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   肌动蛋白基因启动子 的翻译结果: 查询用时:0.013秒
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肌动蛋白基因启动子
相关语句
  actin gene promoter
     Cloning of Bombyx mori Cytoplasimic Actin Gene Promoter and Construction of piggyBac Transposon Expression Vector
     家蚕胞质肌动蛋白基因启动子的克隆及piggyBac转座子表达载体的构建
短句来源
     Cloning and sequence analysis of actin gene promoter in Haliotis discus hannai
     皱纹盘鲍肌动蛋白基因启动子的克隆和序列分析
短句来源
     To raise carp's resistance to hemorrhage,a large amount of transgenic grass carp was obtained by transfering hu IFN α gene which was cloned under the control of the β actin gene promoter of grass carp to the cytula of carp in the way of microinjection.
     为提高草鱼对出血病的抗性 ,采用显微注射法 ,将克隆在鲤 β-肌动蛋白基因启动子下游的人 α-干扰素基因转移到草鱼受精卵中 ,获得了大量转基因个体 .
短句来源
     The coding sequence of hu IFN α gene is cloned under the control of Carp β actin gene promoter. Thus a fish constantly expression of hu IFN α gene recombinant is constructed.
     通过分子重组 ,将人α 干扰素 (hu IFN α)基因编码序列克隆到鲤鱼β 肌动蛋白基因启动子下游 ,构建成能在鱼体内组成型表达hu IFN α的基因重组分子。
短句来源
  “肌动蛋白基因启动子”译为未确定词的双语例句
     Cloning of a Cytosol-localized hsp70 DNA from Dunaliella Salina and the Actin Gene Promoter-driven Bar as a Dominant Selectable Marker for Nuclear Transformation of D.salina
     杜氏盐藻hsp70基因的克隆及其肌动蛋白基因启动子驱动bar基因作为转基因筛选标记
短句来源
     β-actin is a housekeeper gene, and has a potent promoter which can improve transgene expression level efficiently without the limitation of tissue.
     由管家基因的身份所决定,β-肌动蛋白基因的启动子为强启动子,对于提高转基因表达量具有显著效果,且其表达不受组织的限制,这两大优点使得β-肌动蛋白基因启动子在转基因动物的研究中发挥着重要的作用。
短句来源
     The Actin Gene Promoter-driven bar as a Dominant Selectable Marker for Nuclear Transformation of Dunaliella salina
     盐藻肌动蛋白基因启动子驱动的bar基因表达作为核转化筛选标记(英文)
短句来源
     In 1998, 1999, by adopting the technology of molecule recombination, our subject team successfully cloned the coding sequence of HU-a-IFN gene under the control carp β -actin gene promoter. To build up the HU-a -IFN recombinat gene which is able to expressed in the fish body.
     1998、1999年以章怀云教授带领的课题组通过分子重组,将人-α-干扰素(Hu-IFN-α)基因编码序列克隆到鲤鱼β-肌动蛋白基因启动子下游,构建了能够在鱼体内组成型表达的Hu-IFN-α基因重组分子。
短句来源
     The result shows after the sequence is analysed: The homologous series of nuleotides sequence between Haliotis discus hannai and Haliotis rufescens is 95%.
     分析测序结果发现,皱纹盘鲍 肌动蛋白基因启动子DNA序列与目前已知的红鲍相应序列的相似度为95%;
短句来源
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  相似匹配句对
     Cloning and sequence analysis of actin gene promoter in Haliotis discus hannai
     皱纹盘鲍肌动蛋白基因启动子的克隆和序列分析
短句来源
     Analysis of genomic structure and promoter characterization of mud carp (Cirrhinus molitorella) β-actin gene
     鲮β-肌动蛋白基因启动子的克隆及序列特征分析
短句来源
     4 new genes were obtained.
     U基因
短句来源
     Cloning of Bombyx mori Cytoplasimic Actin Gene Promoter and Construction of piggyBac Transposon Expression Vector
     家蚕胞质肌动蛋白基因启动子的克隆及piggyBac转座子表达载体的构建
短句来源
     Cloning of Medicinal Gene and Promoter from Ganoderma
     灵芝药效基因启动子的克隆
短句来源
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  actin gene promoter
The genetic construct was similar to ours and contained the reporter lacZ gene under the control of the chicken b-actin gene promoter.
      
