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  protein
     THE CLINICAL SIGNIFICANCE OF C-REACTIVE PROTEIN (CRP) DETERMINATI0N IN ACUTE INFECTIOUS DISEASES
     检测急性传染病患者血清中C-反应蛋白(CRP)的临床意义
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     Reconstitution of Rabbit Brain GABA Binding Protein(Receptor)on Xenopus Oocytes
     兔脑γ-氨基丁酸结合蛋白(受体)在非洲爪蟾卵母细胞上的重建
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     Assayand Analysis of Antibody Level of Anti-endotoxiN Protein of Pseudomonas Aeruginosa Between healthy Person and Patients
     健康人和感染患者抗绿脓杆菌内毒素蛋白(EP)抗体水平的测定分析
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     Studies on Human Chorionic Gonadotropin (HCG)-binding Protein from Pseudomonas maltophilia by Ligand Blotting Assay
     嗜麦芽假单胞菌HCG结合蛋白(受体)配体印迹分析
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     THE SYNTHESIS, LOCALIZATION AND PROCESSING OF FOREIGN PROTEIN (BgLS) IN ESCHERICHIA COLI
     外源蛋白(BglS)在大肠杆菌中的合成、定域和加工
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  “蛋白(”译为未确定词的双语例句
     Analysis of HSPs of Male Sterile Sorghum by Electrophoresis
     高粱热激蛋白(HSPs)的电泳分析与雄性不育性
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     EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN MAMMALIAN CELLS USING HUMAN METALLOTHIONEIN PROMOTER AND BOVINE PAPILLOMA VIRUS
     利用人摄金蛋白(METALLOTHIONEIN)启动子和牛乳突瘤病毒在哺乳动物细胞中表达乙型肝炎表面抗原
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     On the Distribution of Acidic Proline-Rich Proteins (APRPs)Phenotypes in Chinese Han Population (Liaoning Area)
     中国(辽宁地区)汉族唾液酸性富含脯氨酸蛋白(APRPs)多型分布的研究
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     Quantitative Study on Ag-NORs in Nucleolar Organizer Regions in Gastric Cancer and Gastric Ulcer
     胃癌与胃溃疡病变的核仁组成区嗜银蛋白(Ag-NORs)定量研究
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     CLONING AND EXPRESSION OF THE P24 GENE OF HUMAN T-CELL LYMPHOTROPIC VIRUS TYPE-I(HTLV-I) IN ESCHERICHIA COLI
     人嗜T淋巴细胞白血病病毒I型(HTLV-Ⅰ)核心蛋白(p24)基因的克隆及在大肠杆菌中的表达
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     MLL protein;
     MLL蛋白;
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     proteinuria(-);
     尿蛋白(-);
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     RADIOIMMUNOASSAY OF F PROTEIN
     F蛋白的放射免疫分析
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     (4) other proteins.
     (4)其它蛋白.
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  protein
Polyamines May Modulate Both G Protein-Coupled Receptors and G Proteins
      
Heterodimerization of G-Protein-Coupled Receptors Reveals an Unexpected Level of Pharmacological Diversity
      
The Activation Mechanism of Class-III G-Protein Coupled Receptors
      
Hepatic glutathione, lipid peroxides, glutathione peroxidase, alcohol dehydrogenase, aldehyde dehydrogenase, glycogen and total protein in liver were also significantly altered.
      
3D QSAR STUDIES OF INHIBITORS OF CHOLESTEROL ESTER TRANSFER PROTEIN (CETP) BY CoMFA, CoMSIA AND GFA METHODOLOGIES
      
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Owing to the deficiency of lysine in zein, the hydrolysate of this protein was used as the main source of amino acids in the preparation of the medium for microbiological estimation of lysine using Lcuconostoc mcsentcroides P-60. as the test ing microorganisms. Suitable quantities of tryptophane, arginine, cystine, glycine and serine were used to supplement the medium.The maximum rate of acid production by the microorganism in this medium is much higher than that in the medium used by Horn et al for the same...

Owing to the deficiency of lysine in zein, the hydrolysate of this protein was used as the main source of amino acids in the preparation of the medium for microbiological estimation of lysine using Lcuconostoc mcsentcroides P-60. as the test ing microorganisms. Suitable quantities of tryptophane, arginine, cystine, glycine and serine were used to supplement the medium.The maximum rate of acid production by the microorganism in this medium is much higher than that in the medium used by Horn et al for the same purpose. The latter medium shows a rapid increase of acid production up to 80 μg lysine per innoculated test, while our new medium gives a continuous increase of acid production up to 120μg. Thus, the range in which the amino acid can be estimated in much extended.Parallel analysis was made on 25 food samples with our new medium as well as those published in the literature. In 11 cases, the agreement was within 10%; in 13 cases, the results obtained with' our new medium were about 10% higher; and in only 1 case, the new medium gave a lower result. These differences are not due to routine analytical error as repeated estimation gave similar results.

