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神经毒素基因
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  neurotoxin gene
     Study on Fusion Cloning, Expression of cry1Ac Gene from Bacillus Thuringiensis with a Neurotoxin Gene hwtx-Ⅰ and Bioassay Analysis of Fusion Protein
     苏云金杆菌cry1Ac基因与神经毒素基因hwtx-Ⅰ的融合克隆表达及其融合蛋白活性研究
短句来源
     Rapid detection of type E botulinal neurotoxin gene by polymerase chain reaction
     聚合酶链式反应快速检测E型肉毒神经毒素基因
短句来源
     Methods We used a pair of synthetic oligonucleotides fragment as primers to amplify DNA fragment, a section of botulinum neurotoxin gene of type A, B, E, F and G and length 264bp, and detected the cultivation liquid of 2 food poisoning samples and the bacteria separated from these samples.
     方法用1对人工合成的寡核苷酸引物护增A、B、E、F和G型肉毒神经毒素基因的一段264bp的DNA片段,并对2个食物中毒样品的增菌产毒培养液及分离的菌株进行检测。
短句来源
     Cloning and Sequence Analysis of Neurotoxin Gene from Sea Anemone
     海葵神经毒素基因的克隆和序列分析
短句来源
     The molecular weight and the digestion pattern with restriction endonuclease of the amplified product of the strain LCL001 were all coincident with those of Clostridium botulinum type B.It could be regarded as a strain of Clostridium botulinum type A which contained unexpressed type B neurotoxin gene,or signed it type A(B).
     LCL001的扩增产物其分子量以及限制性内切酶消化产物和B型肉毒梭菌的扩增产物完全一致,认为该株菌中带有不表达的B型肉毒神经毒素基因,应为A(B)型。
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  “神经毒素基因”译为未确定词的双语例句
     Cloning and Expression of Buthus martensiiKarsch Scorpion Toxin Gene (BmK IT_3) in Escherichia coli
     东亚钳蝎神经毒素基因BmK IT_3的克隆及其在大肠杆菌中的表达
短句来源
     PCR for detection of botulinum neruotoxin genes in botulinum neurotoxin A ,B ,E ,F and G producing Clostridia
     PCR 检测产 A、B、E、F 和 G 型肉毒神经毒素梭菌的神经毒素基因
短句来源
     The Synthesis of Insect-specific Neurototin Gene tox34 and Acquirement of Transgenic Tobacco
     昆虫特异性神经毒素基因tox34的合成及转基因烟草的获得
短句来源
     At present, researches on appliance of Bacillus thuringiensis are mainly about constructing more toxic recombinant protein and recombinant strain by the technology of genetic engineering such as site-directed mutation, constructing fusion gene, constructing transgenic plants of Bt toxin gene and so on.
     在苏云金芽孢杆菌(Bacillus thuringiensis,简称Bt)应用研究上,主要利用基因工程技术来构建更高毒力的重组蛋白和重组菌株,如定点突变研究,融合基因研究,转基因植物研究等方面的研究。 为了构建更高毒力,杀虫谱更广的工程菌株,本研究利用crylAc基因和神经毒素基因hwtx-Ⅰ构建融合基因来提高晶体蛋白的杀虫毒力。
短句来源
     Therefore,it suggests that the PCR system is specific and sensitive for identification of Clostridium botulinum type A and rapid diagnosis of type A botulism.
     由此可见,该PCR扩增系统用于A型肉毒神经毒素基因的检测具有灵敏度高,特异性强等特点,为A型肉毒梭菌的鉴定及肉毒中毒的快速诊断提供了一种新的手段。
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  相似匹配句对
     4 new genes were obtained.
     U基因
短句来源
     Cloning and Sequence Analysis of Neurotoxin Gene from Sea Anemone
     海葵神经毒素基因的克隆和序列分析
短句来源
     Gene identification and genotyping of clostridial neurotoxins
     梭菌属神经毒素基因鉴定与分型
短句来源
     The Gene of All Fears
     害怕的基因
短句来源
     THE ANALGESIC EFFECT OF NEUROTOXIN FROM COBRA ( NAJA NAJA ) VENOM
     眼镜蛇神经毒素的镇痛作用
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  neurotoxin gene
Sequencing and phylogenetic analysis of neurotoxin gene from an environmental isolate of Clostridium sp.: comparison with other
      
The neurotoxin gene fragments were cloned in Escherichia coli and sequenced.
      
