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coli culture results in oscillations of the topological state of DNA.
      
However, incubation of isolated macrophages in the presence of dextran sulfate and such medium transfer into hepatocyte culture results in even more pronounced increase in TAT activity.
      
The addition of a carbon and energy source to this culture results in a second stress reaction.
      
The culture results showed that Spirulina platensis grows successfully in diluted human urine, and yields maximal biomass at urine dilution ratios of 140~240.
      
Furthermore the culture results were related to the radiographic extension of pulmonary infiltrates (92% positive cultures in multilobarvs 54% in lobar or segmental infiltrates, p>amp;lt;0.001).
      
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We have studied the normal value of GM-CFU-c in bone marrow in children with non-cynotic type of congenital heart diseases and with blood disorders, such as acute leukemia, splastic anemia, idiopathic thrombocytopenia and hypersplenism etc, as well as normal adults using the technique of double-layer soft agar culture in vitro in this paper.The results showed the number of GM-CFU-c reduced significantly, and even any growing of GM-CFU-c in relapse in cases with acute leukemia and severe type of aplastic amemia....

We have studied the normal value of GM-CFU-c in bone marrow in children with non-cynotic type of congenital heart diseases and with blood disorders, such as acute leukemia, splastic anemia, idiopathic thrombocytopenia and hypersplenism etc, as well as normal adults using the technique of double-layer soft agar culture in vitro in this paper.The results showed the number of GM-CFU-c reduced significantly, and even any growing of GM-CFU-c in relapse in cases with acute leukemia and severe type of aplastic amemia. We also found that even the patients with acute leukemia can get remission with the treatmeat of adriamycin and methotrexate, the disease will soon become relapse if the number of GM-CFU-c still remains in low level. All these results fully convinced that hemotopoietic stem cell culture in vitro has great advantages in predicting the prognosis of the diseases.

本文应用体外双层软琼脂培养技术,对末梢血象和骨髓象正常的非青紫型先天性心脏病患儿、正常成人骨髓及小儿血液系统疾病患者的骨髓,进行了单核—巨噬细胞集落(GM-CFU-c)形成能力的观察。结果21例非青紫型先心患儿,每2×10~5骨髓有核细胞可形成31~98个集落,均值为52.92±20.46,5例正常成人骨髓的GM-CFU-c数为42.5±8.43,经t检验,两者无明显差异,先心患儿可作为实验的正常对照组。作者观察了31例急性白血病,处于发作期的20例c}M—CFU-c数目均明显减少或不生长。11例完全缓解者的GM-CFU-c均在正常水平。另1例红白血病,在骨髓象确诊前5个月,多次随访,骨髓象仅示早期细胞轻度增加,但GM-CFU-c始终生长不佳,提示正常前体细胞已有增殖受抑。GM-GFU-c培养可有助于早期诊断和较早地预报白血病的复发。本组观察10例再障中大部份明显减少。在4例恶性肿瘤骨髓转移病例,GM—CFU-c数均显著低于正常值,另外5例原发性血小板减少性紫癜和2例脾功能亢进GM-CFU-c培养结果均在正常范围。实验表明,GM-CFU-c测定对造血系统疾病的诊断、治疗及预后判断具有一定的实用价值。

Objective:To dectect the canse,bacteria,sensitivity and treatment in the newbrons with ventilator\|associated pneumonia.Method:Analyse the diagnosis and treatments from 103 cases of newbrons with ventilator\|associated pneumonia.Result:Acinetobacter calcoaceticas bio anitratus,pseudomones aeruginosa and klebsiella pneumoniae were the most common bacteria.There was high resistibility to antibioties,but sensitive to tienam and ciprofloxacin.Conclusion:Gram negative bacteroid is the most important bacteria in the...

Objective:To dectect the canse,bacteria,sensitivity and treatment in the newbrons with ventilator\|associated pneumonia.Method:Analyse the diagnosis and treatments from 103 cases of newbrons with ventilator\|associated pneumonia.Result:Acinetobacter calcoaceticas bio anitratus,pseudomones aeruginosa and klebsiella pneumoniae were the most common bacteria.There was high resistibility to antibioties,but sensitive to tienam and ciprofloxacin.Conclusion:Gram negative bacteroid is the most important bacteria in the newborns with ventilator\|associated pneumonia and has high resistibility.It should be prevented and treated carefally.

目的:探讨新生儿通气相关性肺炎病因、病原菌、耐药情况、相关防治措施。方法:分析103 例新生儿通气相关性肺炎患儿痰培养结果、诊治情况。结果:醋酸不动杆菌、铜绿假单胞菌、肺炎克雷伯杆菌是三种主要致病菌,耐药率高,对泰能、环丙沙星最为敏感。结论:革兰阴性杆菌是新生儿通气相关性肺炎重要病原菌,耐药率高,治疗较为困难,应积极预防。

Objective To study the etiological change of neonatal septicemia and direct clinic treatment.Methods The patients with neonatal septicemia were divided into two groups,1983~1988 and 1993~1998.Compare clinic manifestations ,blood culture,and drug sensitivity by statistical methods.Results Infection in hospital was primary in 80s group(80 77%).Infection out hospital made up 50 percent in 90s group.Skin,umbilicus,respiratory tract were primary route of infection.Abnormal temperature,feeding difficulty,listlessness,jaundice,umbilical...

Objective To study the etiological change of neonatal septicemia and direct clinic treatment.Methods The patients with neonatal septicemia were divided into two groups,1983~1988 and 1993~1998.Compare clinic manifestations ,blood culture,and drug sensitivity by statistical methods.Results Infection in hospital was primary in 80s group(80 77%).Infection out hospital made up 50 percent in 90s group.Skin,umbilicus,respiratory tract were primary route of infection.Abnormal temperature,feeding difficulty,listlessness,jaundice,umbilical inflammation were common clinical features.Staphylococcus epidermidis and staphylococcus aureus were primary pathogenic bacteria.MRSE infection rose in 90s group.Penicillin,erythromycin and ampicillin were lower sensitivity,but cephalosporin was higher. Conclusion Treatment and prevention of infection in hospital were primary measure that reduced neonatal septicemia.The antibacterial with anti-β-lactanase was higher sensitivity

目的探讨近10年新生儿败血症病原学的变化特点。方法比较80年代组与90年代组新生儿败血症的临床表现、血培养结果、药敏结果的异同点。结果80年代组以院外感染为主(占8077%),90年代组院内感染增加达5000%;皮肤、脐部及呼吸道感染始终是主要的感染途径。体温异常、喂养困难、精神差及黄疸、脐部炎症是两组均常见的临床表现。表皮葡萄球菌、金黄色葡萄球菌是主要的致病菌,90年代耐甲氧西林金黄色葡萄球菌感染率明显上升。青霉素、红霉素及氨苄青霉素敏感率较低;含β内酰胺酶抑制剂的抗生素(优力欣,头孢二、三代)敏感性较高。结论防治医院内获得性感染,是减少新生儿败血症发生的主要措施;随着致病菌的变化,含β内酰胺酶抑制剂的抗生素敏感性较高。

 
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