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半酶
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  half enzymatic
     Inquiring into the new technology of half enzymatic hydrolysis half koji process to produce coy sauce
     半酶半曲法酶解酿造酱油新工艺的探讨
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  “半酶”译为未确定词的双语例句
     The synthetic DNA encoding for N-terminal dominant epitopes of core region of HCV polyprotein was synthesized with a semi-chemical and semi-enzymatic method and identified through DNA sequencing.
     依据丙型肝炎病毒(HCV)多蛋白核心区N端氨基酸序列和密码子简并性,人为设计并采用半化学半酶促的方法合成了一个DNA片段。 经核酸杂交检测以及DNA序列分析证实,该片段的核苷酸序列与设计完全一致。
短句来源
     Objective To construct the m odified gene of a cell grow th factor by the m ethods of P C R basedchem ical gene synthesis.
     目的 利用 P C R 介导的半酶促半化学法人工合成经改造的广谱细胞生长因子基因。
短句来源
     coli and investigate the effect of the recombinant protein on rat.Methold:The monomer sCT gene obtained by the chemical and enzyme methold was fused into the E. coli high efficient expression pTrcHisC vector resulting in pHis-CT from which a series of tandem repeated sCT gene was constructed.
     方法 :采用半化学半酶促法合成sCT基因 ,利用基因的特点及特殊的酶切位点NdeI和SamI在表达载体pTrcHisC中进行sCT基因与载体基因的融合及sCT基因的串联 ,并在TOP10中表达串联多拷贝基因。
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  相似匹配句对
     Generation of GPx Mimics Employed Semisynthetic Methods
     合成法构建GPx模拟
短句来源
     Using a new approach to engineer enzyme activity——semi-rational design
     活性设计的新方法——理性设计
短句来源
     Enzyme mimics
     模型
短句来源
     It couldn’t express in T.
     但该在T .
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     Half Beijinger
     个北京人
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  half enzymatic
IC50 is the concentration that results in half enzymatic activity with respect to untreated controls.
      


Human αcalcitonin gene-related peptide (hαCGRP) gene was synthesized by the combination of chemical synthesis and polymerase extenaion.The synthetic ha CGRP gene was fused to the 3' termini of the tumor negrosis factor-α(TNF-α)gene to form a TNF-CGRP fused gene and put directly under the control of the PL promoter of bacteriophage lambda. This fused gene could be expressed in E. coli with a level about 20% of total cellular Protein content.The expressed fuaion protein not only has ha CGRP immunological competence...

Human αcalcitonin gene-related peptide (hαCGRP) gene was synthesized by the combination of chemical synthesis and polymerase extenaion.The synthetic ha CGRP gene was fused to the 3' termini of the tumor negrosis factor-α(TNF-α)gene to form a TNF-CGRP fused gene and put directly under the control of the PL promoter of bacteriophage lambda. This fused gene could be expressed in E. coli with a level about 20% of total cellular Protein content.The expressed fuaion protein not only has ha CGRP immunological competence but also has TNF-αbiological activity.A major band of about 22kd could be seen by SDS-PAGE.The fusion protein was cleaved by cyanogen bromide and CGRP was found to be accurately fused to TNt-α.

半酶和半化学合成的方法合成和克隆了人α降钙素基因相关肽(CGRP)基因,将该基因融合在肿瘤坏死因子(TNF)之后插入表达载体pSB-92中,使融合基因的5’端直接置于大肠杆菌PL启动子下游,采用30℃培养,42℃诱导,使TNF与CGRP融合蛋白在大肠杆菌中获得了表达,表达的融合蛋白既有CGRP的结合活性(酶标检测为阳性〕又有TNF的生理功能(对L-929细胞的细胞毒活性)。表达菌裂解液走SDS-聚丙烯酰胺凝胶电泳,显示有1条约为22kd的蛋白质带。融合蛋白用CNBr裂解证明CGRP正确融合在TNF之后。

Objective To synthesize and express thymosin α1 (Tα1) gene in prokaryocyte.Methods Tα1 gene was synthesized and clone by zymological and chemical methods and inserted into expression plasmid pGEX 4T 1,then transformed to E.coli .The expressed fusion protein GST Tα1 was purified by affinity chromatography,digested with thrombin,and detected for biological activity.Results The DNA sequence of the synthesized Tα1 gene was identical to that designed.The constructed recombinant plasmid pGEX 4T 1 Tα1...

Objective To synthesize and express thymosin α1 (Tα1) gene in prokaryocyte.Methods Tα1 gene was synthesized and clone by zymological and chemical methods and inserted into expression plasmid pGEX 4T 1,then transformed to E.coli .The expressed fusion protein GST Tα1 was purified by affinity chromatography,digested with thrombin,and detected for biological activity.Results The DNA sequence of the synthesized Tα1 gene was identical to that designed.The constructed recombinant plasmid pGEX 4T 1 Tα1 was highly expressed in E.coli .E rosette test showed biological activity of the recombinant Tα1.Conclusion The synthesized Tα1 gene was successfully expressed in E.coli ,and the expressed protein showed biological activity.

目的 合成胸腺素α1(Tα1)基因并在原核细胞中表达。方法 用半酶半化学合成并克隆Tα1基因 ,将该基因插入表达质粒pGEX 4T 1并在大肠杆菌进行表达 ,对表达的融合蛋白GST Tα1利用亲和层析法纯化 ,再经Thrombin酶切后 ,获得重组Tα1进行生物学活性检测。结果 经测序证实合成的Tα1序列与设计的一致。构建的重组表达载体pGEX 4T 1 Tα1在大肠杆菌中得到高效表达。E玫瑰花结形成试验证明重组Tα1具有生物学活性。结论 合成的Tα1基因在大肠杆菌中表达出具有生物活性的蛋白。

 
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