The phylogenetic interrelationship between the neurotoxin gene of Clostridium sp.
      
Gene Organization and Sequence Determination of the Two Botulinum Neurotoxin Gene Clusters in Clostridium botulinum Type A(B) St
      
Neurotoxin Gene Clusters in Clostridium botulinum Type A Strains: Sequence Comparison and Evolutionary Implications
      
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The apparent causative organism from the food (soused paste made of soybean and wax gourd) causing an outbreak of type E food borne botulism was isolated.The biological and biochemical characteristics of the isolate were examined.It had the ability to produce type E botulinal toxin,and the type E neurotoxin gene was also definited by polymerase chain reaction(PCR).But many biochemical properties of the isolate are quite different from Clostridium botulinum and corresponded to Clostridium butyricum.The food...

The apparent causative organism from the food (soused paste made of soybean and wax gourd) causing an outbreak of type E food borne botulism was isolated.The biological and biochemical characteristics of the isolate were examined.It had the ability to produce type E botulinal toxin,and the type E neurotoxin gene was also definited by polymerase chain reaction(PCR).But many biochemical properties of the isolate are quite different from Clostridium botulinum and corresponded to Clostridium butyricum.The food borne botulism caused by neurotoxigenic Clostridium butyricum was primarily reported in the world.

对某卫生防疫站委托本研究室分离病原菌的一份引起肉毒中毒的食品一"黄豆冬瓜酱"进行检测和病原菌的分离,从中再次检出了E型肉毒毒素并分离到一产毒菌种,对该菌种的生物学及生化学特性进行检查,并检测其毒素基因(PCR试验)。结果:该分离菌能产生E型肉毒毒素,PCR检测结果也证明其具有E型肉毒神经毒素基因,但其多项生化特性与E型肉毒梭菌有明显差异,而与酪酸梭菌完全一致。结果说明该分离菌系产生E型肉毒毒素的酪酸梭菌,而非E型肉毒梭菌。由酪酸梭菌引起的食物中毒型肉毒中毒并从中毒食品中分离到该病原菌,这在国际上尚属首次报告。

Botulism is a neuroparalytic disease caused by the neurotoxin produced from Clostridium botulinum. The rapid diagnosis and typing of botulism is significant for the treatment of botulism and decreasing the mortality.In this study,a polymerase chain reaction (PCR) was developed for detection of neurotoxin gene of Clostridium botulinum type A using a set of oligonucleotide primer which designed from the nucleotide sequence of the light chain of type A neurotoxin gene to amplify a fragment of 472bp.Sixty...

Botulism is a neuroparalytic disease caused by the neurotoxin produced from Clostridium botulinum. The rapid diagnosis and typing of botulism is significant for the treatment of botulism and decreasing the mortality.In this study,a polymerase chain reaction (PCR) was developed for detection of neurotoxin gene of Clostridium botulinum type A using a set of oligonucleotide primer which designed from the nucleotide sequence of the light chain of type A neurotoxin gene to amplify a fragment of 472bp.Sixty eight strains belonging to 10 clostridial species were detected by the PCR.The results revealed that all of the 20 strains of Clostridium botulinum type A were positive and the others were all negative in PCR.The fragments amplified from several strains of Clostridium botulinum type A were digested with restriction endonuclease to identify the amplified products,and the digestion patterns were in agreement with the sizes estimated from the sequence data.Clear fragment could be obtained from 3×10 3 cells and as little as 10pg of DNA could be detected with phenol chloroform extracts in this PCR.Therefore,it suggests that the PCR system is specific and sensitive for identification of Clostridium botulinum type A and rapid diagnosis of type A botulism.

