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骨髓msc
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  bone marrow msc
     Results Bone marrow MSC proliferated as undifferentiated cells in culture for 15 passages and (2-3)×10 12 cells were obtained.
     结果 成人骨髓MSC在体外扩增 15代可获得 ( 2~ 3 )× 10 12 个细胞。
短句来源
     The proliferation and differentiation of the adult human bone marrow MSC were examined by phase-contrast microscope, transmission electron microscope, MTT, the determination of alkaline phosphatase (ALP) activities and ALP staining.
     利用倒置光学显微镜、透射电镜、四甲基偶氮唑盐(MTT)比色、碱性磷酸酶(ALP)染色、ALP活性测定等方法研究成人骨髓MSC增殖和分化情况。
短句来源
     And then the different concentrations of rhTGF-β1 were added into the basic induction medium including β-glycerophosphate, Vitamin C, Dexamethasone to induce the adult human bone marrow MSC to differentiate into osteoblasts in vitro.
     采用基础诱导培养液[地塞米松、β-甘油磷酸钠、L-抗坏血酸加不同浓度的重组人转化生长因子-β1(recombinanthumantransforminggrowthfactor-beta1,rhTGF-β1)]诱导成人骨髓MSC体外分化为成骨细胞。
短句来源
     METHODS: Bone marrow MSC was cultured with DMEM media (10% fetal calf serum) 4-6 passages, and induced by HGF (10 μg/L) for 30 d. Automatical beating of the differentiated cells was observed daily with transverse microscopy, or under condition of 0.1% isoproterenol or cal (cium-deprived) incubation.
     方法 :分离SD大鼠骨髓MSC ,培养至 4 - 6代后 ,添加HGF(终浓度 10 μg/L)持续培养 30d ,倒置显微镜下动态观察分化细胞的自律性搏动和对 0 1%异丙肾上腺素和无Ca2 + 孵育液的反应 ,并行心肌肌凝蛋白表达鉴定。
短句来源
     The results showed that the osteoblasts induced from bone marrow MSC in constructed two-demensional culture system displayed more significant support effect on survival of hematopoietic stem/progenitor cells from umbilical cord blood (UCB) ex vivo, compared with other culture systems, especially on long term HSCs survival ex vivo.
     结果发现:在所构建的造血干/祖细胞(HSPC)二维培养体系中,骨髓MSC诱导成骨细胞对于脐血造血干/祖细胞培养的支持作用显著优于其他各组培养体系,尤其对长期造血干细胞(longterm-HSC)体外生存有更为明显地支持作用。
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  bone marrow derived msc
     the adult bone marrow derived MSC is useful in bioengineering, cell and gene therapy;
     成人骨髓MSC在组织工程、细胞治疗、基因治疗等领域具有广阔的临床应用前景;
短句来源
     Results Flow cytometry analysis showed that fetal bone marrow derived MSC exhibited the characteristics of mesenchymal cells, MSC had an active proliferative ability in primary and passage cultures.
     结果 流式细胞术证明 ,MSC具有间充质细胞的特征。 原代及传代培养显示 ,胎儿骨髓MSC具有活跃增殖的能力。
短句来源
     Conclusion Fetal bone marrow derived MSC has a great feasibility as the seed cells for tissue engineering reconstruction.
     结论 胎儿骨髓MSC作为组织工程重建的种子细胞具有较强的可行性
短句来源
     The proliferative and multilineage differentiation potential of the bone marrow derived MSC from the fetus is higher than that from the adult, but the adherent ability of the MSCs from the adult is higher than that from the fetus.
     成人骨髓MSC的粘附功能则强于胎儿。 结论 从成人及胎儿骨髓中可分离培养出MSC ,在体外有效扩增且保持其低分化状态和多向分化能力。
短句来源
     Methods:Human bone marrow derived MSC and HSC were used as the donor.
     方法:分离培养水囊引产4~5个月龄胎儿来源的骨髓MSC和脐血CD34+造血干/祖细胞(HSC),作为供体细胞。
短句来源
  “骨髓msc”译为未确定词的双语例句
     Results The mdr1 gene was expressed stably in transfected group,and there were 33.9% cells expressing P-g170 while 0.7% in control group.
     结果mdr1基因导入骨髓MSC后稳定表达P-g170的阳性细胞百分率为33.9%,对照组为0.7%;
短句来源
     Results: Under optimized conditions,the MSCs expressed CD29、CD44、CD105、 CD105 and CD166,but not antigens of hematopoietic CD15,CD34,CD45 and not antigens related to GVHD such as HLA-DR、CD80、CD86、CD40、CD40L. Exposure of these cells to osteogenic inductive agents resulted in an increase in expression of alkaline phosphatase and the appearance of hydroxyapatite nodules.
     流式细胞仪检测P3代细胞结果显示:人胎骨髓MSC表达CD15、CD29、CD44、CD105、CD106和CD166,不表达造血细胞标志CD34、CD45,不表达与GVHD相关的HLA-DR、CD80、CD86、CD40、CD40L。
短句来源
     The primary culture time of group B was shorter than other groups. The duration of passage 1 (P1 ) was 5.5 days, and the duration of P10 was 33 days, after P10 culture, (5.19±2.15)×10 10 MSCs were obtained from 8×106MNC of this group.
     原代培养B组骨髓MSC含量较多,贴壁时间早,传代时间短,P0至P1时间为5.5天,传至P10需33天,8×106MNC培养至P10时MSC数达(5.19±2.15)×1010;
短句来源
     The results showed that bone marrow-derived MSCs from MM patients were homogenously positive for CD29, CD73, CD166 and HLA-ABC and negative for hematopoietic cell marker CD45 and endothelial cell marker CD31, the phenotype of which was similar to that of marrow counterparts from normal adults.
     结果表明与正常人骨髓MSC比较,MM患者骨髓MSC的表型无改变,均一表达CD29、CD73、CD166和HLA-ABC,不表达CD45和CD31;
短句来源
     FACS results showed that bone marrow MSCs did not express antigens CD34 and CD 11 a, and expressed CD29 and CD71. Passage 2, 6 and 10 of bone marrow MSCs were induced to differentiate into neuroncytes.
     流式细胞仪检测结果显示,骨髓MSC不表达CD34、CD11a,强表达CD29,弱表达CD71。
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  bone marrow derived msc
Analysis of islet like clusters from bone marrow derived MSC from non-diabetic patients.
      


