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生产基因
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     In this study,a new plasmid pSY200-14 is constructed bygene-recombinatoin Technology,which has a genetic system of phenylalanine production,by using plasmid pSY130-14 and pSY16.The pSY130-14 contains pheA~(FH) and aroF~(FR),the feedback inhibition resistant phenylalanine-production genes,and a temperature-sensitive repressor cI_(857) ,whereas pSY16 possesses a partition system and is a low copy plasmid.
     本研究应用具有苯丙氨酸生产基因 pheA~(FR)、aroF~(FR)、CI_(857) 的质粒 pSY130~14和质粒 pS Y16,重组构建了具有苯丙氨酸生产基因系统的新质粒 pSY200-14。 然后,使其转化到大肠菌 AT2471中,育成了基因重组菌株 AT2471/pSY200-14。
短句来源
     Isolation and Purification of Recombinant Grass Carp Growth Hormone Produced in Escherichia coli
     大肠杆菌生产基因重组草鱼生长激素的分离纯化
短句来源
     pBV-220 vector is constructed for high expressing gene in E. coli by the scientist in China.
     pBV-220表达载体,是国内科学家自己构建的原核高效表达载体,目前在国内被广泛应用于生产基因重组药物。
短句来源
     And, as human embryonic kidney cell line, HEK293 cells has become an appropriate system to produce both gene therapy vectors and therapeutic proteins, the work presented herein focused in CFIC of HEK293 cells.
     鉴于人胚肾细胞系HEK293细胞已被广泛地用作生产基因治疗载体和重组蛋白的哺乳动物细胞,本论文研究围绕HEK293细胞的无载体固定化培养展开。
短句来源
     The results had shown that human RANTES gene was successfully integrated and expressed in tobacco cells, which provided a basis to a large-scale production of gene recombinant medicines in plant cells.
     结果表明,人RANTES基因已在烟草细胞中成功地整合与表达,这为在植物细胞中大量生产基因重组药物打下了基础。
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     4 new genes were obtained.
     U基因
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     The Major Gene's Application in Animal Husbandry Producing
     主效基因在畜牧生产上的应用
短句来源
     Producing knowledge
     知识的生产
短句来源
     The Gene of All Fears
     害怕的基因
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     Production of Soybean Milk
     豆乳的生产
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  produce gene
This region of the F-linked gene contains an LRR region that is characterized by two alternatively spliced products which produce gene products with either a four-repeat or a ten-repeat LRR.
      
In this study, RAW264.7 murine macrophage cells were treated with H2O2 and cDNA microarray technique was used to produce gene expression profiles.
      
These results suggest that three different rye chromosomes produce gene products which can interact with the wheat malate dehydrogenase regulatory system.
      
However, development of tumors also requires cell proliferation in concert with DNA damage to produce gene mutations.
      


In this study,a new plasmid pSY200-14 is constructed bygene-recombinatoin Technology,which has a genetic system of phenylalanine production,by using plasmid pSY130-14 and pSY16.The pSY130-14 contains pheA~(FH) and aroF~(FR),the feedback inhibition resistant phenylalanine-production genes,and a temperature-sensitive repressor cI_(857) ,whereas pSY16 possesses a partition system and is a low copy plasmid.Subsequent xperiments have shown that the recombinant AT2471/pSY200-14 newly constructed is more stable than...

In this study,a new plasmid pSY200-14 is constructed bygene-recombinatoin Technology,which has a genetic system of phenylalanine production,by using plasmid pSY130-14 and pSY16.The pSY130-14 contains pheA~(FH) and aroF~(FR),the feedback inhibition resistant phenylalanine-production genes,and a temperature-sensitive repressor cI_(857) ,whereas pSY16 possesses a partition system and is a low copy plasmid.Subsequent xperiments have shown that the recombinant AT2471/pSY200-14 newly constructed is more stable than the original strain AT2471/pSY130-14. In other words,the former keep the stability as high as 100% under selective pressure

本研究应用具有苯丙氨酸生产基因 pheA~(FR)、aroF~(FR)、CI_(857) 的质粒 pSY130~14和质粒 pS Y16,重组构建了具有苯丙氨酸生产基因系统的新质粒 pSY200-14。然后,使其转化到大肠菌 AT2471中,育成了基因重组菌株 AT2471/pSY200-14。试验表明,新菌株质粒稳定性比原菌株有较大的提高。在2. 5升通气搅拌罐进行苯丙氨酸发酵试验,在搅拌转速850rpm、通气速率1. 0VVM,38. 5℃和 pH7. 0的条件下,发酵48小时苯丙氨酸生成量达14. 2g/L,比原菌株 AT2471/pSY130-14增产11. 8%。

Gene transfer goats produced by the method of microinjection foreign gene into pronuclear were made integrated detection with a novel nonradioactive DNA labeling method, namely DNA probe labeling with Digoxigenin. The experiment result show that the foreign gere integrated in gene transfer animal's genomic DNA can be detected.

对采用外源基因原核显微注射法生产的基因转移山羊,用一种新的DNA非同位素标记法即地高辛配基标记DNA探针作外源基因整合检测.结果表明:地高辛标记核酸探针能检测出基因转移动物基因组中整合的外源基因.

Experiments of yeast electroporation transformation were performed in Bio-Rad Gene Pulser. The best conditions are 5 ky/cm, 25 μF and 200Ω, incubation time after electroporation is two hours.When survivor rate was 46 percent,the highest transformation frequency,over 106 transformants per μg DNA was obtained.With lower concentration of plasmid DNA we obtained higher transformation frequency.Compared with protoplast procedure and LiAC procedure using same plasmid DNA and same recipient we only obtained 2×104 and...

Experiments of yeast electroporation transformation were performed in Bio-Rad Gene Pulser. The best conditions are 5 ky/cm, 25 μF and 200Ω, incubation time after electroporation is two hours.When survivor rate was 46 percent,the highest transformation frequency,over 106 transformants per μg DNA was obtained.With lower concentration of plasmid DNA we obtained higher transformation frequency.Compared with protoplast procedure and LiAC procedure using same plasmid DNA and same recipient we only obtained 2×104 and 3.5×102 transformants per μg DNA,respetively.Electro poration procedure is the most convenient and high efficient method.

用Bio-Rad生产的基因脉冲仪进行酿酒酵母电击转化实验,得到的最适条件为:5kv/cm25μF和200Ω。电击后涂布前的培养时间为2小时。电击后细胞存活率为46%时,每微克质粒DNA得到106以上的转化子。用相同的质粒和受体菌进行原生质体法和醋酸锂法比较实验,转化率分别为2×104和3.5×102个转化子/μgDNA。电击转化是最方便易行和高效率的方法。

 
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