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型前胶原
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     (2)C shape;
     C ;
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     (3)D shape.
     D
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     Isolation and purification of goat procollagen type Ⅲ
     羊Ⅲ前胶原的分离纯化
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     And the type I procollagen mRNA of keloid derived fibroblasts decreased significantly.
     其相应I前胶原mRNA量显著下降。
短句来源
     The AP-1 DNA binding protein increased after the treatment of AngⅡ and peaked 30 min after.
     Ang和Aldo可诱导α1Ⅰ前胶原mRNA的表达。
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A method for extraction of type Ⅲ procollagen from new born calf skin has been developed. Collagen in the skin were first extracted with neutral buffer solution in the presence of protease inhibitors. Type Ⅲ collagen in the extract were separated from other proteins by salt precipition, and the procollagen was further separated from the collagen by DEAE-cellulose chromatography.Antisera against the isolated type n procollagen were prepared in rabbit. A titer value of 1:100,000 was obtained by ELISA. Identification...

A method for extraction of type Ⅲ procollagen from new born calf skin has been developed. Collagen in the skin were first extracted with neutral buffer solution in the presence of protease inhibitors. Type Ⅲ collagen in the extract were separated from other proteins by salt precipition, and the procollagen was further separated from the collagen by DEAE-cellulose chromatography.Antisera against the isolated type n procollagen were prepared in rabbit. A titer value of 1:100,000 was obtained by ELISA. Identification by compare with the standard sample in ELISA kit for type 33333 procollagen from Forschanges Lab. as reference showed that our preparation was type Ⅲ procollagen.

在中性pH和蛋白酶抑制剂的存在下,从新生小牛皮肤中提取Ⅲ型前胶原。利用反复盐析法将Ⅰ型胶原和前胶原与Ⅲ型胶原和前胶原分开,再经DEAE纤维素层析分离出Ⅲ型前胶原。 用提取的Ⅲ型前胶原免疫家兔,获得抗血清,用酶联免疫法测定效价达1:10O,000。经与国外标准品鉴定该提取物为Ⅲ型前胶原

In this report procollagen type I (PCI) was isolated and purified from human fetal skin of induced abortion. The characteristic of this antigen was successfully identified. There was only one band on polyacrylamide gel electrophoresis; Two bands namely Pro a1 (III) and Pa1(III) were presentedafter reduction, the molecular weight of former was 190 000 and later 170 000; The high value of antiserum was aquired from rabbit after immunization; Collagen fibers of biopsies with cirrhosis and chronic active hepatitis...

In this report procollagen type I (PCI) was isolated and purified from human fetal skin of induced abortion. The characteristic of this antigen was successfully identified. There was only one band on polyacrylamide gel electrophoresis; Two bands namely Pro a1 (III) and Pa1(III) were presentedafter reduction, the molecular weight of former was 190 000 and later 170 000; The high value of antiserum was aquired from rabbit after immunization; Collagen fibers of biopsies with cirrhosis and chronic active hepatitis were stained with brown colour on immunohistochemisty. So far there is no report about the isolation of PCII from human source.

本文从雷佛诺尔引产的胎儿皮肤提取、纯化Ⅱ型前胶原,经聚丙酰胺凝胶电泳证实为单一区带;经还原后得到两个亚单位:Proα_1(Ⅱ)与pα_1(Ⅱ),前者分子量为19万,后者为17万。用此抗原免疫家兔获得高效价抗血清。免疫组化可见肝硬化与慢活肝的肝组织中的胶原染为棕色。本文系首次报道从人工流产的人胎皮中提纯Ⅱ型前胶原获得成功。

The present study is concerned with the fibronectin(Fn) distribution,the formation character and its source in atherosclerotic lesi- ons using immunohistochmical and immunoelectronic microscopy.The resu- lts showed that large amount of Fn was localized in fatty streaks,gelati- nous lesions and early atherosclerotic plaques.The smooth muscle cells of intima synthesize more Fn in atherosclerotic lesions.It is likely that Fn represents a new marker of smooth muscle cell modulating from “contrac- tile”to“synthetic”state.With...

The present study is concerned with the fibronectin(Fn) distribution,the formation character and its source in atherosclerotic lesi- ons using immunohistochmical and immunoelectronic microscopy.The resu- lts showed that large amount of Fn was localized in fatty streaks,gelati- nous lesions and early atherosclerotic plaques.The smooth muscle cells of intima synthesize more Fn in atherosclerotic lesions.It is likely that Fn represents a new marker of smooth muscle cell modulating from “contrac- tile”to“synthetic”state.With the maturation of atherosclerotic plaque, Fn does not fill the whole plaque but is concentrated only in the fibrous cap surface and basocentral atheroma.We also prove that proeollagen Ⅲ peptide(PⅢP)distribution in atherosclerotic plaque is similar to Fn.

应用免疫组织化学和免疫电镜技术,研究人体动脉粥样硬化病灶纤维连接蛋白的分布、形态特征和来源,结果发现在脂纹、胶样病变及早期斑块的内膜上有大量纤维连接蛋白沉积。纤维连接蛋白主要由内膜平滑肌细胞合成,平滑肌细胞表达纤维连接蛋白的能力也可能是反映其表型改变的一个标志。随着斑块的成熟、纤维化,纤维连接蛋白则减少。动脉粥样硬化斑块Ⅲ型前胶原肽的分布与纤维连接蛋白具有一致性。

 
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