Bone marrow mesenchymal stem cells (MSCs) are multipotential progenitors of connective tissues and bone marrow stroma as well, which implies the modulatory function of MSCs in hematopoiesis. To clarify the contributions of MSCs to hematopoiesis, the methods for isolation and expansion of MSCs were established and long term bone marrow cultures were performed using irradiated MSCs as the feeder layer. The results here showed that CD34 + cells from cord blood formed hematopoietic foci adherent to the monolayer....

Bone marrow mesenchymal stem cells (MSCs) are multipotential progenitors of connective tissues and bone marrow stroma as well, which implies the modulatory function of MSCs in hematopoiesis. To clarify the contributions of MSCs to hematopoiesis, the methods for isolation and expansion of MSCs were established and long term bone marrow cultures were performed using irradiated MSCs as the feeder layer. The results here showed that CD34 + cells from cord blood formed hematopoietic foci adherent to the monolayer. Furthermore, colony forming cells remained in the coculture of 5 weeks, indicating the maintenance of long term culture initiating cells (LTC IC). Flow cytometry analysis showed that about 1% of the hematopoietic cells in the culture were positive for CD34 and around 15% were CD41a positive. It is clear that MSCs maintain LTC IC in vitro and promote differentiation of hematopoietic progenitors especially into megakaryocytic lineage. The preliminary results here demonstrate that MSCs residing in the bone marrow might be a crucial cellular component in the hematopoietic microenvironment.

体内的稳态造血依赖于复杂而完整的骨髓造血微环境系统 ,其中的细胞成分是该系统的关键。存在于骨髓中的间充质干细胞 (MSCs)是成纤维细胞、内皮细胞、成骨细胞、脂肪细胞等多种骨髓基质细胞的前体细胞 ,在造血调控中可能具有一定的作用。本研究首先建立了成人骨髓MSCs的分离及体外培养的方法 ,并应用长期骨髓细胞培养体系 ,观察了MSCs滋养层体外维持长期培养启动细胞 (LTC IC)的能力及其促进造血细胞分化的功能。结果显示 :①脐带血来源的CD34+细胞粘附于滋养层上形成造血灶 ,表明MSCs可形成与骨髓基质细胞相似的体外造血微环境 ;②共培养 5周后造血细胞仍具有体外集落形成能力 ,说明MSCs具有维系LTC IC的能力 ;③流式细胞术分析显示 ,体外培养 5周后约 1%悬浮细胞表达CD34,15 %细胞CD4 1a阳性 ,提示MSCs促进造血细胞向巨核系细胞分化。研究证明MSCs可能是造血微环境中的重要细胞成分

Objective To study the feasibility of constructing tissue engineered cartilage by differentiated rabbit bone marrow mesenchymal stem cells(MSC) cultured in vitro and in vivo . Methods The MSC were isolated from the nucleated cells fraction of autologous bone marrow by density gradient centrifuge, and then induced into chondrogenic differentiation by adding dexamethasone, transforming growth factor β 1(TGF β 1) and ascorbic acid in vitro . After 3 weeks, some cells turned to round shape and secreted...

