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     The genetic engineering expression bacterial hIL13pGEX4T2/TG1 was successfully constructed through the insertion of hIL13 fragment(0.3 kb) that was directly amplified from activated healthy human peripheral blood mononuclear cells(HPBMC) by RTPCR method,into the GST fusion expression vector pGEX4T2,and the transformation into competent E.
     通过RTPCR技术直接从激活的健康人外周血单个核细胞(HPBMC)中扩增出约03kb的hIL13基因片段,经DNA重组技术,插入到融合蛋白表达载体pGEX4T2中,转化入感受态的大肠杆菌TG1中,成功地构建了hIL13的基因工程表达菌株hIL13pGEX4T2/TG1。
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     Methods: RTPCR technique was used to measure the levels of MMP2 mRNA and TIMP2 mRNA in 12 cases of colon carcinoma and 20 cases of rectal carcinoma (involved 4 recurrent cases).
     方法:采用RTPCR方法对12例结肠癌、20例直肠癌(其中4例为复发癌)组织及相应正常组织中MMP2和TIMP2mRNA表达水平进行测定。
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     Methods The expression of mdr1 and MRP were measured in 55 patients with AL by reverse transcription polymerase chain reaction (RT PCR).
     方法采用逆转录聚合酶链反应(RTPCR)的方法检测55例AL患者的mdr1及MRP的表达。
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     Expression of TGF β 1 mRNA was evaluated by RT PCR.
     RTPCR方法测定肺组织内TGFβ1的表达。
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     mRNA expression of β Actin, CD40L, bcl xL, TGF β 1, Fas, FasL, Perforin and Granzyme B was detected by using RT PCR technique in the samples of CD28 costimulated lymphocytes.
     以逆转录聚合酶链反应(RTPCR) 检测βActin、CD40L、bclxL、TGFβ1 、Fas、FasL、穿孔素和颗粒酶BmRNA 表达水平。
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  “rt”译为未确定词的双语例句
     Methods Using the quantitative reverse transcription PCR procedure,we observed the expression of nm23 mRNA in human pancreatic cancer JF305 cell sublines with different metastatic potential.
     方法 在建立不同转移潜能的人胰腺癌JF305 单克隆细胞亚系的基础上,应用定量逆转录多聚酶链式反应技术(RTPCR)检测不同转移潜能的人胰腺癌JF305 单克隆细胞亚系细胞中nm23 mRNA 的表达。
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     Results Expression of ET 1 mRNA and TNFα mRNA from renal tubular epithelial cells was obtained (546 bp and 415 bp) respectively as well as the relevant ET 1 and TNFα proteins.
     结果 肾小管上皮细胞既有ET1mRNA和TNFαmRNA 的表达(RTPCR的扩增片段分别为546 bp 和415 bp) ,还有ET1 及TNFα的蛋白合成及分泌;
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     PMLRARα was found by PTPCR in all patients, 8 of them had PMLRARα longtype fusion(L) and 5 had short ones.
     RTPCR均发现有PMLRARα融合基因,8例为长型(L)融合,5例为短型融合。
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     Method : Exogenous human I L 10(h I L 10) gene wastransferred into rat C M Cby retroviral vector p L X( I L 10) S N. The polymerase chain reaction ( P C R) ,the reverse tran scription polymerase chain reaction ( R T P C R) and E L I S A were used ①to detect integration and expression of h I L 10gene in rat C M C;
     方法:通过重组逆转录病毒载体p L X( I L10) S N 将人 I L10 基因导入大鼠 C M C,应用 P C R, R T P C R 和 E L I S A:①检测 I L10 基因的整合和表达;
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     The complete nucleoprotein(NP)gene of an avian influenza virus isolate A/Chichen/Xinjiang/1/96(H14N5)(XJ)was sequenced.
     对RTPCR扩增的禽流感病毒A/Chicken/Xinjiang/1/96(H14N5)分离株核蛋白基因进行了序列分析。
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     The result of immnohistochemical staining was almost similar to that by RT PCR method.
     结果接近RTPCR 法;
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     3. In the regions of RT% VDB and MSN , the different changes of the positive expression of Dl receptor were observed, but still had no statistical difference.
     3.在RT
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     Gly.
     Gly。
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     The kinetic equation may be expressed as:dα/dt=A·e~(-E/RT)·3/2\~(-1).
     e-E/RT.
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     The cytokines expression and secretion of these TCC were analysed by ELISA and RTPCR.
     用RTPCR及ELISA检测其细胞因子的表达和分泌。
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  rt
Efavirenz is a trifluoromethylated inhibitor of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) that shows good results in anti-HIV chemotherapy.
      
With the objective of developing efficient non-nucleoside compounds, we have designed and synthesized some new 3-hydroxy-2-oxo-3-trifluoromethylindole as potential RT inhibitors.
      
