusobacterium. The authors believe that there may be a close relationship between periodontal disease and Gram-negative anaerobic organism.

报道牙周病、牙龈炎及正常牙龈龈下菌斑厌氧菌培养的结果。51例牙周病标本检出146株,其中厌氧菌111株,兼性厌氧菌35株。厌氧菌中革兰氏阴性厌氧菌59株,革兰氏阴性厌氧菌中革兰氏阴性厌氧杆菌占优势,其中类杆菌属29株、梭形杆菌属25株。类杆菌属中又以产黑色素类杆菌菌株居多,梭形杆菌属中核梭形杆菌居多为22株,而其他菌株数则较少,我们认为革兰氏阴性厌氧杆菌与牙周病的关系是比较密切的。

 
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