本文介绍微生物法测定赖氨酸所用的一种经济培养基。其大部分氨基酸系由玉米胶蛋白水解物供给。补充的氨基酸为精氨酸、甘氨酸、色氨酸、胱氨酸和丝氨酸。 实验结果证明,用玉米胶蛋白作培养基,以测定食物中赖氨酸的含量时,可获得较稳定的结果和满意的收回率。用本培养基测定一般食物中赖氨酸的含量与用其他培养基测定的结果符合,故本培养基可以用于食物中赖氨酸的测定。

(1)The present communication is a continuation of previous study of drugs on the pathologico-physiological reactions of the infected rabbits.The following indices of the reactions were used,namely:(1) erythrocyte sedimentation rate (ESR),(2) plasma fibrinogen content,(3) plasma gamma globulin content,(4) prothrombin time and (5)body weight. (2)It was shown that a full course of potassium antimony tartrate (PAT) could depress the abnormal rise of ESR of the infected rabbit.The effect was more striking in the...

(1)The present communication is a continuation of previous study of drugs on the pathologico-physiological reactions of the infected rabbits.The following indices of the reactions were used,namely:(1) erythrocyte sedimentation rate (ESR),(2) plasma fibrinogen content,(3) plasma gamma globulin content,(4) prothrombin time and (5)body weight. (2)It was shown that a full course of potassium antimony tartrate (PAT) could depress the abnormal rise of ESR of the infected rabbit.The effect was more striking in the early (i.e.7th day after inoculation) than the late treatment (i.e.33rd day after inoculation).This effect of PAT on the ESR was found to be in parallelism with its therapeutic effect.This method may be utilized for evaluation of drugs against schistosomiasis. (3)Neither PAT nor strychnine had any effect on the ESR of the normal rabbit.Strychnine was also found to have no significant effect on the ESR of the infected rabbit,but it could markedly modify the inhibitory effect of the PAT. (4)The therapeutic effect of half-course of PAT was found to be about 10—20% less marked than that of combined treatment with strychnine. Strychnine alone did not show any therapeutic effect.It was also found that the dose of strychnine used in our experiment did not increase the toxicity of PAT as shown by mice toxicity test. (5)The therapeutic dose of PAT showed no effect on the plasma fibrinogen content of the normal rabbit but it could bring the increased plasma fibrinogen content back to normal in the infected animal. (6)After a course of PAT in the infected animal,the double peak rise of the palsma gamma globulin content was distinctly suppressed as compared with the control group.On the contrary,the new drug,1:7-Bis (p-dimethylaminophenoxy) heptane (APH) did not show the same effect, probably indicating its weak action against schistosomiasis. (7)The body weight of the PAT treated group was found to be much higher than the non-treated group,However,the group treated with APH showed a continuous drop of body weight. (8)It has been found,as by others,that the APH showed high toxicity and low therapeutic effect in the experimental animals.From the fact that APH showed pronounced effect on ESR and plasma fibrinogen content of the infected animal,one could not yet decide,with the available data,whether these effects are due to its action on the schistosome or its eggs,or to its toxic action on the host.

(一)本文报告了药物对于血吸虫病病兔及正常家兔的红血球沉降率,血浆纤维蛋白,丙种球蛋白,凝血酶元时值及体重的影响。(二)酒石酸锑钾的治疗,无论在家兔患病的早期或后期,均可抑制其血沉的加速,而以早期治疗的抑制作用更为显著。同时这个抑制血沉的作用与治疗后的成虫发育率是符合的。因此利用这种方法,可以考虑作为研究一些治疗血吸虫病药物的疗效指标。(三)酒石酸锑钾及士的宁本身对正常家兔的血沉并不引起改变,士的宁对于病兔的血沉也无明显作用,但士的宁可以影响酒石酸锑钾对病兔血沉的抑制作用。 (四)半疗程剂量酒石酸锑钾并用士的宁之疗效较单独应用半疗程剂量酒石酸锑钾者为高(10—20%),而单独应用士的宁并无任何疗效,轻度激动量士的宁也不增加酒石酸锑钾的毒性。(五)酒石酸锑钾的治疗剂量对于正常家兔之血浆纤维蛋白元无明显影响,但能使病兔增高之血浆纤维蛋白元回复正常。(六)酒石酸锑钾的治疗可以使病兔的丙种球蛋白在一定时期内保持在接近正常值范围内,这一抑制作用与感染对照组相比,甚为明显,而氨苯氧烷却无此作用,这一点可能与疗效是有关系的。(七)酒石酸锑钾治疗后的病兔之体重较感染...