We have used molecular docking with HIV-1 RT as a tool to design putative non-nucleoside reverse transcriptase inhibitors (NNRTIs).
      
Based on the calculation results obtained, a series of new 3-hydroxy-2-oxo-3-trifluoromethylindoles were synthesized as a novel class of potential RT inhibitors.
      
glandulosa was analyzed by a semi-quantitative RT-PCR.
      
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The viscosities of the binary solutions of n-butyl alcohol and N-ethylanilinehave been measured at 15.00℃ in an Ubbelohde viscometer.The fluidities of n-butyl alcohol and N-ethylaniline were found to be 29.61 and 39.05 rhes respec-tively,while the solution at ca.30 mole percent butyl alcohol shows a maximumfluidity of 41.10 rhes.This increase of fluidity probably results from the break-ing of association interactions between the hydroxyl bonds of butyl alcoholmolecules in the solution.Deviations from the linear...

The viscosities of the binary solutions of n-butyl alcohol and N-ethylanilinehave been measured at 15.00℃ in an Ubbelohde viscometer.The fluidities of n-butyl alcohol and N-ethylaniline were found to be 29.61 and 39.05 rhes respec-tively,while the solution at ca.30 mole percent butyl alcohol shows a maximumfluidity of 41.10 rhes.This increase of fluidity probably results from the break-ing of association interactions between the hydroxyl bonds of butyl alcoholmolecules in the solution.Deviations from the linear additivity of fluidities arepositive for all concentrations.The experimental data can be represented fairlywell by an equation similar to that proposed by Eyring,i.e.φ=((?)/Nh)exp(-(x_1△F_1+x_2△F_2+Bx_1x_2/RT))with the parameter B=-374 cal/mole,and equally well by the equation of vander Wyk:logφ=x_1~2 logφ_1+x_2~2logφ_2+B'x_1x_2with the parameter B'=3.363.

正丁醇—N-乙基苯胺雨元溶液在15.00℃时的粘度,已被测定。这溶液的流度在正丁醇的分子浓度是30%时,呈现一最大值。溶液的流度增高,可能是由于正丁醇分子聚合的离解所致。实验数据可以相当满意地用类似 Eyirng 所提出的雨元溶液粘度公式或 van der Wyk 的公式来表示。

Using the x,y,p coordinates,and neglecting the small terms in thesteady state vorticity equation, we have(?)taking two layer baroclinic model,as Sawyer and Bushby,we let(?)in whichA(p)=(p_0+p_1-2p)/(p_0-p_1),(3)where p_0=1000mb and p_1=200mb.Introducing (2) to (1) and integrating from p=p_0 to p=p_1 under thefollowing bundary conditions(?)We obtain(?)where H=(RT_0)/g is the height of the homogeneous atmosphere,and η=η(x,y) is the topography of the earth's surface.For the distribution of vertical velocity,we...

Using the x,y,p coordinates,and neglecting the small terms in thesteady state vorticity equation, we have(?)taking two layer baroclinic model,as Sawyer and Bushby,we let(?)in whichA(p)=(p_0+p_1-2p)/(p_0-p_1),(3)where p_0=1000mb and p_1=200mb.Introducing (2) to (1) and integrating from p=p_0 to p=p_1 under thefollowing bundary conditions(?)We obtain(?)where H=(RT_0)/g is the height of the homogeneous atmosphere,and η=η(x,y) is the topography of the earth's surface.For the distribution of vertical velocity,we assume ω(x,y,p)=B(p) (?)(x,y)+C(p) ω_0(x,y),(5)whereB(p)=1-A(p)~2,C(p)=(p-p_1)/(p_0-p_1).(6)Introducing (2) and (5) to (1),multiplying A(p),and then integratingit with respect to p from p_0 to p_1,we find(?)Using again the relations of (2) and (5) and the geostrophic approximationin (Ⅰ)(1),taking steady state and then eliminating (?) from it with (10),we have(?)whereC_1=[R/(gc_p)]ln[p_0/(?)],M=[(4f~2)/(p_0-p_1)][1/(C_2RΓ_p)],C_2=(1/2)[(p_0+3p_1)/(p_0-p_1)]-[(4p_0p_1)/(p_0-p_1)~2]ln[(2p_0)/(p_0+p_1)].The motion is considered as consisting of small perturbations superim-posed upon a basic zonal current (?)+A(p)U_T,and taking geostrophic app-roximation,wo obtained the two required equations:(?)in which β is the variation of Coriolis parameter with latitude.We used the equation (9) and (10) to a rectangular reigon with a mar-ginal cyclicity and with a length equal to that of the latitud circle at 45°and take 1 day as the time unit and the radius of 45° latitude circle aslength unit.By means of Fourier analysis,the solution may be written inthe following form(?)where the topographical influence function Φ_0 and the heating influencefunction Φ_h are respectively defined by the Fourier series (?)As the equation was linearzed, we can put Q_m=0 to find the topographiceffect, and put η=0 to find the effect of heat sources and sinks;whenboth Q_m≠0 and η≠0,we can find the combined influences of heat sour-ces and sinks and topography.For case of winter,we take (?)=15m/s and U_t=13m/s.In the unitsdescribed above,we find (?)=2.87×10~(-1),β=2π,M=231,β/(?)=21.9.We have constructed separately the flow patterns respectively due tothe thermal effect,the topographical effect and the combined effect of themon the westerlies.By examining these patterns we may state the followingpoints on the formation of mean troughs and ridges and jet stream:(1)For the case Q_m=0.There is a trough to the downstream ofmountains and a ridge over the mountains created by the forced ascent ofthe westerly current over the topography.The Tibetan Platean is importanton the formation of the Asiatic mean troughs and ridges.(fig.1) Consi-dering the combined effect of the mountains of Rocky and Greenland,theposition and intensity of the computed mean trough and ridge (fig.6) iswell in agreement with the observation.(fig.4,10) However the effect ofthe forced ascent of the westerly current over the mountains does notgive a jet stream.(2)For the case η=0.Through dynamic processes the large-scaleheat sources and sinks show even larger influences than that of the moun-tains on the midtropospheric flow.The 500 mb trough is located west ofthe heat source,and the ridge west of the heat sink.The jet stream isformed at the south-western part of the heat source.The perturbationpattern of heating and cooling (fig.2,7) is in better agreement with theobserved one than that due to topography.(3)The flow pattern obtained by considering both heat sources andsinks and topography (fig.3,9) is in good agreement with the observedones.The agreement with observation is much better than any one of thefactors considered alone.

