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结合图像
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  and image
     Methods By using immunochemical method and image analysis technique, nm23-H1 protein expression was quantitatively determined in44 cases of fibrocystic disease of breast (FCD) which was subdivided into 3 grades according to Page'scriterion, 28 cases of invasive ductural carcinoma of breast (IDC) and 5 cases of human normal breasttissue (NMB).
     方法应用免疫组化方法结合图像分析技术对根据Page标准分为三级的44例乳腺增生症(FCD)、5例正常乳腺组织(NMB)及28例乳腺浸润性导管瘤(IDC)的nm23-H1蛋白表达进行定量测定。
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     Methods The sciatic nerve ligation was made in 42 SD rats. On day 1, 3, 5, 7, 14, 21 and 28 after ligation, the expression of c-Met was detected by immunohistochemical method and image analysis technique.
     方法采用免疫组织化学方法结合图像分析技术检测大鼠坐骨神经结扎后1、3、5、7、14、21和28d c-Met在脊髓和DRG内的表达变化情况。
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     TGF β 1 contents were assessed by immunohistochemical method and image analysis technique in 55 pieces of human bladder cancer tissue, 20 bladder tissue adjoining the neoplastic area and 15 normal bladder tissue.
     为探讨膀胱移行细胞癌中转化生长因子β1(TGFβ1)含量及其价值,采用免疫组化结合图像分析技术对55例膀胱癌,20例癌旁组织和15例正常膀胱组织TGFβ1表达进行检测,结合临床资料分析。
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     The counts and mean area of GFAP positve cells in rats' hippocampal CA1, CA3 and DG areas in the 4 groups were measured by immunohistochemical technique and image analytical system, so as to quantitatively analyze the influence of ethanol on the structure and function of hippocampus and dentate gyrus, and the role of anisodamine.
     采用免疫组化技术并结合图像分析系统,测定海马CA1,CA3,DG区胶质纤维酸性蛋白表达阳性的胶质细胞的计数和平均面积百分比,以定量分析乙醇对海马及齿状回结构和功能的影响及山莨菪碱的作用。
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     Methods Immunohistochemical ABC method and image analysis were used to investigate the expression and content of NDR2 in human normal thyroid and carcinoma biopsies.
     方法:随机收集正常甲状腺及乳头状甲状腺癌病理标本,采用小鼠抗NDR2单克隆抗体的免疫组织化学ABC法,研究NDR2蛋白在人正常甲状腺及甲状腺乳头状腺癌中的表达,并结合图像分析比较NDR2蛋白在正常甲状腺组织与癌组织中含量的变化。
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     After hygromycin screening and cloning FCM, TRAP-PCR-ELISA and immunohistochemical staining (IHC) in combination with imaging analysis were used to determine simultaneously activity of SMMC7721/pBBS212-hTR telomerase and P16, P21 expressions.
     细胞克隆转移扩大培养后,采用流式细胞仪、TRAP-PCR-ELISA及免疫组化技术,结合图像分析,在检测SMMC7721/pBBS212-hTR细胞端粒酶活性的同时,检测P16和P21的表达。
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     Cyclin D1 protein expression in the Huh 7 cells treated with 100 ng/ml leptin was also significantly increased compared with the controls(P<0.01) while the P21waf1 protein expression was suppressed(P<0.01).
     免疫细胞化学染色结合图像分析系统分析也发现100 ng/ml的瘦素处理24 h后,与对照组相比,Cyclin D1蛋白的表达明显增高(P<0.01),P21waf1蛋白的表达明显降低(P<0.01)。
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     Methods Expression of FAK,integrin α 5β 1,E-cadherin and the phosphorylation of FAK was examined in SMMC-7721 cell by Western blot analysis,flow cytometry,immunohistochemistry assay(ABC method).
     方法 采用免疫组化ABC法 ,结合图像分析仪及流式细胞术和Western印迹方法测定SMMC 772 1细胞表面E 钙粘蛋白 (E cadherin)、α5β1整合蛋白 (α5β1intergrin)、粘着斑激酶 (FAK )表达及粘着斑激酶磷酸化的程度。
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     Methods:Rats were divided randomly into 5 groups: saline group,sodium glutamate group,IL-1β+GluNa group,rhIL-1ra(human recombined interleukin receptor antagonist)+IL-1β+GluNa group and D-AP-5 (noncompetitive NMDA receptor antagonist)+IL-1β+GluNa group. Immunohistochemistry combined with the image quantitative analysis were used.
     方法 :把大鼠随机分为生理盐水组、GluNa组、IL 1 β +GluNa组、人重组IL 1受体拮抗剂 (rhIL 1ra) +IL 1 β +GluNa组及D AP 5 (非竞争性NMDA受体拮抗剂 ) +IL 1 β+GluNa ,利用免疫组织化学并结合图像分析进行研究。
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     The integra optical density(IOD) of MMP-2and TIMP-2in the tumor tissu es and the ratio of the MMP-2to the T IMP-2we re detected semi-quantitatively by immunohistochemical staining comb ined with image analysis.
     免疫组化染色结合图像分析系统半定量检测移植瘤组织内MMP-2、TIMP-2积分光密度(IOD)及MMP-2/TIMP-2比值。
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  相似匹配句对
     Combining with B. G.
     结合B.G.
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     Referring to the self-guard mechanism of G.
     结合G.
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     Image Binarization Based on Canny's Operator
     结合Canny算子的图像二值化
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     Research on Coding of Compound Images
     动静态结合图像编码
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     Image Compression Technology
     图像压缩技术
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  and image
An image-tracking algorithm based on object center distance-weighting and image feature recognition
      
