Results The nucleotide sequence of L PGDS cDNA in human testis has 99 5% homology with gene sequence coding L PGDS in human peripheral blood lymphocytes and 96 0% with L PGDS cDNA in human brain. The homology of corresponding amino acid sequences are 100% and 94 2% respectively.
结果 人睾丸 L - PGDS c DNA核苷酸序列与人外周血淋巴细胞的 L - PGDS基因编码区序列的一致性为 99.5 % ,与人脑 L -PGDS c DNA的一致性为 96 .0 % ,相应氨基酸的一致性分别为 10 0 %和 94.2 %。
Cytogenetic effect of fluoride by short culture of human peripheral blood lymphocytes showed that fluoride can significantly increase the frequencies of SCE (P<0.01), but can not significantly induce chromosomal aberration and lagging of cell cycle(P>0.05).
Results In vivo, the leukocytes were stimulated to increase by super antigen products at the dose of 4ng/ml or more(P<0 01), and the spleen cells and human peripheral blood lymphocytes were proliferated by super antigen products at the dose more than 5ng/ml, Which were better than the positive control (P<0 01).
The results of biological activity showed that TAF-Ⅰ specifically increased E-rosette formation of human peripheral blood lymphocytes and increased the proliferative respond of the mitogen ConA-induced mice splenocytes.
PNIP could significantly suppress the proliferation of the lymphocytes from human peripheral blood and C57BL/6J mouse spleen and the malignant lymphoid cells, L5178 cells and Raji cells, but had no effect on Hela cells and Vero-E6 cells which are nonlymphoid cells.
The Na-PCP and dioxins(2,3,7,8-TCDD) can damage DNA of lymphocyte from human peripheral blood,and the damage will rise with the increase of their mass concentrations.
The comet assay and micronucleus assay were determined for lymphocyte from human peripheral blood,which is infected by Na-PCP and dioxins(2,3,7,8TCDD).
The data on spontaneous chromosome aberration rates in cultures of human peripheral blood lymphocytes obtained in the past 30 years have been collected to form a database.
Incubation of human peripheral blood lymphocytes with zinc transferrin (with or without phytohemagglutinin) induces the synthesis of protein that elutes from a Sephadex G-75 column at aVe/Vo value corresponding to a molecular weight of 6600.