本文第二部分根据简单的两层斜压模式,用小扰动分别解出地形、热源的常定扰动和地形与热源的共同扰动.作者得到西藏高原对于东亚平均槽、脊的形成具有一定的作用.洛矶山加上格林兰高地后更能解释北美槽、脊的形成.在夏季由于西风带的北移,只有地形作用也使得平均槽的强度和位置发生变化.热源的扰动作用对平均槽的生成具有更显著的作用,冬季的西风急流和夏季小槽的生成都和热源扰动更有直接的关系.考虑了热源和地形的共同作用后所得的扰动流型更接近了实现.由此作者提出热源和地形作用通过动力过程在大气环流中相互制约的统一性.

Taking two layer baroclinic model,as given by Sawyer and Bushby,and introducing the topographical effect,we obtain the following equationsfrom the linearized vorticity equation and adiabatic equation(?)z+βz+U_T/3(?)h=-f~2/gH((?)-U_T)η,(?)h+U_T(?)z+(?)(β/(?)-M)h+MU_Tz=0,where η=η(x,y) is the topography of the earth's surface,H=RT_0/g,andM=4f~2/p_0-p_1 1/C_2RΓ_p,C_2=p_0+3p_1/p_0-p_1-4p_0p_1/(p_0-p_1)~2ln2p_0/p_0+p_1,Γ_p=r_d/ρg-(?),in which p_0=1000mb,p_1=200mb.By means of Fourier analysis,the solution of...

Taking two layer baroclinic model,as given by Sawyer and Bushby,and introducing the topographical effect,we obtain the following equationsfrom the linearized vorticity equation and adiabatic equation(?)z+βz+U_T/3(?)h=-f~2/gH((?)-U_T)η,(?)h+U_T(?)z+(?)(β/(?)-M)h+MU_Tz=0,where η=η(x,y) is the topography of the earth's surface,H=RT_0/g,andM=4f~2/p_0-p_1 1/C_2RΓ_p,C_2=p_0+3p_1/p_0-p_1-4p_0p_1/(p_0-p_1)~2ln2p_0/p_0+p_1,Γ_p=r_d/ρg-(?),in which p_0=1000mb,p_1=200mb.By means of Fourier analysis,the solution of the perturbation thicknessof 1000—500mb (h) may be written in the following formh(x,y)=f~2/gH 3U_T((?)-U_T)/3(?)~2-U_T~2 integral form 0 to 2π integal from 0 to 2π η(α,γ)I_0(x-α,y-γ)dαdγ,where the influence function I_0 is given by the double seriesI_0(x,y)=1/4π~2(?)M-(λ~2+k~2)/(λ~2+k~2)+2M(λ~2+k~2)-3M/2 β/(?)The numerical results of winter ((?)=15m/s,U_T=13m/s) are given bythe figures in the text.

本文根据两层斜压模式得到大尺度地形对1000—500mb 平均温度场的常定扰动.结果得到大地形对温度槽脊的形成具有一定的作用,而地形所造成的高度槽在垂直方向上没有倾斜现象.

 
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