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恶性疟原虫
相关语句
  plasmodium falciparum
    STUDIES ON (α-~(32)P)—dATP LABELLED DNA PROBE OF PLASMODIUM FALCIPARUM IN DIAGNOSIS OF FALCIPARUM MALARIA
    用[α-~(32)P]—dATP标记恶性疟原虫DNA探针诊断恶性疟疾的研究
短句来源
    Studies on Nonisotope-labeling DNA Probes and Its Hybridization with Plasmodium falciparum Nucleic Acids
    非同位素标记DNA探针与恶性疟原虫核酸杂交的实验研究
短句来源
    DETECTION OF PLASMODIUM FALCIPARUM FROM DRIED BLOOD SPOTTED FILTER PAPER BY NESTED PCR
    套式PCR检测滤纸干血滴中恶性疟原虫的研究
短句来源
    APPLIED STUDY OF GOLD-IMMUNOCHROMATOGRAPHY ASSAY FOR HRP ⅡANTIGEN IN PLASMODIUM FALCIPARUM
    胶体金层析法检测恶性疟原虫HRPII抗原的应用研究
短句来源
    Expression and Immunocompetence Characterization of Plasmodium falciparum Lactate Dehydrogenase
    恶性疟原虫乳酸脱氢酶的表达及免疫活性鉴定
短句来源
更多       
  plasmodium falciparum
    STUDIES ON (α-~(32)P)—dATP LABELLED DNA PROBE OF PLASMODIUM FALCIPARUM IN DIAGNOSIS OF FALCIPARUM MALARIA
    用[α-~(32)P]—dATP标记恶性疟原虫DNA探针诊断恶性疟疾的研究
短句来源
    Studies on Nonisotope-labeling DNA Probes and Its Hybridization with Plasmodium falciparum Nucleic Acids
    非同位素标记DNA探针与恶性疟原虫核酸杂交的实验研究
短句来源
    DETECTION OF PLASMODIUM FALCIPARUM FROM DRIED BLOOD SPOTTED FILTER PAPER BY NESTED PCR
    套式PCR检测滤纸干血滴中恶性疟原虫的研究
短句来源
    APPLIED STUDY OF GOLD-IMMUNOCHROMATOGRAPHY ASSAY FOR HRP ⅡANTIGEN IN PLASMODIUM FALCIPARUM
    胶体金层析法检测恶性疟原虫HRPII抗原的应用研究
短句来源
    Expression and Immunocompetence Characterization of Plasmodium falciparum Lactate Dehydrogenase
    恶性疟原虫乳酸脱氢酶的表达及免疫活性鉴定
短句来源
更多       
  “恶性疟原虫”译为未确定词的双语例句
    STUDY ON DETECTION OF P. FALCIPARUM IN BLOOD SAMPLES WITH BIOTINYLATED PREP20 DNA PROBE
    生物素化质粒rep20探针检测恶性疟原虫感染的研究
短句来源
    The results showed that the sulfoprobe successfully detected 25 pg purified DNA and 0. 001% parasitemia of cultured P. falciparum, and did not react with human leukocyte DNA.
    结果显示:对于体外培养的恶性疟原虫,该法可检出25 pg纯化的DNA,或0.001%的原虫率。
短句来源
    The Plasmodium falcipaium(Pf)DNA fragment was isolatedfrom a cloned recombinant plasmid pPF14,labelled with Digoxigenin(Dig)and used as a probe(pPF14-F-Dig)to detect 274 blood specimens from theendemic area population in Hainan Province by dot hybridization.
    重组的恶性疟原虫DNA片段用洋地黄毒苷配基标记后作为探针(pPF_(14)-F-Dig),以斑点杂交试验对海南省恶性疟流行区人群的274份血样进行检测,并同时作厚薄血片镜检。
短句来源
    Wherever P. falciparum was from Burma or China, it revealed sensitive to fluinine. The ID50 were 608 and 470 nmol/L while ID95 2560 and 1690 nmol/L.
    境内、外恶性疟原虫对奎宁均敏感,ID50分别为608、470nmol/L,ID95为2560,1690nmol/L。
短句来源
    Methods:According to the sequences of the small subunit ribosomal DNA(SSUrDNA) fragment of P.v and P.falciparum ( P.f ) primers were designed and synthetized to establish the multiplex PCR system.
    方法 :以间日疟原虫和恶性疟原虫 (P.f)小亚单位核糖体核糖核酸基因 (SSUr DNA)特定片段为靶基因 ,设计并合成引物 ,建立复合 PCR扩增系统。
短句来源
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  plasmodium falciparum
Design, Synthesis and Evaluation of Trisubstituted Thiazoles Targeting Plasmodium Falciparum Cysteine Proteases
      
The Plasmodium falciparum cysteine proteases, falcipains, have been established as novel targets for antimalarial drug design.
      
The library has been tested against Leishmania donovani and Plasmodium falciparum, with activity at the micromolar level.
      
The identified mutations in the pfcrt, dhfr and dhps genes of Plasmodium falciparum show a very high correlation with resistance to chloroquine, pyrimethamine and sulfadoxine, the drugs that are still used as malaria chemoprophylaxis or treatment.
      
