All of the compounds was carried out MTT assay for determine anticancer activity in vitro. As a result, compounds 5, 4c-4j, 6e-6j, 7d-7g showed anticancer activity (dosage ≤40 ug/ml, inhibitory rate ≥ 50%).
Some new benzenesulfonamides, disubstituted sulfonylureas, and sulfonylthioureas substituted basically with 3-(2-thienyl or 3-pyridyl)-indeno[1,2-c]pyrazol(in)e counterpart were synthesized to be evaluated for their in vitro antitumor activity.
According to the protocol of the National Cancer Institute (NCI) in vitro disease-oriented human cells screening panel assay, 13 compounds showed promising broad spectrum antitumor activity.
Human LAK cells were prepared by culturing normal human peripheral blood mononuclear cells (PBMC) with or without rIL-2 and assayed for T cell surface markers as well as anti-tumor activity against PGin vitro and in nude mice.
A-LAK cells were treated with the supernatant of SMMC7721 cells which had been pretreated with PA and the changes of the proliferation and anti-tumor activity of A-LAK cells were investigated.
As important, these apoptosis-resistant γδ-T cells appear to retain major histocompatibility complex-unrestricted (innate) anti-tumor activity against a wide variety of human tumor cells both in vitro and in vivo.
The procedures developed for modification of functional groups in the adamantane skeleton provide a possibility of synthesis of compounds with potential anticancer activity.
A high-performance liquid chromatographic method is reported for the resolution of the enantiomers of a series of fused γ-lactams (2,7-diaza-3-oxo[3.3.0]octan-6-ones) with probable anti-HIV and anticancer activity.