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   膀胱癌细胞系 的翻译结果: 查询用时:0.073秒
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膀胱癌细胞系     
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  bladder cancer cell line
     Expression and identify of CH50 polypeptide in bladder cancer cell line BIU-87
     CH50多肽在膀胱癌细胞系BIU-87中的表达和鉴定
短句来源
     A human RT4 bladder cancer cell line stably expressing green fluorescent protein(GFP)-PKCα (RT4/PKCα) was established.
     应用人浅表膀胱癌细胞系RT4细胞,建立能够稳定表达PKCα绿色荧光蛋白(GFP)的人膀胱癌细胞系RT4/PKCαGFP。
短句来源
     Effect of transfection of pCH510 into bladder cancer cell line BIU-87 on the antitumor function of BCG
     pCH510转染膀胱癌细胞系BIU-87对卡介苗抑瘤作用的影响
短句来源
     Effects of PTEN Gene Transfection on Proliferation and Invasion of Human Bladder Cancer Cell Line BIU87
     PTEN基因转染对人膀胱癌细胞系BIU87增殖及侵袭活性的影响
短句来源
     Expression of MTS1 in Human Bladder Cancer and Inhibitory Effects of Wild-type MTS1 on Human Bladder Cancer Cell Line
     MTS1在膀胱癌中的表达及野生型MTS1对人膀胱癌细胞系生长抑制作用的研究
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  bladder cancer cell lines
     Objective: To evaluate the effect of exogenous human tumor suppressor gene nm23-H1 on the chemotherapeutic sensitivity of the bladder cancer cell lines T-24 cell.
     目的: 探讨外源性人抑癌基因nm23 H1 对膀胱癌细胞系T -24 细胞化疗敏感性的影响。
短句来源
     Methods The bladder cancer cell lines 5637 and EJ were treated with 3 types of drug combinations,ie,GEM plus TAX simultaneously (group A),GEM 24 h ahead of TAX (group B) and TAX 24 h ahead of GEM (group C).
     方法MTT法检测GEM与TAX同时(A组)、先后间隔24h时序(B组为GEM先于TAX24h,C组为TAX先于GEM24h)联合用药对膀胱癌细胞系5637和EJ细胞的细胞毒活性,Chou和Talalay方法评估两药物作用关系;
短句来源
     In order to evaluate the correlation between the status of GJIC and the proliferation of tumor cells, two human bladder cancer cell lines, BIU and EJ and one ovarian cancer cell line, CaOvS were treated with GJIC enhancer dB-cAMP, anticancer agent cisplatin(DDP) and dB-cAMP+DDP,respectively.
     为探讨肿瘤细胞缝隙连接通讯(GJIC)的功能状态对化疗药物抗癌效果的影响,本研究选用GJIC功能迥异的人膀胱癌细胞系BIU,EJ和卵巢癌细胞系CaOv3,借助多种细胞生物学手段,观察它们对广谱抗癌药顺铂(DDP)和GJIC调节剂dB-cAMP的反应。
短句来源
     Objective:To investigate the inhibitory effects of STAT3 decoy ODNs on the growth of human bladder cancer cell lines in vitro.
     目的:研究核转录因子STAT3的诱骗寡核苷酸(decoy ODNs)对人膀胱癌细胞系增殖的抑制作用,并探讨其作用机制。
短句来源
     Study on the efficacy of scheduled combination of gemcitabine with paclitaxel against human bladder cancer cell lines
     吉西他滨、紫杉醇时序联合对膀胱癌细胞系细胞毒效应研究
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  human bladder cancer cell line
     Effects of PTEN Gene Transfection on Proliferation and Invasion of Human Bladder Cancer Cell Line BIU87
     PTEN基因转染对人膀胱癌细胞系BIU87增殖及侵袭活性的影响
短句来源
     Expression of MTS1 in Human Bladder Cancer and Inhibitory Effects of Wild-type MTS1 on Human Bladder Cancer Cell Line
     MTS1在膀胱癌中的表达及野生型MTS1对人膀胱癌细胞系生长抑制作用的研究
短句来源
     Objective To investigate the role of protein kinase C- alpha (PKCα) in regulating multidrug resistance (MDR) in human bladder cancer cell line T24.Methods Plasmid green fluorescent protein -PKCα (GFP/PKCα) carrying PKCα gene was transfected into a human T24 bladder cancer cell line.
     目的探讨蛋白激酶C-α(PKCα)对人膀胱癌T24细胞多药耐药的调节作用。 方法将载有PKCα基因的表达质粒GFP-PKCα导入人膀胱癌细胞系T24。
短句来源
     To study the resistance mechanism of human bladder cancer cell line, an adriamycin(ADM) resistance bladder cancer cell line, BIU 87/A and an etoposide (VP 16) resistance cell line, BIU 87/V were established by exposing the BIU 87 parent cells to a culture medium with a persistent increase of concentration of ADM or VP 16 respectively over a period of 10 months.
     为研究人膀胱癌细胞株耐药机制,应用阿霉素(ADM)、足叶乙甙(VP-16)低浓度持续递增法,在体外持续培养10个月,逐步诱导人膀胱癌细胞系BIU-87,获得具有多重抗药性的BIU-87/A、BIU-87/V细胞。
短句来源
     Cyclooxygenase Inhibitor Suppressed COX-2 Expression and Induced Apoptosis in Human Bladder Cancer Cell Line T24
     环氧化酶抑制剂诱导人膀胱癌细胞系T24凋亡作用研究
短句来源
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  bladder tumor cell line
     Inhibition of human bladder tumor cell line EJ by adenovirus mediated over expression of human p27 KIP1
     Adp27~(KIP1)基因的过度表达对人膀胱癌细胞系EJ的抑制作用
短句来源
     The expression of mRNA by RT PCR of 5 human mismatch repair(MMR) genes in bladder tumor cell line was evaluated.
     用RT PCR方法监测 5种人类错配修复基因在膀胱癌细胞系mRNA转录水平的表达 ;
短句来源
     Objective To investigate the expression and identification of CH50 polypeptide in bladder tumor cell line BIU-87 by gene transfection of pCH510,and study the effect on the antitumor function of BCG to BIU-87.Methods The eukaryotic expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfection Lipofectimine TM 2 000,and the expressed product was identified by immunohistochemistry S-P method and Western blot method.
     目的研究膀胱癌细胞系BIU-87体外转染pCH510后,CH50多肽的表达及其对卡介苗(bacilluscalmette-guerin,BCG)抑瘤作用的影响。 方法在LipofectimineTM2000的介导下,将质粒pCH510体外转染给BIU-87细胞,采用免疫组织化学S-P法和WesternBlot法鉴定CH50多肽的表达;
短句来源
     Human bladder tumor cell line (BT5637) was managed by HESW(high energy shock waves,electrohydrulically generated, ESSL-Ⅲ/2M type) alone or in combination with 10-hydroxy camptothecimun(an anti-tumor drug).
     利用国产液电碎石机(ESWL-Ⅲ/2M型)产生的高能冲击波和10-羟基喜树碱单纯或联合处理人膀胱癌细胞系(BT5637)。
短句来源