A novel method of plotting sensor sensitivity field and image reconstruction algorithm for electrical capacitance tomography sys
      
The camera is set on a mobile carrier, and image registration is conducted by a matrix photoreceiver on basis of charge-coupled devices (CCD) in delay-integration mode.
      
The Role of Virtual Receptor Structures in the Formation of Retinal Signals and Image Perception
      
The mechanism responsible for the revealed effect is proposed, and image recording is accomplished.
      
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Cholinesterase histochemis-try combined with the technique of image analysis was used to investigate the effects of clo-nidine,a centrally active alpha-2 adrenergic agonist,on the kinetic course of soman poisoning.The results showed that clonidine didn't affect maximal extent of ChE inhibition by soman,but greatly reduced inhibition rate.The kinetic process of ChE inhibition in cortexand medulla oblongata was strikingly modified,but much more mildly in basal ganglion.This probably suggests that soman toxicity...

Cholinesterase histochemis-try combined with the technique of image analysis was used to investigate the effects of clo-nidine,a centrally active alpha-2 adrenergic agonist,on the kinetic course of soman poisoning.The results showed that clonidine didn't affect maximal extent of ChE inhibition by soman,but greatly reduced inhibition rate.The kinetic process of ChE inhibition in cortexand medulla oblongata was strikingly modified,but much more mildly in basal ganglion.This probably suggests that soman toxicity is due mainly to its inhibition of ChE in cortex and medulla cblongata.

用ChE组织化学结合图像分析技术,探讨了可乐定对梭曼中毒动态过程的影响。结果可乐定对梭曼抑制ChE的最大抑制程度没有作用,而显著地降低了ChE抑制速率。脑组织的皮质和延脑区域ChE动态抑制过程受可乐定影响明显,而底节影响较轻,说明梭曼中毒的毒性表现主要由脑皮质和延脑ChE抑制所致。

Aging changes of tyrosine hydroxylase (TH) -immuno reactive neurons in the locus coeruleus were examined in young (3 month-old). adult(12 month-old) and aged(20 month-old) Wistar male rats, using the IGSS method and image quantitative analysis. The results obtained are as follows: ① The number of TH-immunoreactive neurons docreased markedly (P<0.01)in adult rats and docrcased further in old rats. The number of neurons compared with that in young rots declined by 70% in old rats. ② The area of TH-immunoreactive...

Aging changes of tyrosine hydroxylase (TH) -immuno reactive neurons in the locus coeruleus were examined in young (3 month-old). adult(12 month-old) and aged(20 month-old) Wistar male rats, using the IGSS method and image quantitative analysis. The results obtained are as follows: ① The number of TH-immunoreactive neurons docreased markedly (P<0.01)in adult rats and docrcased further in old rats. The number of neurons compared with that in young rots declined by 70% in old rats. ② The area of TH-immunoreactive neuronal somas increased with aging and had significant difference among separate groups (P<0.05). ③ The gray value of TH-immunoreactive neurons declined gradually from the young to the old and decreased by 39% in old rats. The results suggested that TH content and activity contained in neurons of the locus coeruleus declined continually with aging.