Objective To study the feasibility of constructing tissue engineered cartilage by differentiated rabbit bone marrow mesenchymal stem cells(MSC) cultured in vitro and in vivo . Methods The MSC were isolated from the nucleated cells fraction of autologous bone marrow by density gradient centrifuge, and then induced into chondrogenic differentiation by adding dexamethasone, transforming growth factor β 1(TGF β 1) and ascorbic acid in vitro . After 3 weeks, some cells turned to round shape and secreted metachromatic matrix. The cartilaginoid grafts composed of chondrogenic MSC. Bovine type Ⅰ collagen and human fibrin were cultured within the chondrogenic medium for 2 weeks in vitro or transplanted subcutaneously adjacent to the knee joint for 3 weeks in vivo . Results The most cells in the grafts were degenerated and disappeared after cultured in vitro . But the residual cells were survival and secreted metachromatic staining proteoglycan with toluidine blue, which was characteristic cartilage matrix. The grafts developed into matured cartilage tissue assessed by histological examination after 3 weeks of transplantation in vivo . Conclusion MSC can be used as functional cells to constructing tissue engineered cartilage.

目的 采用组织工程方法 ,以培养后的兔骨髓间质干细胞 (MSC)制成人工软骨培养物 ,经体内外培养后发育出成活的软骨组织。方法 抽取兔骨髓液经密度梯度离心得到单个核细胞 ,再经体外分离、培养获得兔骨髓 MSC。向 MSC培养液内加入地塞米松、转化生长因子 -β1 (TGF-β1 )和维生素 C进行软骨起源诱导培养 3周 ,部分细胞开始转变为圆形并分泌基质。将诱导后的细胞与牛 型胶原及人纤维蛋白按一定的比例混合 ,制成软骨样的培养物并分别做体内外培养。结果 体外培养 2周后 ,培养物内大部分细胞已萎缩消失。但剩余的少量细胞成活 ,形成类似的软骨陷窝并分泌甲苯胺蓝异染的软骨基质。体内移植培养 3周后 ,培养物已发育成颗粒状成熟的软骨组织。结论 骨髓间质干细胞可用于组织工程软骨组织的构建 ,是一种非常有前途的人工软骨组织构建中的功能细胞。

Objective To observe the main biological characteristics of marrow-derived mesenchymal stem cells (MSCs) during the passage cultivation under the condition for chondrocyte culture. Methods The marrow-derived MSCs were isolated from young rabbits and cultivated in the condition for culturing chondrocytes. The changes of morphology, growth and proliferation, synthesis of collagen typeⅠ and Ⅱ and aggrecan, and activity of alkaline phosphatase (ALP) were observed during the passage cultivation of MSCs. Results...

Objective To observe the main biological characteristics of marrow-derived mesenchymal stem cells (MSCs) during the passage cultivation under the condition for chondrocyte culture. Methods The marrow-derived MSCs were isolated from young rabbits and cultivated in the condition for culturing chondrocytes. The changes of morphology, growth and proliferation, synthesis of collagen typeⅠ and Ⅱ and aggrecan, and activity of alkaline phosphatase (ALP) were observed during the passage cultivation of MSCs. Results 1) Primary MSCs proliferated in visible symmetric colonies with long-spindle shape. The morphological characteristics of marrow-derived MSCs had no change during passaging, and its homogenous rose with passaging. 2) Every passage cells showed positive staining of collagen typeⅠ, and negative of collagen typeⅡ. 3) The cells had negative staining of ALP, and weak heterochromatic to toluidine blue. The content of sulfate glycosaminoglycans (GAG) was very little in extracellular matrix. Conclusion The biological characteristics of MSCs in the basic condition for culturing chondrocytes are the same with in the regular low-glucose condition for culturing MSCs.

目的 观察骨髓间充质干细胞 (Mesenchymalstemcells ,MSCs)在软骨细胞培养条件下传代培养的主要生物学特性。方法 梯度离心分离幼兔骨髓有核细胞 ,培养分离MSCs并进行传代培养 ,观测在体外软骨细胞培养条件下MSCs形态、生长特点及Ⅰ型和Ⅱ型胶原、蛋白多糖聚集体合成分泌以及碱性磷酸酶活性等方面的变化。结果 ①MSCs原代细胞呈可见的均匀分布的簇状生长 ,呈长梭形 ,在传代培养中形态特性无明显变化、均质性明显提高 ;②各代Ⅰ型胶原免疫组化均为阳性 ,Ⅱ型胶原免疫组化均为阴性。③在各代培养中碱性磷酸酶染色均为阴性 ,对甲苯胺蓝存在极弱的异染性反应 ;④细胞外基质中硫酸糖胺多糖含量亦很低。结论 传代培养中骨髓MSCs保持了常规低糖培养条件下的基本生物学特点 ,说明常用的软骨细胞基础培养条件同样适合骨髓MSCs的培养 .

 
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