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   鉴别与分类 在 园艺 分类中 的翻译结果: 查询用时:0.539秒
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鉴别与分类
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  identification and classification
    Making Template DNA in the Study of Identification and Classification of Banana Germplasm Based on AFLP Fingerprint
    香蕉种质资源的AFLP鉴别与分类中DNA模板的制备
短句来源
    Identification and classification of 35 banana varieties were studied based on AFLP analysis.
    采用AFLP技术,对35个香牙蕉品种(系)进行了鉴别与分类
短句来源
    Identification and Classification of Banana (Musa AAA Group Cavendish) Varieties by AFLP
    应用AFLP进行香牙蕉品种(系)的鉴别与分类
短句来源
    Studies on Identification and Classification of Cavendish Germsplasm Resources with AFLP Molecular Markers
    AFLP技术在香牙蕉种质资源鉴别与分类研究中的应用
短句来源
    Identification and Classification of Banana (Musa.AAA Group Cavendish) Varieties by AFLP
    应用AFLP进行香牙蕉品种(系)的鉴别与分类
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  identification and classification
Chromosome identification and classification of the lines will permit them to be models for genetic studies that can be used thereafter as promising "secondary gene pools" for the purpose of plant breeding.
      
Chromosome identification and classification of the lines makes it possible to use them in breeding programs and genetic studies.
      
Examples of identification and classification of signals in the presence of distortions, noises, and interference are given.
      
The problems of identification and classification of these objects are addressed.
      
Identification and classification of subspecies of Asian cultivated rice and their hybrids
      
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One of the key factors for AFLP marker is the successful preparation of HMW template DNA.In order to obtain pure DNA and clear AFLP fingerprint for the further study of identification and classification for banana,extraction of tem-plate DNA from different plant organs of banana was studied.The results showed that the HMW template DNA could be ob-tained from plant organs including white young roots,outspread young leaves and mature leaves.It was more convenient to choose young and mature leaves as plant...

One of the key factors for AFLP marker is the successful preparation of HMW template DNA.In order to obtain pure DNA and clear AFLP fingerprint for the further study of identification and classification for banana,extraction of tem-plate DNA from different plant organs of banana was studied.The results showed that the HMW template DNA could be ob-tained from plant organs including white young roots,outspread young leaves and mature leaves.It was more convenient to choose young and mature leaves as plant organs for the excellent quality genome DNA extraction.The results also showed that the HMW tem plate DNA obtained by using SDS and CTAB method were suitable for banana AFLP analy sis.The quantity of DNA extracted by using SDS method was two times as much as that by using CTAB method,but the degree of purity was low-er than using CTAB method.No difference of the molecular weight of DNA extracted by using SDS and CTAB methods was noted(all around16kb).

在运用AFLP技术进行香蕉种质资源的鉴别与分类时,成功的关键之一是HMW基因组DNA的成功制备和避免降解,为了获得高纯度的HMW香蕉模板DNA和清晰的AFLP指纹图谱,以粉蕉为试材进行了用不同方法从不同植物体部位提取香蕉DNA的研究。结果表明,以香蕉未展开的幼叶、成熟叶、新根为材料提取的DNA量足以进行AFLP分析,但考虑到取材的方便性,最好选用未展开的幼叶或成熟叶;用改进后的SDS、CTAB法均可以获得HMW基因组DNA和AFLP指纹图谱,SDS法(用氯仿异-戊醇溶液抽提3次)提取的DNA量将近是CTAB法提取的DNA量的2倍,但SDS法提取的DNA纯度不如CTAB法提取的高。两种方法提取的DNA片段大小一致,大约在16kb左右。

Identification and classification of 35 banana varieties were studied based on AFLP analysis. Two pair of primers (EcoRIACC+MseICAT and EcoRIACC+MseICAG) were screened from 64 pair of primers, and a total of 107 sites were amplified , among which 84 (78.5%) were polymorphic. The differentiated rate among 35 banana varieties were up to 100%. The polymorphic bands were different among them. The Dice's coefficient of the 35 varieties ranged from 0.71~1.00. The genetic relationship among Musa AAA Group Cavendish...