The smooth muscle alpha-actin gene promoter is differentially regulated in smooth muscle versus non-smooth muscle cells.
      


To raise carp's resistance to hemorrhage,a large amount of transgenic grass carp was obtained by transfering hu IFN α gene which was cloned under the control of the β actin gene promoter of grass carp to the cytula of carp in the way of microinjection. ELISA detection of the gene expression of the transgenic grass carps was carried out by using the serum as samples.158 individuals were tested to be positive with a detectable hu IFN α expression level in 672 transgenic fish. The detectable ratio is 23.51%...

To raise carp's resistance to hemorrhage,a large amount of transgenic grass carp was obtained by transfering hu IFN α gene which was cloned under the control of the β actin gene promoter of grass carp to the cytula of carp in the way of microinjection. ELISA detection of the gene expression of the transgenic grass carps was carried out by using the serum as samples.158 individuals were tested to be positive with a detectable hu IFN α expression level in 672 transgenic fish. The detectable ratio is 23.51% and the highest is 1 450 pg/mL in serum. The expression level of the transformed gene changes in different seasons and development stages.

为提高草鱼对出血病的抗性 ,采用显微注射法 ,将克隆在鲤 β-肌动蛋白基因启动子下游的人 α-干扰素基因转移到草鱼受精卵中 ,获得了大量转基因个体 .抽取转基因鱼血浆 ,以酶联免疫吸附法检测了转基因草鱼中人α-干扰素基因的表达情况 .从 6 72尾转基因草鱼中检测出 15 8尾有 α-干扰素的表达 ,阳性率为 2 3.5 1% ,其中表达水平最高个体血浆中α-干扰素质量浓度为 145 0 pg/ m L .基因表达量随季节和个体发育时期有所变化

Total DNA from foot muscle of female individial in Haliotis discus hannai was extracted by routine method. Actin gene promoter was amplified by PCR technique. A recombinant was obtained after cloning and screening. Its length is 511 bp. The result shows after the sequence is analysed: The homologous series of nuleotides sequence between Haliotis discus hannai and Haliotis rufescens is 95%. The CC base content of Haliotis discus hannai is 38. 93%. This value is 59. 2% lower than that of Haliotis rufescens. The...

Total DNA from foot muscle of female individial in Haliotis discus hannai was extracted by routine method. Actin gene promoter was amplified by PCR technique. A recombinant was obtained after cloning and screening. Its length is 511 bp. The result shows after the sequence is analysed: The homologous series of nuleotides sequence between Haliotis discus hannai and Haliotis rufescens is 95%. The CC base content of Haliotis discus hannai is 38. 93%. This value is 59. 2% lower than that of Haliotis rufescens. The sequence includes expression of control elements whose high level of conservation can be seen, i. e. four TATA boxes and one CAAT box.

从皱纹盘鲍雌性个体的足部肌肉提取总DNA后,通过聚合酶连式反应(PCR)技术扩增得到一个扩增产 物。经克隆、筛选、确定重组子产物。测序得到了长度为511bp的启动子片段。分析测序结果发现,皱纹盘鲍 肌动蛋白基因启动子DNA序列与目前已知的红鲍相应序列的相似度为95%;OC碱基含量为38.93%,较红 鲍的低(59.2%);所得序列含有高度保守的基本表达调控元件,即一个CAAT框和四个:TATA框。

The coding sequence of hu IFN α gene is cloned under the control of Carp β actin gene promoter. Thus a fish constantly expression of hu IFN α gene recombinant is constructed. Southern blot and sequencing results show that the recombinant molecule is correctly constructed. The recombinant gene is transferred into fertilized zygotes of grass carp via microinjection. And hu IFN α is detected out in 20% of transgenic fish by ELISA.

通过分子重组 ,将人α 干扰素 (hu IFN α)基因编码序列克隆到鲤鱼β 肌动蛋白基因启动子下游 ,构建成能在鱼体内组成型表达hu IFN α的基因重组分子。经限制性酶切分析、Southern杂交和序列测定 ,证明重组分子构建的正确性。采用显微注射法将这一重组基因转化草鱼受精卵 ,获得了表达人α 干扰素的转基因草鱼

 
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