Stellungnahme der ZKBS zur Einstufung der Parasiten Leishmania brasiliensis, Leishmania donovani, Plasmodium falciparum und Tryp
      
更多          
  plasmodium falciparum
Design, Synthesis and Evaluation of Trisubstituted Thiazoles Targeting Plasmodium Falciparum Cysteine Proteases
      
The Plasmodium falciparum cysteine proteases, falcipains, have been established as novel targets for antimalarial drug design.
      
The library has been tested against Leishmania donovani and Plasmodium falciparum, with activity at the micromolar level.
      
The identified mutations in the pfcrt, dhfr and dhps genes of Plasmodium falciparum show a very high correlation with resistance to chloroquine, pyrimethamine and sulfadoxine, the drugs that are still used as malaria chemoprophylaxis or treatment.
      
Stellungnahme der ZKBS zur Einstufung der Parasiten Leishmania brasiliensis, Leishmania donovani, Plasmodium falciparum und Tryp
      
更多          


162 blood samples (dry blood drops) including 51 cases of falciparum malaria and 111 cases of vivax malaria were collected from malaria regions of different endemicities in Yunnan Province. Soluble antigens prepared from a strain of P. Jalciparum isolated from a male patient suffered from subtertian malaria at Simao was cultured to be used in ELISA test. P. cynomolgi antigen was used as control. The result shows that the positive rates of the two antigens are basically identical. However, the GMRT with P. Jalciparum...

162 blood samples (dry blood drops) including 51 cases of falciparum malaria and 111 cases of vivax malaria were collected from malaria regions of different endemicities in Yunnan Province. Soluble antigens prepared from a strain of P. Jalciparum isolated from a male patient suffered from subtertian malaria at Simao was cultured to be used in ELISA test. P. cynomolgi antigen was used as control. The result shows that the positive rates of the two antigens are basically identical. However, the GMRT with P. Jalciparum antigen is significantly higher than that with P. cynomolgi antigen (371.76: 324.68, P<0.05) and the GMRT with homologous antigen is evidently higher than that with heterogeneous antigen (527.8:317.5, P<0.001). When ELISA was applied to 49 blood samples collected from exclusively vivax malaria district, the GMRT of the antibody positivity was markedly higher with P. cynomolgi antigen than with P. falciparum antigen.

本文对采自我省不同疟区162份干血滴,包括恶性疟51例与间日疟111例,采用由当地分离的恶性疟原虫可溶性抗原进行ELISA试验,并用食蟹猴疟原虫(P.cynomolgi)替代抗原作对比,结果显示使用两种抗原的阳性符合率基本一致,但是用P.f抗原的几何平均滴度显著高于用P.c抗原者(371.76,324.68;P<0.05),用P.f抗原测恶性疟患者血清阳性几何平均滴度显著高于用P.c抗原(527.8,383.71:t=1.72 P<0.05)。在单纯间日疟区采集的49份血样使用P.c抗原的抗体阳性几何平均滴度显著高于P.f抗原,前者为388.84,后者为250.79(t=2.91 P<0.01。)

Two monoclonal antibodies(MeAb) 3F_9,M26-32 and pooled sera obtained from mice

本试验采用单克隆抗体(McAb)3F_9、M26-32及约氏疟原虫反复感染的小鼠血清(IMS)为特异性疟疾抗体。抗体经感染红细胞溶解液吸收后,再与固相抗原反应,则抗体结合量减少。抗体结合的抑制程度与受试红细胞中原虫密度相关。以IMS 为反应剂时,本试验可检出约氏疟原虫密度3×10~(-4);恶性疟原虫密度6.4×10~(-4)。用单个McAb 为反应剂时,试验的敏感度较低,但使用两种McAb 混合物可明显改善检测水平。抗原包被方法对试验敏感性也有重大影响。

This paper reports an inhibitory ELISA using monoclonal antibodies M26-32 to detect malaria parasite antigen. The characteristic of this method is to coat the polysterene microculture wells with rabbit anti-mouse IgG serum instead of P. yoelii or P. falci-parum. Examination of 69 microscopically positive and 88 negative blood samples collec-ted on filter paper revealed that, taking inhibition late≥9% as positive standard, the coincidence rate for both microscopically positive and negative samples is above 90%....

This paper reports an inhibitory ELISA using monoclonal antibodies M26-32 to detect malaria parasite antigen. The characteristic of this method is to coat the polysterene microculture wells with rabbit anti-mouse IgG serum instead of P. yoelii or P. falci-parum. Examination of 69 microscopically positive and 88 negative blood samples collec-ted on filter paper revealed that, taking inhibition late≥9% as positive standard, the coincidence rate for both microscopically positive and negative samples is above 90%. The inhibition rate is closely related to parasite density, the lowest detectable level being 2.6 parasites/104 erythrocytes.

以兔抗鼠血清代替约氏疟原虫粗制抗原或恶性疟原虫作包被,采用单克隆抗体M_(26-52)的酶联免疫吸附抑制试验检测恶性疟和间日疟红内期疟原虫抗原,对69份镜检疟原虫阳性和88份镜检阴性滤纸血进行测定,以抑制率≥9%为阳性标准,结果其阳性和阴性样本符合率均超过90%,抑制率与原虫密度呈密切正相关,最低可检出2.6个疟原虫/10~4红细胞。

 
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