建立快速诊断及定型神经毒素对肉毒中毒的治疗、降低死亡率具有十分重要的意义。用一对A型肉毒梭菌特异的寡核苷酸引物,扩增A型肉毒神经毒素基因轻链区域一段472bp的DNA片段,对梭状芽胞杆菌属的10种68株菌进行了鉴定,并对PCR检测A型肉毒神经毒素基因的灵敏度进行了检查。结果表明,所有20株A型肉毒梭菌PCR扩增均为阳性,其余各型肉毒梭菌及其它各种梭菌均为阴性。扩增产物经限制性内切酶分析与预期的酶切片段一致。用溴化乙锭对扩增产物染色,可从3×103个细菌中检测出A型肉毒神经毒素基因。用酚-氯仿抽提A型肉毒梭菌全菌体DNA进行PCR扩增,可从10pg的DNA中得到清晰的扩增产物。由此可见,该PCR扩增系统用于A型肉毒神经毒素基因的检测具有灵敏度高,特异性强等特点,为A型肉毒梭菌的鉴定及肉毒中毒的快速诊断提供了一种新的手段。

A polymerase chain reaction(PCR) method was developed for detection of Clostridium botulinum type B neurotoxin gene by using a pair of oligonucleotide primers which were designed from the nucleotide sequence of the light chain of type B neurotoxin gene to amplify a fragment of 253bp.Twenty two strains of Clostridium botulinum type B were all positive in PCR and mouse lethal test.Sensitivity of the PCR was determined by using Clostridium botulinum type B CMCC(B) 64352.The results showed that a clear...

A polymerase chain reaction(PCR) method was developed for detection of Clostridium botulinum type B neurotoxin gene by using a pair of oligonucleotide primers which were designed from the nucleotide sequence of the light chain of type B neurotoxin gene to amplify a fragment of 253bp.Twenty two strains of Clostridium botulinum type B were all positive in PCR and mouse lethal test.Sensitivity of the PCR was determined by using Clostridium botulinum type B CMCC(B) 64352.The results showed that a clear amplified fragment could be obtained from only 60 organisms of Clostridium botulinum type B.The specificity of the PCR was determined by using 53 strains belonging to other types of Clostridium botulinum and other clostridial species.All strains except LCL001,one of Clostridium botulinum type A,were negative in the PCR.The molecular weight and the digestion pattern with restriction endonuclease of the amplified product of the strain LCL001 were all coincident with those of Clostridium botulinum type B.It could be regarded as a strain of Clostridium botulinum type A which contained unexpressed type B neurotoxin gene,or signed it type A(B).Therefore,it suggests that the PCR system is excellent for identification of Clostridium botulinum because of not only its specificity and sensitivity,but also the feasibility of finding out the silent type B neurotoxin gene in other types of Clostridium botulinum .

本研究用一对B型肉毒神经毒素基因特异的寡核苷酸引物,扩增B型基因轻链区域一段253bp的DNA片段,对22株B型肉毒梭菌进行了鉴定,所试22株B型肉毒梭菌其PCR均为阳性。用B型肉毒梭菌CMCC(B)64352对PCR的检测灵敏度进行检查,可从60个细菌中得到明显的扩增产物。用其它各型肉毒梭菌及其它梭状芽胞杆菌共53株对PCR的特异性进行了检测,除一株A型肉毒梭菌(LCL001)PCR为阳性外,其余菌株均为阴性。LCL001的扩增产物其分子量以及限制性内切酶消化产物和B型肉毒梭菌的扩增产物完全一致,认为该株菌中带有不表达的B型肉毒神经毒素基因,应为A(B)型。由此可见,该PCR扩增系统不仅具有灵敏度高,特异性强等特点,而且可检测出不表达的B型肉毒神经毒素基因,用于肉毒梭菌的鉴定具有其它方法不可比拟的优点。

 
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