Identification and classification of 35 banana varieties were studied based on AFLP analysis. Two pair of primers (EcoRIACC+MseICAT and EcoRIACC+MseICAG) were screened from 64 pair of primers, and a total of 107 sites were amplified , among which 84 (78.5%) were polymorphic. The differentiated rate among 35 banana varieties were up to 100%. The polymorphic bands were different among them. The Dice's coefficient of the 35 varieties ranged from 0.71~1.00. The genetic relationship among Musa AAA Group Cavendish were relatively close. The results showed that the 35 Musa AAA Group Cavendish varieties could be divided into 6 subgroups, and three banana varieties (Jineiya, Pingguo ang Yangjiangai) from different countries are actually same. The results based on the molecular level could provide theoretical basis for the classification of Musa AAA Group Cavendish..

采用AFLP技术,对35个香牙蕉品种(系)进行了鉴别与分类。从AFLP试剂盒所提供的64对引物组合中选取两对引物组合EcoR I ACC+Msc I CAT和EcoR IACC+Msc I CAG对35份香牙蕉材料进行了AFLP分析,共得到扩增位点107个,其中多态性位点84个,多态性位点比例达到78.5%,对35个品种的区分率达到100%。各品种(系)多态性带数存在差别,多态性带数最多为海南红蕉(56条),多态性比例最高为0.666 7,可见其杂合程度较高;多态性带最少的为大丰1-1号(33条),其多态性比例为0.392 9。各品种(系)相互之间的相似系数介于0.71~1.00,表明香牙蕉品种(系)之间遗传关系相对较近。依相似系数0.88的水平,可以将供试的35个香牙蕉品种(单株)分为6个品种群。鉴别了3个引自不同国家的品种,几内亚、苹果以及阳江矮实际上为同一个香牙蕉品种。该研究结果基于AFLP分子标记,能从分子水平上反映香牙蕉地方品种之间的亲缘关系,可以作为香牙蕉品种(系)分类的依据。

>=Identification and classification of 35 banana varieties were studied based on AFLP analysis . Two pair of primers (EcoRIACC + MselCAT and EcoRIACC + MselCAG were screened from 64 pair of primers, and a total of 107 sites were amplified , among which 84 (78.5%) were polymorphic. The differentiate rate among 35 banana varieties were up to 100% . The polymorphic bands were different among them. The Dice' s coefficient of the 35 varieties ranged from 0.71 - 1.00. The genetic relationship a-mong Musa AAA Group...

>=Identification and classification of 35 banana varieties were studied based on AFLP analysis . Two pair of primers (EcoRIACC + MselCAT and EcoRIACC + MselCAG were screened from 64 pair of primers, and a total of 107 sites were amplified , among which 84 (78.5%) were polymorphic. The differentiate rate among 35 banana varieties were up to 100% . The polymorphic bands were different among them. The Dice' s coefficient of the 35 varieties ranged from 0.71 - 1.00. The genetic relationship a-mong Musa AAA Group Cavendish were relatively close. The results showed that the 35 Musa AAA Group Cavendish varieties could be divided into 6 subgroups, and three banana varieties (Jineiya, Pingguo ang Yangjiangai) from different countries are autually same. The results based on the molecular level could provide theoretical basis for the classification of Musa AAA Group Cavendish.

采用AFLP技术,对35个香牙蕉(Musa.AAA Group Cavendish)品种(系)进行了鉴别与分类。从AFLP试剂盒所提供的64对引物组合中选取两对引物组合EcoRIACC+MseICAT和 EcoRIACC+MseICAG对35份香牙蕉材料进行了AFLP分析,共得到扩增位点107个,其中多态性位点84个,多态性位点比例达到78.5%,对35个品种的区分率达到100%。各品种(系)多态性带数存在差别,多态性带数最多为海南红蕉(56条),多态性比例最高为0.6667,可见其杂合程度较高;多态性带最少的为大丰1-1号(33条),其多态性比例为0.3929。各品种(系)相互之间其相似系数介于0.71~1.00,表明香牙蕉品种(系)之间遗传关系相对较近。依相似系数 0.88的水平,可以将供试的35个香牙蕉品种(单株)分为6个品种群。鉴别了3个引自不同国家的品种:几内亚、苹果以及阳江矮实际上为同一个香牙蕉品种。本研究结果基于AFLP分子标记,能从分子水平上反映香牙蕉地方品种之间的亲缘关系,可以作为香牙蕉品种(系)分类的依据。

 
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