(一)本文报告了药物对于血吸虫病病兔及正常家兔的红血球沉降率,血浆纤维蛋白,丙种球蛋白,凝血酶元时值及体重的影响。(二)酒石酸锑钾的治疗,无论在家兔患病的早期或后期,均可抑制其血沉的加速,而以早期治疗的抑制作用更为显著。同时这个抑制血沉的作用与治疗后的成虫发育率是符合的。因此利用这种方法,可以考虑作为研究一些治疗血吸虫病药物的疗效指标。(三)酒石酸锑钾及士的宁本身对正常家兔的血沉并不引起改变,士的宁对于病兔的血沉也无明显作用,但士的宁可以影响酒石酸锑钾对病兔血沉的抑制作用。 (四)半疗程剂量酒石酸锑钾并用士的宁之疗效较单独应用半疗程剂量酒石酸锑钾者为高(10—20%),而单独应用士的宁并无任何疗效,轻度激动量士的宁也不增加酒石酸锑钾的毒性。(五)酒石酸锑钾的治疗剂量对于正常家兔之血浆纤维蛋白元无明显影响,但能使病兔增高之血浆纤维蛋白元回复正常。(六)酒石酸锑钾的治疗可以使病兔的丙种球蛋白在一定时期内保持在接近正常值范围内,这一抑制作用与感染对照组相比,甚为明显,而氨苯氧烷却无此作用,这一点可能与疗效是有关系的。(七)酒石酸锑钾治疗后的病兔之体重较感染对照组病兔显著增高,而经氨苯氧烷治疗后之病兔,其体重仍日见减轻。(八)氨苯氧烷有较高的毒性,但疗效甚低,它对病兔的血沉及纤维蛋白元所出现的抑制作用,究竟属于对血吸虫或其虫卵的作用,抑系对于病兔机体的毒性作用,目前还不能肯定。

Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter...

Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter with hydrogen sulphide,followed by paper electrophoresis and paper chromatography.The purified product has been separated into four flavin-adenine peptides with different amino acid contents.One fraction with comparatively high mobility on paper electrophoresis and containing 12 amino acids(hydrolyzed in 6 N HCl) has an absorption spectrum with maxima at 265,350 and 450 mμ(compared with 260,375 and 450 mμ of FAD),the ratio of E_(260) and E_(450) mμ is equal to 3.87.The other three fractions has similar absorption spectra as that of the first,except for a slight shift of the 265 mμ maximum to 270 mμ.All the four flavin-adenine peptides contain cysteine and show a greenish yellow fluorescence in the u.v.light.The fluorescent intensity of the prosthetic group varies with pH and exhibits a maximum at pH 2.9.All fractions are inactive in the D-amino acid oxidase test and give on analysis 1 mole of adenine and 2.5 moles of phosphorus per mole of flavin.The pentose flavin ratio was much higher than that of FAD. Photolysis of the flavin-adenine peptides in alkaline solution yields a product which is insoluble in chloroform after acidification.Removal of the adenine results in the formation of flavin peptides.These facts indicate that the peptide chain is linked to the isoalloxazine nucleus of the prosthetic group.It is known that the absorption peak at 375 mμ of either FAD or FMN shifts to about 355 mμ at pH 12 due probably to the enolization of the keto group at the 2 or 4 position resulting in a redistribution of double bonds in the isoalloxazine ring system. In contrast our flavin-adenine peptide has the corresponding absorption maximum at 350 mμ which shows little positional shift at pH 12.This seems to suggest that the linking of the peptide chain to the isoalloxazine nucleus affects the enolization of the-NH-CO-,which may probably be the site of the linkage. The iron content increases with the specific activity of the enzyme during purification.Iron in the purified enzyme is present in the reduced state.It is firmly bound to the enzyme and can not be removed by prolonged dialysis against phosphate buffer or tris-hydroxymethyl-amino-methane buffer.Enzymatic activity is lost during prolonged incubation with o-phenanthroline or α,α'- dipyridyl and can not be recovered by incubation with Fe~(2+) or Fe~(3+).These experiments de- monstrate a close relationship between enzyme activity and the iron present in the enzyme molecule. The enzyme activity is lower in borate than in phosphate buffer.When 40 mM ethylenedi- aminetetraacetic acid is added to the borate buffer,the enzyme activity is raised almost to the level of that observed in the same concentration of phosphate buffer.The effect of EDTA and phosphate,when present together,is somewhat higher than of either alone.Alanine has a similar effect'as EDTA.

(一)用结晶胰蛋白酶及结晶胰凝乳蛋白酶处理净化的水溶性琥珀酸脱氢酶,经过对甲酚抽提,硫酸汞沉淀,硫化氢分解及纸电泳纸层析等方法净化得到四种带有不同肽链的腺嘌呤异咯嗪核苷酸。从它们的组成成份的分析以及它们的性质的观察,我们认为它们与已知的腺嘌呤异咯嗪二核苷酸略有不同。肽链是连接在异咯嗪上,其连接方式异于一般异咯嗪蛋白。肽链部份的氨基酸组成的分析结果,证明它们都含有半胱氨酸。(二)琥珀酸脱氢酶中的铁处于还原状态。铁与酶朊紧密结合,它与酶活力有密切关系。(三)无机磷可增加琥珀酸脱氢酶的活力,但琥珀酸脱氰酶的活力并不是必需依靠无机磷的存在,乙二胺四乙酸与丙氨酸也有类似无机磷的作用。

 
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