 

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  bladder cancer cell line
Preliminary study of the in vitro growth inhibition of human bladder cancer cell line BIU-87 by arsenic trioxide
      
The growth inhibition rates of human bladder cancer cell line BIU87 by various concentrations of As2O3 were detected by using MTT method.
      
Under the induction of Lipofectamine 2000, the recombinant was transfected into Uroplakin II negative bladder cancer cell line EJ.
      
Effects of gene tranfection with CH50 polypeptide on the invasion ability of bladder cancer cell line BIU-87
      
The expression of CH50 polypoptide in bladder cancer cell line BIU-87 and the effects on the invasion ability of BIU-87 were investigated.
      
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  bladder cancer cell lines
Implantation of human bladder cancer cell lines in the bladder wall of nude rats results in tumor formation, providing an excellent model to test this.
      
Effector cells in natural cytotoxicity against human bladder cancer cell lines
      
Hu-IFN-gamma was evaluated in regard to the antiproliferative effect on J82 and 647V bladder cancer cell lines.
      
Marked glycosylation of sublines of both bladder cancer cell lines was found compared with normal human bladder transitional epithelium (assessed cytochemically).
      
Previously we have shown a differential biological response of three human bladder cancer cell lines (RT4, RT112 and MGH-U1) to gamma interferon (IFN-γ).
      
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  human bladder cancer cell line
Preliminary study of the in vitro growth inhibition of human bladder cancer cell line BIU-87 by arsenic trioxide
      
The growth inhibition rates of human bladder cancer cell line BIU87 by various concentrations of As2O3 were detected by using MTT method.
      
Comparison of the cytotoxic activities of chemotherapeutic drugs using a human bladder cancer cell line
      
A human bladder cancer cell line was exposed to a range of concentrations of the four drugs commonly used to treat superficial bladder cancer (adriamycin, epodyl, mitomycin-c, thiotepa) for periods of 30, 60 and 120 min.
      
Drug sensitivity of the human bladder cancer cell line J-82 was assessed using monolayer, stem cell and [3H]thymidine incorporation assays.
      
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  bladder tumor cell line
A rat bladder tumor cell line, NBT-II, expressed both TGF-α and c-met mRNAs, and HGF showed apparent scattering and growth-stimulating effects on the cells.
      
This study was designed to investigate the relationship between expression of matrix metalloproteinase-2 (MMP-2) and invasiveness of human bladder cancer cells, using cell lines derived from a parental human urinary bladder tumor cell line, T24.
      
