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csf重组
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  csf recombinant
     Murine dendritic cells were generated from bone marrow by GM CSF recombinant adenoviral infection,and their biological activities studied.
     用GM-CSF重组腺病毒感染小鼠骨髓细胞,观察扩增到的骨髓树突状细胞的生物学功能。
短句来源
  “csf重组”译为未确定词的双语例句
     Study of the stability of recombinant human G-CSF expression system
     人G-CSF重组表达系统稳定性的实验研究
短句来源
     Objective To construct recombiant plasmid pc-mGM-CSF and lay a primary foundation for further study of mGM-CSF gene therapy for tumors.
     目的 构建pc mGM CSF重组质粒载体 ,为mGM CSF基因治疗肿瘤的研究奠定基础。
短句来源
     Construction,Expression and its Activity Examinations of Recombiant Plasmid with mGM-CSF
     mGM-CSF重组质粒的构建、表达及活性鉴定
短句来源
     Methods: Recombinant plasmids pIRES-PSMA-mGM-CSF,pIRES-PSMA,and pIRES-mGM-CSF were constructed with DNA vaccine vector pIRES. After identified by endonuclease digestion,the above 3 plasmids and blank pIRES vector were used to immunize C56BL/6 mice(n=15). LDH release assay was used to exam the cytotoxicity of cytolytic T lymphocytes in each group.
     方法:应用DNA疫苗载体pIRES构建pIRES-PSMA-mGM-CSF、pIRES-PSMA、pIRES-mGM-CSF重组质粒,酶切鉴定正确后与pIRES空质粒分别免疫C56BL/6小鼠(每组15只),LDH释放试验测定各自免疫后小鼠的特异性细胞毒性T细胞(CTL)杀伤活性。
短句来源
     Construction and Identification of Recombinant Adenovirus Vector Containing rhGM-CSF Gene
     rhGM-CSF重组腺病毒载体的构建及鉴定
短句来源
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  相似匹配句对
     The recombinant plasmid expressed well in E.
     从重组E.
短句来源
     Overproduction of Recombinant hG CSF With High Activity
     高活性重组hG-CSF的制备
短句来源
     Construction and Identification of Recombinant Adenovirus Vector Containing rhGM-CSF Gene
     rhGM-CSF重组腺病毒载体的构建及鉴定
短句来源
     Reform of the Order
     秩序的重组
短句来源
     (2)The contrast sensitivity function decline with the age.
     (2)CSF
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  csf recombinant
Using a newborn piglet model of acute lung injury, we examined the effects of rhG-CSF (recombinant-metHuG-CSF) on lung injury.
      


In recent years, the evaluation of growth factors (GFs) in the regulation of hemopoiesis in the marrow of patients with hematological clonal diseases are developed rapidly. We used semi-solid culture in vitro to study the effect of human recombinant GFs, rhG-CSF, rhEPO and PHA-MNCCM on marrow cells in fifteen patients with AML, four patients with CML and seven patients with MDS.

近年,有关造血因子对造血调控的研究比较活跃。本文应用体外半固体培养,就重组人粒细胞集落刺激因子(rhG-CSF)、重组人红细胞生成素(rhEPO)及PHA-MNCCM对15例初诊急性粒细胞白血病(AML)、4例慢性粒细胞白血病(CML)和7例骨髓增生异常综合征(MDS)患者骨髓细胞的体外增殖作用以及刺激因子间联合作用进行了观察。 实验结果显示AML细胞对各种刺激因子的反应均不完全相同,表现在集落、丛的数量和大小的变化。rhG-CSF、PHA-MNCCM均有刺激白血病细胞增殖与诱导分化作用。MDS骨髓细胞对造血因子的反应与白血病细胞有所不同,表现在对各种造血因子反应程度较一致。且3种生长因子同时加入有协同作用。对PHA有依赖性,但不如白血病细胞敏感。另外,观察到rhG-CSF对正常人CFU-GM、BFU-E的增殖分化有较强的刺激作用。

Effects of various hematopoietic growth factors including recombinant mouse interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), and recombinant human interleukin-6 (IL-6) and erythropoietin (EPO) on the growth of murine CFU-MK were studied using the plasma clot culture system. IL-3, GM-CSF, IL-6 and EPO each was able to stimulate the formation of megakaryocyte colonies in cultures. The concentrations inducing an optimal growth of CFU-MK are 100 U/ml for IL-3, 5 ng/ml for GM-CSF,...

Effects of various hematopoietic growth factors including recombinant mouse interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), and recombinant human interleukin-6 (IL-6) and erythropoietin (EPO) on the growth of murine CFU-MK were studied using the plasma clot culture system. IL-3, GM-CSF, IL-6 and EPO each was able to stimulate the formation of megakaryocyte colonies in cultures. The concentrations inducing an optimal growth of CFU-MK are 100 U/ml for IL-3, 5 ng/ml for GM-CSF, 20 ng/ml for IL-6 and 1 U/ml for EPO, respectively. These results indicate that IL-3, GM-CSF, IL-6 and EPO regulate positively megakaryocytopoiesis in vitro.

小鼠体外巨核系祖细胞培养表明,重组鼠白细胞介素-3(IL-3),粒-巨噬细胞集落刺激因子(GM-CSF)、重组人白细胞介素-6(IL-6)及红细胞生成素(EPO)均有不同程度刺激巨核系祖细胞生长的活性。其最适浓度分别为100U/ml,5ng/ml,20ng/ml与1U/ml。对巨核细胞集落刺激作用最强的是IL-3,IL-6与GM-CSF次之,EPO最弱。在种植2×10~5骨髓单个核细胞中分别获得45±3,25±2,20±3及10±2个巨核细胞集落。

recombinant transfer vector pAc610-GMTcarrying

本工作构建的昆虫表达重组体pAC610-GMT,是在AcMNPV的Polyhedrin启动子控制下,表达去除了信号肽编码顺序的人GM-CSF基因(cDNA)的转染载体。它与野生AcMNPV病毒DNA共转染Sf21细胞,经过筛选得到纯化的可表达人GM-CSF的重组病毒株vAcGMT。其感染细胞总RNA的Northern分析结果表明,重组病毒在mRNA水平有人GM-CSP特异性表达,其表达水平在感染后48h时达高峰,72h未见明显下降。感染细胞裂解物的Western-Blot分析和活性测定也证实其蛋白水平的表达,并有人GM-CSF的生物学活性。

 
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