本实验用免疫金银染色法结合图像定量分析,观察了幼年(3个月)、中年(12个月)和老年(20个月)Wistar雄性大鼠蓝斑内酪氨酸羟化酶(TH)免疫反应性神经元的老年性变化,结果:蓝斑内TH免疫反应性神经元数量在中年呈显著性的减少(P<0.01),至老年,进一步减少,与幼年组比较,神经元丧失70%;TH免疲反应性神经元的胞体平均截面积随年龄增长呈增大趋势,各年龄组间有显著性差异(P<0.05);TH免疫反应性神经元的灰度值随增龄而递减,老年组与幼年组相比,下降了39%。表明蓝斑内儿茶酚胺能神经元所含TH的量和活性在衰老过程中是逐渐降低的,提示儿茶酚胺能神经递质合成能力减弱。

The content and distribution of TG in thyroid were determined by using BA-immunohistochemistry and image analyse; the protein spectrum and molecular weights of abnormal TG-related antigen proteins in the thyroid tissues were analysed with gradient PAGE and immunoblotting and the TG content in serum was measured by BA-ELISA. The experimental results showed: (1) The content of TG in thyroid of IHG homozygous goat group is significantly lower than that of control group (P<0.01). The TG in thyroid of normal goats...

The content and distribution of TG in thyroid were determined by using BA-immunohistochemistry and image analyse; the protein spectrum and molecular weights of abnormal TG-related antigen proteins in the thyroid tissues were analysed with gradient PAGE and immunoblotting and the TG content in serum was measured by BA-ELISA. The experimental results showed: (1) The content of TG in thyroid of IHG homozygous goat group is significantly lower than that of control group (P<0.01). The TG in thyroid of normal goats is mostly localized in the colloid and stained intensively, whereas TG in thyroid colloid of IHG homozygous goats is stained less intensively. There is a few TG in the proliferative epithelial cells. (2) The IHG homozygous goats have 2 kinds of abnormal TG-related portein with molecular weights of 331000 and 110000, but IHG heterozygous goats have only one kind of such protein with a molecular weight of 110000. (3) The TG content in serum of IHG heterozygous goat group is lower (P<0.05). These results mentioned above suggested that the thyroid of IHG goat is functionally defective in TG biosynthesis, especially in the polymerization of TG subunits, thus resulting in the thyroid hormones being synthesized insufficiently. The abnormal TG-related protein with a molecular weight of 110000 is a specific indication for eliminating the IHG heterozygous goats from the flock and the reduced level of TG in serum is one of biochemical indices for the same purpose.

应用BA-免疫组化结合图像分析测定了遗传性甲状腺肿病山羊(以下简称IHO)甲状腺组织中甲状腺球蛋白(TG)的含量和分布,用梯度-PAGE结合免疫印迹法分析了甲状腺组织中异常TG相关抗原蛋白的带谱及分子量;用BA-ELISA法测定了血清中TG含量.结果表明:(1)IHG纯合子病山羊甲状腺组织中TG含量明显低于对照组山羊(P<0.01),且TG在胶质中多为弱阳性,在增生上皮细胞内几乎为阴性;(2)IHO纯合子病山羊甲状腺组织中异常TG相关抗原蛋白为331000、110000,而IHG杂合子病山羊异常TG相关抗原蛋白为110000;(3)IHG杂合子病山羊血清中TG含量明显低于对照组(P<0.05).提示:IHG病山羊甲状腺激素合成不足是由于TG合成障碍,且其具体发病环节可能是TG亚单位(110000、331000相关TG抗原蛋白)进一步聚合为1200000的TG过程缺陷的结果;异常TG相关抗原蛋白110000,可作为净化IHG杂合子病山羊的特异性生化指标,其血清中TG含量降低,亦可作为剔除IHG杂合子山羊的生化指标之一.

 
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