Our previous studies demonstrated that human bladder tumor cell line (T24) has N-acetyltransferase (NAT) activity in cytosols and intact cells.
      
The aim of the present study was to determine whether sulindac affects arylamine N-acetyltransferase (NAT) activity and gene expression and DNA-2-aminofluorene adduct formation in the T24 human bladder tumor cell line.
      
Human lymphocytes and cells from the T24 bladder tumor cell line were used as controls.
      
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  其他


A newly established cell line, derived from human bladder carcinoma, was designated as BIU-87.Its chromosome G-banding karyotype and clinical implication were studed. The chromosome number ofthe BIU-87 was characterized as hetertetraploid. By analysing 100 mitosis cells, the modal count was101 and the structure was quite diffrent from that of normal cell. A total of 12 marker chromosomeswas discovered. Notably. the M 1 and M 2 marker chromosomes are related to No. 1 chromosome. Weconclude that it could be of...

A newly established cell line, derived from human bladder carcinoma, was designated as BIU-87.Its chromosome G-banding karyotype and clinical implication were studed. The chromosome number ofthe BIU-87 was characterized as hetertetraploid. By analysing 100 mitosis cells, the modal count was101 and the structure was quite diffrent from that of normal cell. A total of 12 marker chromosomeswas discovered. Notably. the M 1 and M 2 marker chromosomes are related to No. 1 chromosome. Weconclude that it could be of help to dignosis of bladder carcinoma. The presence of some doubleminute (DM) may imply the relationship between malignant cell and the oncogene.

新建的人膀胱癌细胞系命名为BIU-87,本文研究其染色体及其G带的变化及临床意义。BIU-87细胞有明显的超4倍体特点,分析100个中期细胞得出染色体众数平均为101条。染色体形态结构与正常细胞相比有很大不同,可检出12个异常的标记染色体。其中最为明显的M_1和M_2标记染色体都与1号染色体有关。这将有助于临床辅助诊断膀胱癌。双小体的存在提示了细胞癌变和癌基因变化之间的关系。

Clinical and experimental researches on biologic behayior of bladder cancer in our Institute in recentyears have been summarized. Clinical studies revealed the influence of mucosal lesions accompaniedwith bladder cancer on the occurrence and progress of the tumor, short and long term herapeutic effectof intravesical BCG instillation for bladder cancer. Experimental reseaches included the establishmentof epithelial cell line BIU-87 from a human bladder transitional cell carcinoma, the production of amonoclonal...

Clinical and experimental researches on biologic behayior of bladder cancer in our Institute in recentyears have been summarized. Clinical studies revealed the influence of mucosal lesions accompaniedwith bladder cancer on the occurrence and progress of the tumor, short and long term herapeutic effectof intravesical BCG instillation for bladder cancer. Experimental reseaches included the establishmentof epithelial cell line BIU-87 from a human bladder transitional cell carcinoma, the production of amonoclonal antibody (BDI-1) against human bladder carcinoma. radioimmunoimaging of the human blad-der cancer xenografts by ~(3)I labeled monoclonal antibody BDI 1, specific killing effect of monoclonalantibody BDI-1 conjugated adriamycin on bladder tumor, and the expression of oncogenes of human blad-der cancer (Southern blot and Northern blot).

本文总结了近年来我所有关膀胱癌生物学特性的临床及实验研究。临床方面研究了膀胱癌伴随的粘膜病变对肿瘤发生发展的影响,并对BCG治疗膀胱癌的近远期疗效作了观察。实验研究主要是建立了膀胱癌细胞系(BIU-87),制备了膀胱癌单克隆抗体,应用单抗对载瘤裸鼠进行了放射免疫肿瘤显像,在体内外显示了偶联上阿霉素的单抗对膀胱癌细胞的特异杀伤作用,对膀胱癌基因的表达进行了研究。

Based on the high level of human granulocyte—macrophage colony—stimulating factor(HGM—CSF)re-

本文应用人髓样白血病细胞系 HL60在了 DMSO 诱导下可表达高亲和力 HGM—CSF 受体的特点,建立了 HGM—CSF 生物学检测方法。结果表明:未经诱导的 HL60细胞对 HGM—CSF 只有很低的反应性,经 DMSO 诱导的 HL60细胞对 HGM—CSF 反应性明显增加,最适诱导时间为3~5天,检测时间为36小时,细胞密度以3~6×10~4/孔为宜,检测敏感性至少可达0.3ng/ml。TPA 和细胞因子 IL—1、IL—6、IL—8.均不能促进 DMSO 诱导的 HL60细胞对 HGM—CSF 的反应性。本文还应用此检则方法对人膀胱癌细胞系U5637培养上清中 GM—CSF 的生物学活性进行了检测。

 
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